Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,840 +GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA intergenic (‑607/‑500) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42338400 (0.000)88 (0.970)
+GCTCGACCAATGGTCTGATTAACCGCTATAAAGCGTGGCGCGGTTA
60/188 0.5 100% intergenic (‑607/‑500) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233841 = NA (NA)intergenic (‑608/‑499) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Only 100 of 156 total aligned reads displayed.

GGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAA  >  NC_000913_3_KO_pgi/4233706‑4233990
                                                                                                                                       |                                                                                                                                                     
cactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATg                                                                                                                                         >  2:268881‑M2/136‑150 (MQ=255)
  ctacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTa                                                                                                                                       <  2:1306065‑M2/17‑1 (MQ=255)
   tacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAg                                                                                                                                      >  4:24956‑M2/133‑150 (MQ=255)
    acggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAgg                                                                                                                                     <  1:718586‑M2/19‑1 (MQ=255)
     cggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGc                                                                                                                                    >  4:717888‑M2/131‑150 (MQ=255)
         gtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGa                                                                                                                                <  3:305811‑M2/24‑1 (MQ=255)
          taggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGAt                                                                                                                               >  3:139489‑M2/126‑150 (MQ=255)
             gctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                            <  3:191662‑M2/28‑1 (MQ=255)
             gctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcagggcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagtgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                            >  1:391392‑M2/123‑150 (MQ=255)
                ggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCg                                                                                                                         >  3:160493‑M2/120‑150 (MQ=255)
                 gagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                                                                        >  2:220017‑M2/119‑150 (MQ=255)
                      gcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcg                                                                                                                   >  2:917778‑M2/114‑150 (MQ=255)
                       gttcgaagttcctattctctagaaagtataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                                  >  3:107861‑M2/113‑150 (MQ=255)
                           gaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                                                                              >  4:689920‑M2/109‑150 (MQ=255)
                           gaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                                                                              >  1:557289‑M2/109‑150 (MQ=255)
                           gaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTCCACGCCGCa                                                                                                              <  2:1020948‑M2/42‑1 (MQ=255)
                             agttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                            <  4:543792‑M2/44‑1 (MQ=255)
                             agttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                                                                            >  1:276056‑M2/107‑150 (MQ=255)
                                   tatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                      >  3:426511‑M2/101‑150 (MQ=255)
                                   tatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                                      >  2:532566‑M2/101‑150 (MQ=255)
                                    atactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                                     <  4:684894‑M2/51‑1 (MQ=255)
                                    atactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAc                                                                                                     <  1:941713‑M2/51‑1 (MQ=255)
                                     tactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAcc                                                                                                    <  3:409068‑M2/52‑1 (MQ=255)
                                      actttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCg                                                                                                   >  1:810041‑M2/98‑150 (MQ=255)
                                       ctttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                                                                                  >  1:721914‑M2/97‑150 (MQ=255)
                                          tctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                                               <  3:702843‑M2/57‑1 (MQ=255)
                                             agagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTg                                                                                            >  3:871116‑M2/91‑150 (MQ=255)
                                                   taggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                                      <  4:872045‑M2/66‑1 (MQ=255)
                                                   taggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                                      >  2:256004‑M2/85‑150 (MQ=255)
                                                    aggaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                                     >  4:544723‑M2/84‑150 (MQ=255)
                                                      gaacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgc                                                                                   >  4:799994‑M2/82‑150 (MQ=255)
                                                       aacttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACCCGATGCCTGATGCGAcgcg                                                                                  <  2:483143‑M2/70‑1 (MQ=255)
                                                         cttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                                >  3:481012‑M2/79‑150 (MQ=255)
                                                         cttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                                                                                <  1:912757‑M2/72‑1 (MQ=255)
                                                          ttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                               >  3:54374‑M2/78‑150 (MQ=255)
                                                          ttcgaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTc                                                                               >  2:502171‑M2/78‑150 (MQ=255)
                                                             gaactgcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgcg                                                                            >  4:750053‑M2/75‑150 (MQ=255)
                                                                  gcaggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                                       <  1:362600‑M2/81‑1 (MQ=255)
                                                                    aggtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgaggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc                                                                     >  3:573025‑M2/68‑150 (MQ=255)
                                                                      gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                                   <  1:282125‑M2/85‑1 (MQ=255)
                                                                      gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                                   >  2:343150‑M2/66‑150 (MQ=255)
                                                                      gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                                   >  1:1362864‑M2/66‑150 (MQ=255)
                                                                      gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                                   >  4:600068‑M2/66‑150 (MQ=255)
                                                                      gtcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGAGACGCGGTCGCGTCTTATCAg                                                                   >  1:374611‑M2/66‑150 (MQ=255)
                                                                       tcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGt                                                                                                                                        <  1:1025588‑M2/16‑1 (MQ=255)
                                                                       tcgacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGt                                                                                                                                        >  2:1025588‑M2/65‑80 (MQ=255)
                                                                         gacggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGgca                                                                <  2:721914‑M2/88‑2 (MQ=255)
                                                                          acggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGCCAAGATAGCGTCTTATCAGGCCt                                                               >  3:460659‑M2/62‑150 (MQ=255)
                                                                          acggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCt                                                               >  2:1119495‑M2/62‑150 (MQ=255)
                                                                          acggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCt                                                               >  2:1136026‑M2/62‑150 (MQ=255)
                                                                            ggatccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc                                                             >  4:591991‑M2/60‑150 (MQ=255)
                                                                               tccccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                          <  4:119709‑M2/94‑1 (MQ=255)
                                                                                  ccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCg                                                       >  2:804875‑M2/54‑150 (MQ=255)
                                                                                  ccggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCg                                                       >  1:1220397‑M2/54‑150 (MQ=255)
                                                                                   cggaatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa                                                      >  4:184886‑M2/53‑150 (MQ=255)
                                                                                      aatgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                                   <  2:557289‑M2/101‑1 (MQ=255)
                                                                                        tgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                                 <  1:268881‑M2/103‑1 (MQ=255)
                                                                                          ctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCTGat                                               <  4:698392‑M2/105‑1 (MQ=255)
                                                                                           tcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                              >  4:81192‑M2/45‑150 (MQ=255)
                                                                                           tcgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                                              >  3:372238‑M2/45‑150 (MQ=255)
                                                                                            cgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCGTCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                                             <  1:170597‑M2/107‑1 (MQ=255)
                                                                                            cgaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGGGTCTTATCAGGCCTACAGGTCGATGCCGatat                                             <  2:1248570‑M2/107‑1 (MQ=255)
                                                                                             gaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg                                            >  1:460186‑M2/43‑150 (MQ=255)
                                                                                             gaccaatggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATg                                            >  4:266846‑M2/43‑150 (MQ=255)
                                                                                                  atggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGGTATGTACAt                                       <  1:975797‑M2/113‑1 (MQ=255)
                                                                                                   tggtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                                      >  1:1248338‑M2/37‑150 (MQ=255)
                                                                                                     gtctgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGt                                    <  4:285917‑M2/116‑1 (MQ=255)
                                                                                                      tctgattaaccgctataaagcgtggcgcggataATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTa                                   >  2:34692‑M2/34‑150 (MQ=255)
                                                                                                        tgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                                 >  2:264744‑M2/32‑150 (MQ=255)
                                                                                                        tgattaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt                                 <  3:717888‑M2/119‑1 (MQ=255)
                                                                                                          attaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg                               <  1:1029627‑M2/121‑1 (MQ=255)
                                                                                                          attaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg                               >  2:1058688‑M2/30‑150 (MQ=255)
                                                                                                          attaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg                               >  3:884581‑M2/30‑150 (MQ=255)
                                                                                                           ttaaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg                              >  3:548077‑M2/29‑150 (MQ=255)
                                                                                                             aaccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGt                                                                           >  2:875397‑M2/27‑103 (MQ=255)
                                                                                                              accgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa                           >  1:1379108‑M2/26‑150 (MQ=255)
                                                                                                               ccgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt                          >  2:1170743‑M2/25‑150 (MQ=255)
                                                                                                                cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                           >  1:1291397‑M2/24‑68 (MQ=255)
                                                                                                                cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                                           <  2:1291397‑M2/45‑1 (MQ=255)
                                                                                                                cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                             >  1:633698‑M2/24‑98 (MQ=255)
                                                                                                                cgctataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                                             <  2:633698‑M2/75‑1 (MQ=255)
                                                                                                                cgctataaagcgtggcgcggttaATCACCGTCGATATCTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                         >  2:784698‑M2/24‑150 (MQ=255)
                                                                                                                cgctataaagcgtggcgcgattaATCATGGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAGGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                         <  2:382058‑M2/127‑1 (MQ=255)
                                                                                                                    ataaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa                     <  3:445262‑M2/131‑1 (MQ=255)
                                                                                                                     taaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                    >  3:597138‑M2/19‑150 (MQ=255)
                                                                                                                     taaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc                    >  3:91579‑M2/19‑150 (MQ=255)
                                                                                                                      aaagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACa                   <  2:566273‑M2/133‑1 (MQ=255)
                                                                                                                       aagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGa                                                                                                                                >  4:366054‑M2/17‑40 (MQ=255)
                                                                                                                       aagcgtggcgcggttaATCATCGTCGATATGTAGGCCGGa                                                                                                                                <  3:366054‑M2/24‑1 (MQ=255)
                                                                                                                        agcgtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATa                 <  3:163430‑M2/135‑1 (MQ=255)
                                                                                                                        agcggggcgcggataATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATACGACAACCGATGCCTGATGCGACGCGGTCGCCTCTTAGCAGGCCTACAGGCCGATGCCGATATGTACATCTGATTCGGCAATTAATACATa                 >  2:980455‑M2/16‑150 (MQ=255)
                                                                                                                           gtggcgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa              >  1:1257664‑M2/13‑150 (MQ=255)
                                                                                                                               cgcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgat          <  1:804195‑M2/142‑1 (MQ=255)
                                                                                                                                gcggttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt         <  1:343150‑M2/143‑1 (MQ=255)
                                                                                                                                   gttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgat      <  3:799994‑M2/146‑1 (MQ=255)
                                                                                                                                   gttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgat      <  3:591991‑M2/146‑1 (MQ=255)
                                                                                                                                    ttaATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgattgatt     <  1:667241‑M2/147‑1 (MQ=255)
                                                                                                                                      aATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTaa   >  2:942536‑M2/2‑150 (MQ=255)
                                                                                                                                       atcatcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTaaa  <  4:208064/150‑1 (MQ=255)
                                                                                                                                       atcatcGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTaaa  >  2:453924/1‑150 (MQ=255)
                                                                                                                                       |                                                                                                                                                     
GGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATTGATTAAA  >  NC_000913_3_KO_pgi/4233706‑4233990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.