Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,757 +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC intergenic (‑524/‑583) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42337570 (0.000)65 (1.100)
+ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC
47/188 0.2 100% intergenic (‑524/‑583) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233758 = 0 (0.000)intergenic (‑525/‑582) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

GCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/4233609‑4233757
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCG  >  NC_000913_3_KO_pgi/4233758‑4233847
                                                                                                                                                     ||||||||||||||||||||||||||||||||||||||||||||||                                                                                          
GCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAA                                                                                                                                         <  4:182509/150‑1
GCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGAGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAA                                                                                                                                         >  4:506240/1‑150
        TAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAGGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAAC                                                                                                                                 <  4:86584/150‑1
          GTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCGTCACAGTTTTCTGTGACTGGCGCTACAATCTTCCACAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACAT                                                                                                                               <  4:210268/150‑1
          GTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACAT                                                                                                                               <  2:562586/150‑1
          GTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACAT                                                                                                                               <  2:514202/150‑1
           TTCCGCTAATAAAGGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCTCTAAAATCATCACATTTTTCTGTGACTGGCGATACAATCTTTCAAAGTCACAATTCTTAAAATCACAAGAGTATTGCAAATGAAAAAAATC                                                                                                                              >  4:124982/1‑150
            TCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCA                                                                                                                             <  2:460488/150‑1
            TCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCA                                                                                                                             <  2:460477/150‑1
              AGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAAT                                                                                                                           >  2:841586/1‑150
                 TAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCA                                                                                                                        >  3:69065/1‑150
                  AATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAA                                                                                                                       <  3:441075/150‑1
                   ATAAATGCTTCACTGCGCTAAGGGCATACACTCACCATTACGCTAACGACAATAAAACCATCACATTTTTCTGTGACTGGCGATACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGGAAAACATCAATCCAAC                                                                                                                      <  2:476274/150‑1
                     AAATGCTTCACTGCGCTAAGGGCTTACACTCAACATTACGCTAACGGCACTAAAAACATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCCCAATTCTCAACATCAGAAGAGTATTGCTAATGAAAAACATAAAGCCAACGC                                                                                                                    >  1:116247/1‑150
                       ATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAG                                                                                                                  >  1:763603/1‑150
                           TTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCCTCACATTTTTCTGTGCCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCG                                                                                                              <  3:292289/150‑1
                           TTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCG                                                                                                              >  2:247449/1‑150
                           TTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCG                                                                                                              >  2:247590/1‑150
                           TTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCG                                                                                                              >  1:800608/1‑150
                            TCACTGCGCTAAGGGTTTACACTCAACATTCCGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGC                                                                                                             <  1:594169/150‑1
                              ACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTG                                                                                                           >  3:247935/1‑150
                               CTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGC                                                                                                          <  4:363405/150‑1
                                  CGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTG                                                                                                       <  1:758748/150‑1
                                  CGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATAGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTG                                                                                                       <  2:285537/150‑1
                                     TAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCA                                                                                                    <  4:103766/150‑1
                                       AGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGG                                                                                                  >  2:821630/1‑150
                                              ACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACG                                                                                           <  1:336433/150‑1
                                                ACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGT                                                                                         <  1:839981/150‑1
                                                ACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGT                                                                                         >  2:608668/1‑150
                                                ACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGT                                                                                         >  2:41885/1‑150
                                                   CAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCAGTACGGTGTA                                                                                      <  1:624756/150‑1
                                                   CAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTA                                                                                      <  4:371711/150‑1
                                                    AACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGTTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAG                                                                                     <  1:539876/150‑1
                                                       ATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCT                                                                                  <  3:243343/150‑1
                                                          ACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGA                                                                               <  3:506240/150‑1
                                                                  GGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCCTC                                                                       >  4:278995/1‑150
                                                                  GGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGT                                                                                         <  3:35731/132‑1
                                                                  GGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGT                                                                                         >  4:35731/1‑132
                                                                   GCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCG                                                                      >  1:366334/1‑150
                                                                      CTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGCTGTAAGCTGGAGCTGCTTCGAAG                                                                   >  2:911101/1‑150
                                                                       TAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGT                                                                  >  1:418055/1‑150
                                                                        AAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTT                                                                 >  4:220686/1‑150
                                                                         AAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTC                                                                <  2:396734/150‑1
                                                                         AAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTC                                                                >  1:742955/1‑150
                                                                           ACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCT                                                              >  3:570871/1‑150
                                                                           ACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCT                                                              <  2:366334/150‑1
                                                                               TCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATAC                                                          >  4:280175/1‑150
                                                                               TCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATAC                                                          >  2:921150/1‑150
                                                                               TCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAA                                                                                                                                    <  3:394989/76‑1
                                                                               TCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAA                                                                                                                                    >  4:394989/1‑76
                                                                                CACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACT                                                         >  2:326417/1‑150
                                                                                  CATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTT                                                       >  1:260388/1‑150
                                                                                   ATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTC                                                      >  4:584689/1‑150
                                                                                    TTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCT                                                     <  3:217280/150‑1
                                                                                    TTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCT                                                     <  4:363696/150‑1
                                                                                          TGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAA                                               >  3:386786/1‑150
                                                                                          TGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAA                                               >  1:719285/1‑150
                                                                                            TGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATA                                             <  2:418055/150‑1
                                                                                            TGACTGGCGCTACAATCTTCCAAAGTCACAATGCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATA                                             <  4:59641/150‑1
                                                                                             GACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAG                                            <  2:805085/150‑1
                                                                                                TGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAA                                         <  1:840362/150‑1
                                                                                                TGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAA                                         >  2:101830/1‑150
                                                                                                     CTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCG                                    <  2:800608/150‑1
                                                                                                      gACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATTCTCTAGAAAGTATAGGAACTTCGA                                   <  4:106617/149‑1
                                                                                                       ACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCCATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAA                                  >  2:451582/1‑150
                                                                                                       ACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAA                                  >  2:320432/1‑150
                                                                                                         AATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACT                                >  3:184180/1‑150
                                                                                                          ATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTG                               <  2:207617/150‑1
                                                                                                          ATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTG                               >  1:295535/1‑150
                                                                                                           TCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGC                              <  2:346381/150‑1
                                                                                                           TCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAG                                                                                     <  4:230265/95‑1
                                                                                                           TCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAG                                                                                     >  3:230265/1‑95
                                                                                                            CTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCA                             <  1:772141/150‑1
                                                                                                             TTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAG                            <  1:483452/150‑1
                                                                                                                 AAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCG                        >  2:318879/1‑150
                                                                                                                 AAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCG                        >  1:805024/1‑150
                                                                                                                   AGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGAC                      >  4:413516/1‑150
                                                                                                                   AGTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGAC                      <  3:126219/150‑1
                                                                                                                    GTCACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACG                     >  2:720960/1‑150
                                                                                                                       ACAATTCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGAT                  >  3:426622/1‑150
                                                                                                                            TCTCAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCG             <  3:413516/150‑1
                                                                                                                               CAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAA          <  3:124982/150‑1
                                                                                                                               CAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGAAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAA          <  1:247449/150‑1
                                                                                                                               CAAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGAAGCCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAA          <  1:247590/150‑1
                                                                                                                                AAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT         >  1:308048/1‑150
                                                                                                                                AAAATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAAT         >  3:72369/1‑150
                                                                                                                                  AATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgc       >  2:788345/1‑148
                                                                                                                                   ATCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgct      <  1:703120/150‑4
                                                                                                                                    TCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctc     >  3:31578/1‑146
                                                                                                                                    TCAGAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctc     <  1:770570/150‑5
                                                                                                                                       GAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgac  <  1:608668/150‑8
                                                                                                                                       GAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgac  <  1:41885/150‑8
                                                                                                                                       GAAGAGTATTGCTAATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCCACGGATCCCCGGAATgctcgac  >  2:726199/1‑143
                                                                                                                                                          AAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTC                                                                <  3:316960/69‑1
                                                                                                                                                          AAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTC                                                                >  4:316960/1‑69
                                                                                                                                                                 AATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACG                     <  2:264874/105‑1
                                                                                                                                                                 AATCCAACGCAGACCGCTGCCTGGCAGGCACTACGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACG                     >  1:264874/1‑105
                                                                                                                                                     ||||||||||||||||||||||||||||||||||||||||||||||                                                                                          
GCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_KO_pgi/4233609‑4233757
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCG  >  NC_000913_3_KO_pgi/4233758‑4233847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.