BRESEQ :: Evidence

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 781823	147 (1.080)	16 (0.130)	14/258	14.3	10.3%	intergenic (+171/‑262)	lysQ/nadA	tRNA‑Lys/quinolinate synthase, subunit A
*	?	NC_000913_3_KO_pgi	= 781828	144 (1.150)	16 (0.130)	14/258	14.3	10.3%	intergenic (+176/‑257)	lysQ/nadA	tRNA‑Lys/quinolinate synthase, subunit A
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 147 total aligned reads displayed.

TTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTC  >  NC_000913_3_KO_pgi/781679‑781976

 ttttGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                                                                                                                     <  2:564765/150‑1 (MQ=32)

                   tCAAGGATTCGAACCTGCAGCTGGTTTGTGTTGTGCGCCGCGGACCAACGGGGCCGCCCGCGGAGGCGGCCCGAAGAGCGAACGAAGCGCGCCATCCTTCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa                                                                                                                                   <  2:930343/150‑1 (MQ=11)

                   tCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGTGAAGCGAGTCATCCTGCACGACCCCCCAATGTACAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa                                                                                                                                   <  3:664200/150‑1 (MQ=12)

                   tCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa                                                                                                                                   >  2:1200452/1‑150 (MQ=32)

                   tCAAAGAATCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa                                                                                                                                   >  2:1428605/1‑150 (MQ=17)

                    cAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAg                                                                                                                                  <  2:1252396/150‑1 (MQ=32)

                    cAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCAGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAg                                                                                                                                  <  1:1288310/150‑1 (MQ=17)

                     aaaGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAgg                                                                                                                                 >  1:390301/1‑150 (MQ=33)

                     aaaGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCCTTTTTGCTATTCatg                                                                                                                                 >  2:99472/1‑148 (MQ=17)

                         aTTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGGGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCTAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATc                                                                                                                             <  2:22152/150‑1 (MQ=255)

                         aTTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATc                                                                                                                             <  3:320754/150‑1 (MQ=37)

                           tCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCAAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCt                                                                                                                           <  2:1288299/150‑1 (MQ=38)

                            cGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTc                                                                                                                          <  2:1298660/150‑1 (MQ=39)

                             gAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCa                                                                                                                         >  2:717256/1‑150 (MQ=40)

                                   gcagcaGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCa                                                                                                                   <  3:311774/150‑1 (MQ=255)

                                   gcagcaGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGCCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCa                                                                                                                   >  1:1068133/1‑150 (MQ=255)

                                     agcagGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCa                                                                                                                                   >  4:774534/1‑132 (MQ=32)

                                         ggTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGt                                                                                                             <  1:92000/150‑1 (MQ=255)

                                           tttGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTaa                                                                                                           >  3:345954/1‑150 (MQ=255)

                                             tgagttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc                                                                                                         <  1:302609/150‑1 (MQ=255)

                                             tgagttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc                                                                                                         <  1:1248691/150‑1 (MQ=255)

                                             tgagttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc                                                                                                         <  2:695340/150‑1 (MQ=255)

                                             tgagttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAAc                                                                                                         <  1:132733/150‑1 (MQ=255)

                                               agttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCt                                                                                                       <  4:446465/150‑1 (MQ=255)

                                                 ttgagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtga                                                                                                     <  4:345954/150‑1 (MQ=255)

                                                 gtgagGGGAGCGACACAACGGTGAGGGCCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtga                                                                                                     <  2:269360/149‑1 (MQ=17)

                                                   gagCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCCCTTTGTAAACCtgatg                                                                                                   <  3:797331/150‑1 (MQ=255)

                                                         aGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCg                                                                                             >  3:462200/1‑150 (MQ=255)

                                                            gAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGGGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCa                                                                                          <  2:139967/150‑1 (MQ=255)

                                                            gAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCa                                                                                          <  2:287652/150‑1 (MQ=255)

                                                             aaaCAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgat                                                                                                    <  4:375292/139‑1 (MQ=255)

                                                             aaaCAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCag                                                                                         >  1:1257704/1‑150 (MQ=255)

                                                                caacGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGc                                                                                      <  2:461829/150‑1 (MQ=255)

                                                                    ggAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTAc                                                                                  >  2:1196266/1‑150 (MQ=255)

                                                                      aggcGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTGATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACtg                                                                                <  2:559622/147‑1 (MQ=255)

                                                                       gCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACtgt                                                                               >  4:665680/1‑150 (MQ=255)

                                                                        ccGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgatg                                                                                                   <  3:260801/129‑1 (MQ=255)

                                                                        ccGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCtgatg                                                                                                   >  4:260801/1‑129 (MQ=255)

                                                                        ccGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGATTACtgtg                                                                              >  2:1273681/1‑150 (MQ=255)

                                                                         cGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTACAGGCGCTTTTTTTCTAGTCGGGCATCCTCGATGTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgc                                                                             >  3:84631/1‑150 (MQ=21)

                                                                         cGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgc                                                                             >  3:129468/1‑150 (MQ=255)

                                                                          gCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgca                                                                            >  3:363289/1‑150 (MQ=255)

                                                                           cTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaa                                                                           >  1:423396/1‑150 (MQ=255)

                                                                            tCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaag                                                                          >  1:1098763/1‑150 (MQ=255)

                                                                            tCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaag                                                                          >  1:1000776/1‑150 (MQ=255)

                                                                              gcggcgACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaagca                                                                        >  2:827182/1‑150 (MQ=255)

                                                                               cggcgACGGCCCTAAGGGGGAGCGTATCCAGTCCTCCTGCACGACCCACCGATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTgcaagcaa                                                                       <  3:138255/150‑1 (MQ=25)

                                                                                ggcgACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAAc                                                                      >  1:884942/1‑150 (MQ=255)

                                                                                ggcgACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAAc                                                                      >  3:750393/1‑150 (MQ=255)

                                                                                   gACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAActct                                                                   <  4:750393/150‑1 (MQ=255)

                                                                                      ggCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATg                                                                >  2:840373/1‑150 (MQ=255)

                                                                                       gCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGt                                                               >  1:70295/1‑150 (MQ=255)

                                                                                         ccGAAGGGCGAGCGAAGCGAGTCATCCTTCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCg                                                             >  4:101618/1‑150 (MQ=255)

                                                                                           gAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGt                                                           >  2:991915/1‑150 (MQ=255)

                                                                                           gAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGt                                                           <  1:274233/150‑1 (MQ=255)

                                                                                            aaGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCTCCCTAAGGTCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTg                                                          <  1:1072916/150‑1 (MQ=255)

                                                                                            aaGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTg                                                          <  1:999443/150‑1 (MQ=255)

                                                                                            aaGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTg                                                          <  4:534064/150‑1 (MQ=255)

                                                                                            aaGGGCGAGCAAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTg                                                          <  4:489869/150‑1 (MQ=255)

                                                                                               ggCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa                                                       >  4:445778/1‑150 (MQ=255)

                                                                                               ggCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGaa                                                       <  1:829943/150‑1 (MQ=255)

                                                                                                gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAAt                                                      >  3:794060/1‑150 (MQ=255)

                                                                                                gcgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAAt                                                      <  1:1029784/150‑1 (MQ=255)

                                                                                                gcgagcgaAGCGAGTCATCCTGCACGAACCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAAt                                                      >  1:1033514/1‑150 (MQ=255)

                                                                                                 cgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACt                                                                                 <  2:854214/122‑1 (MQ=255)

                                                                                                 cgagcgaAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACt                                                                                 >  1:854214/1‑122 (MQ=255)

                                                                                                      gaagcgaGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCg                                                >  1:1245053/1‑150 (MQ=255)

                                                                                                        agcggGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCAGCCGCATTTTCCTGTTGTAAACCTGGTGACATCGTCAGTGTTTACTGCGCAAGCAACTCTATGTCGGGGGAATTAGGCGTa                                              <  2:649650/150‑1 (MQ=21)

                                                                                                             gTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATg                                         >  3:301781/1‑150 (MQ=255)

                                                                                                              tCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCCTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGa                                        <  1:801417/150‑1 (MQ=255)

                                                                                                                aTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACg                                      >  1:1230101/1‑150 (MQ=255)

                                                                                                                   cTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCAt                                   >  3:728364/1‑150 (MQ=255)

                                                                                                                    tGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATc                                  >  1:302443/1‑150 (MQ=255)

                                                                                                                    tGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATc                                  <  2:423396/150‑1 (MQ=255)

                                                                                                                      cACTACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCt                                <  2:1033359/150‑1 (MQ=255)

                                                                                                                       aCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTg                               <  1:1287689/150‑1 (MQ=255)

                                                                                                                        cGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTGTTGCTATTCAGGCATTCACAATTTCACTTTGTCAACCCGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGc                              >  2:708865/1‑150 (MQ=39)

                                                                                                                         cgcccACCAATGTAAAAAAGCGNCCTANAGGCGNTNTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGca                             <  2:255356/148‑1 (MQ=255)

                                                                                                                            ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGNTATTNANGNATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGNCGCATCCTGcacag                          >  1:255356/1‑149 (MQ=255)

                                                                                                                            ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAgg                                                  <  2:846030/126‑1 (MQ=255)

                                                                                                                            ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACAt                          >  2:565524/1‑150 (MQ=255)

                                                                                                                              accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCACCTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTa                        <  2:884942/150‑1 (MQ=255)

                                                                                                                              accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACATGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTa                        >  1:1111871/1‑150 (MQ=255)

                                                                                                                                   tGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGt                   <  4:293878/150‑1 (MQ=255)

                                                                                                                                      aaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAAt                >  1:257550/1‑150 (MQ=255)

                                                                                                                                      aaaaaaGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAAt                >  2:541574/1‑150 (MQ=255)

                                                                                                                                      aaaaaaGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAAt                >  2:732615/1‑150 (MQ=255)

                                                                                                                                      aaaaaaGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAAt                >  4:512906/1‑150 (MQ=255)

                                                                                                                                      aaaaaaGCGACCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAAt                >  1:666237/1‑150 (MQ=255)

                                                                                                                                       aaaaaGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTAt                                                                 >  4:14612/1‑100 (MQ=255)

                                                                                                                                       aaaaaGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTAt                                                                 <  3:14612/100‑1 (MQ=255)

                                                                                                                                         aaaGCGCCCTAAAGGCGCTTTTTTGCTTTTCATTTATACGCAAGGTCACTTTGTAAACCTGGTGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAGAATGACGCATCCTTCACATTAGGCGTAATTCg             >  2:1145536/1‑150 (MQ=255)

                                                                                                                                           aGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAg           >  2:657716/1‑150 (MQ=255)

                                                                                                                                           aGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAg           <  3:433306/150‑1 (MQ=255)

                                                                                                                                            gcgcCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGt          <  1:1375059/150‑1 (MQ=255)

                                                                                                                                              gcCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGa        <  3:754006/150‑1 (MQ=255)

                                                                                                                                               cccTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGATTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGAc       >  1:874158/1‑150 (MQ=255)

                                                                                                                                                ccTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACt      <  2:390301/150‑1 (MQ=255)

                                                                                                                                                  tAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGCGTGACttt    >  4:584061/1‑150 (MQ=255)

                                                                                                                                                    aaGGCGCTTTTTTTTTTTTTTTTCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTc  >  4:660971/1‑150 (MQ=255)

TTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATTCAGGCATCCTCAATTTCACTTTGTAAACCTGATGACATCGTCAGAGCTTACTGTGCAAGCAACTCTATGTCGGTGGAATTAGGCGTAAAATGACGCATCCTGCACATTAGGCGTAATTCGAGTGACTTTTC  >  NC_000913_3_KO_pgi/781679‑781976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.