New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 2904026124 (1.410)8 (0.100) 8/266 11.3 6.5% intergenic (+652/+721) ygcE/queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
?NC_000913_3_KO_pgi = 2904030 113 (1.350)intergenic (+656/+717) ygcE/queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 124 total aligned reads displayed.

TTTTTTGTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACA  >  NC_000913_3_KO_pgi/2903881‑2904179
                                                                                                                                                     |                                                                                                                                                     
   tttGTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGcgcg                                                                                                                                                    <  2:605878/150‑1 (MQ=255)
    ttGTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCgg                                                                                                                                                   <  4:134131/150‑1 (MQ=255)
    ttGTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCgg                                                                                                                                                   <  2:596555/150‑1 (MQ=255)
     tGTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCggg                                                                                                                                                  <  2:586886/150‑1 (MQ=255)
     tGTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCggg                                                                                                                                                  <  4:138202/150‑1 (MQ=255)
      gTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCgggg                                                                                                                                                 <  1:220675/150‑1 (MQ=255)
       tATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGa                                                                                                                                                <  2:487011/150‑1 (MQ=255)
         tCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAAc                                                                                                                                              <  2:729031/150‑1 (MQ=255)
         tCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAAc                                                                                                                                              <  3:209935/150‑1 (MQ=255)
           aaTAATCGTGAACCTCTCTGGCATGAAGAACTATTTTGACAATAAGGTGTACAGTAGATATGTTGGTTATTATTGGTACAACTAATATAATAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTc                                                                                                                                            <  1:548157/149‑1 (MQ=255)
            aTAATTGGGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGGGGATAGGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGGTATATGGTGGTTTATCCCCGCTGGCGCGGGGGACACg                                                                                                                                           >  2:548508/1‑150 (MQ=255)
             tAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTCAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGa                                                                                                                                          <  3:483057/150‑1 (MQ=255)
               aTTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTTGACa                                                                                                                                        <  2:74339/150‑1 (MQ=255)
                ttGTGAACCTCTCTGGCATGGAGAACTATTTTGAACACGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGGTAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACaa                                                                                                                                       >  2:99202/1‑149 (MQ=255)
                   tgAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAAc                                                                                                                                    <  1:114794/150‑1 (MQ=255)
                          ctctGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCa                                                                                                                             <  3:249466/150‑1 (MQ=255)
                            ctGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCagt                                                                                                                           <  3:166872/150‑1 (MQ=255)
                             tGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCagta                                                                                                                          >  1:189751/1‑150 (MQ=255)
                             tGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACAACTAATATGGTGATTTCTCCCCGCTGGCGCGGGGACCTCGACAGAACGGCCTCagta                                                                                                                          >  1:134446/1‑150 (MQ=255)
                              ggCATGGAGAACCATTTTGAACATAAGCTGTTACGTGGATATGTTGCTTATTACAAGTAATGTTAATATAAAAACTTGAGAAAGAGATAACGGGATATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCagtag                                                                                                                         >  4:100966/1‑150 (MQ=255)
                                cATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGtc                                                                                                                       <  3:473617/150‑1 (MQ=255)
                                 aTGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGtct                                                                                                                      >  4:230857/1‑150 (MQ=255)
                                  tGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGtctc                                                                                                                     <  1:614278/150‑1 (MQ=255)
                                      gaACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAACAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGTGGGGAACTCGACAGATCGGCCCCAGTAGTCTCGTCa                                                                                                                 <  2:523482/150‑1 (MQ=255)
                                       aaCTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAg                                                                                                                >  4:379023/1‑150 (MQ=255)
                                           aTTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTc                                                                                                            >  1:765568/1‑150 (MQ=255)
                                           aTTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTc                                                                                                            >  1:363796/1‑150 (MQ=255)
                                            ttttGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAATTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTcc                                                                                                           <  2:132704/150‑1 (MQ=255)
                                               tGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGt                                                                                                        <  2:590733/150‑1 (MQ=255)
                                               tGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGt                                                                                                        <  1:528974/150‑1 (MQ=255)
                                                gAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGtt                                                                                                       >  2:893821/1‑150 (MQ=255)
                                                  aCATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTa                                                                                                     >  1:315146/1‑150 (MQ=255)
                                                  aCATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTa                                                                                                     >  2:734956/1‑150 (MQ=255)
                                                     tGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGACCGGCCTCAGTAGTCTCGTCAGGCTCCGGTCTATcc                                                                                                  >  4:527939/1‑150 (MQ=255)
                                                           gtTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTg                                                                                            <  2:628313/150‑1 (MQ=255)
                                                                gTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGTCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGcgcg                                                                                       >  4:549771/1‑150 (MQ=255)
                                                                gTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGcgcg                                                                                       >  3:1892/1‑150 (MQ=255)
                                                                gTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGcgcg                                                                                       >  3:416085/1‑150 (MQ=255)
                                                                gTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGcgcg                                                                                       >  1:649594/1‑150 (MQ=255)
                                                                 tGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCgg                                                                                      <  2:819107/150‑1 (MQ=255)
                                                                 tGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCgg                                                                                      >  2:580766/1‑150 (MQ=255)
                                                                 tGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACACGACACGCCTGCTTGAGTAGTCTCGTCAGGCGCCGGTTTATCCCCGCTGGCGCgg                                                                                      >  2:580620/1‑150 (MQ=255)
                                                                     tatGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGaa                                                                                  >  2:601284/1‑150 (MQ=255)
                                                                      atGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAac                                                                                 <  1:492302/150‑1 (MQ=255)
                                                                         ttGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGGCCGGTCTCCGTAGTCGCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAacaca                                                                              >  2:452833/1‑149 (MQ=39)
                                                                         ttGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACAcc                                                                              >  3:209519/1‑150 (MQ=255)
                                                                         ttGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACAcc                                                                              >  1:437141/1‑150 (MQ=255)
                                                                            cTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGt                                                                           >  1:635139/1‑150 (MQ=255)
                                                                            cTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGt                                                                           >  1:37121/1‑150 (MQ=255)
                                                                             ttattaCAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGtt                                                                          >  2:149857/1‑150 (MQ=255)
                                                                              tattaCAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGttt                                                                         >  2:469503/1‑150 (MQ=255)
                                                                                ttaCAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGACCGGTGTGAGTAGTTTCGTCAGGCACCGGTTTATCCCCGCTGGCGCGGGGAACACCttacccc                                                                       >  2:695694/1‑144 (MQ=38)
                                                                                     aGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCaaa                                                                  <  1:734956/150‑1 (MQ=255)
                                                                                     aGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCaaa                                                                  <  1:162763/150‑1 (MQ=255)
                                                                                      gTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAAt                                                                 <  4:537973/150‑1 (MQ=255)
                                                                                      gTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAAt                                                                 >  3:36320/1‑150 (MQ=255)
                                                                                       tACTGCTAATATAAAAACTTGAGAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCt                                                               <  4:441793/150‑1 (MQ=255)
                                                                                         cTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTTCCGTTTATCCCCCCTTGCCCGCGGCACACCTGGTTTCGCAAATCTa                                                              >  2:154361/1‑150 (MQ=255)
                                                                                          tGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGGACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTAt                                                             >  4:202930/1‑150 (MQ=255)
                                                                                          tGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTAt                                                             <  4:19492/150‑1 (MQ=255)
                                                                                            cTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATgg                                                           >  3:163915/1‑150 (MQ=255)
                                                                                            cTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATgg                                                           >  1:699391/1‑150 (MQ=255)
                                                                                             tAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGa                                                          >  1:275545/1‑150 (MQ=255)
                                                                                              aaTATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGAc                                                         >  3:425356/1‑150 (MQ=255)
                                                                                              aaTATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGAc                                                         >  3:485705/1‑150 (MQ=255)
                                                                                                tataAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTa                                                       <  2:418837/150‑1 (MQ=255)
                                                                                                  taAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGgcg                                                                                         >  2:680974/1‑114 (MQ=255)
                                                                                                  taAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGgcg                                                                                         <  1:680974/114‑1 (MQ=255)
                                                                                                  taAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGGCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACCAtt                                                     >  3:111135/1‑150 (MQ=255)
                                                                                                   aaaaaCTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAacac                                                                               <  1:523989/123‑1 (MQ=255)
                                                                                                   aaaaaCTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAacac                                                                               >  2:523989/1‑123 (MQ=255)
                                                                                                   aaaaaCTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACACGACAGAACGGCCTCAGTAGTCGCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGCTTTCGCACATCTATGGACTAtag                                                    >  2:802349/1‑148 (MQ=255)
                                                                                                          tGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCg                                             <  4:416085/150‑1 (MQ=255)
                                                                                                              aaaGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGCCTATTGCTATTCGGttt                                         >  3:333741/1‑150 (MQ=255)
                                                                                                              aaaGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGttt                                         <  2:822176/150‑1 (MQ=255)
                                                                                                              aaaGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCGCGTCAGGCTCCGAATTATCCCCGCTCGCGCGGGGAACACCTGGTTTCGCAAATCTATGGACTATTGCTAGTCGGctt                                         >  4:567884/1‑150 (MQ=255)
                                                                                                              aaaGAGATAACGGGTTAGATGGTGGTTTATTCGCGCTGGCTCGGGGAACTAGAAAGAGCGGCGTGAGTAGTCTCGTCAGGCTCAGGTTTATCCCCGCTGGCGCGGGGAACACCTGTATTCGCAAATCTATGGACTATTGGTATACGGttt                                         <  4:520986/150‑1 (MQ=255)
                                                                                                                   gaTAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACAGGACGGAAGAGCGTCGGGAGGGGAGTGAGGGTCCGGGTGAGTGCAGCTCGCGCGGGGCACAGCAGCTTTCGAAAATCTATGGACTATTGCTATTCGGTTTATccc                                    >  4:392420/1‑150 (MQ=255)
                                                                                                                    aTAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTc                                                                                                                                            >  3:179634/1‑45 (MQ=255)
                                                                                                                    aTAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTc                                                                                                                                            <  4:179634/45‑1 (MQ=255)
                                                                                                                    aTAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATcccc                                   >  1:403270/1‑150 (MQ=255)
                                                                                                                      aaCGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTCTTCGGTTTATCCCCGc                                 >  3:389533/1‑150 (MQ=255)
                                                                                                                       aCGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGCACGGCCTCAGTAGTCCCGTCCGGCTCCGGCATAGCCCCGCTGGCGCGGGGAACACCCGTTTTCGCAAATCTATGGCCTATTGCGCTTCGGGTTATCCCCGCt                                >  3:576418/1‑150 (MQ=255)
                                                                                                                        cGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACGGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTg                               >  4:462565/1‑150 (MQ=255)
                                                                                                                         gggTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCGGGCGCGGGGAACACCGTATTTCGCAAATCTATGGACTATTGATATTCGGTTTATCCCCGCggg                              >  4:156686/1‑150 (MQ=255)
                                                                                                                                  ggtggtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGaa                     <  3:2257/150‑1 (MQ=255)
                                                                                                                                  ggtggtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGaa                     >  1:766119/1‑150 (MQ=255)
                                                                                                                                  ggtggtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGGCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCGATTGCCTAGTGCTACGCGGTTTATCCCCGCGGGCggggggca                     >  2:752973/1‑148 (MQ=255)
                                                                                                                                    tggtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCAATTCGGTTTATCCCCGCTGGCGCGGGGAaca                   >  1:919061/1‑150 (MQ=255)
                                                                                                                                     ggtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAacac                  <  3:211778/150‑1 (MQ=255)
                                                                                                                                      gtTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACg                 <  2:275545/150‑1 (MQ=255)
                                                                                                                                        ttATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGCCCATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACggg               >  4:422738/1‑150 (MQ=255)
                                                                                                                                         tATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCGCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGgc              <  2:358716/150‑1 (MQ=255)
                                                                                                                                             cccGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAg                                                                                                                >  1:271535/1‑48 (MQ=255)
                                                                                                                                             cccGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAg                                                                                                                <  2:271535/48‑1 (MQ=255)
                                                                                                                                              ccGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACg         >  1:826540/1‑150 (MQ=255)
                                                                                                                                                gCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGa       >  1:910983/1‑150 (MQ=255)
                                                                                                                                                gCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGa       >  1:189269/1‑150 (MQ=255)
                                                                                                                                                 cTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGaa      <  2:403270/150‑1 (MQ=255)
                                                                                                                                                     cgcgGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACa  <  3:100966/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTTTTGTATCGATAATTGTGAACCTCTCTGGCATGGAGAACTATTTTGAACATGAGGTGTTACGTGGATATGTTGCTTATTACAAGTACTGCTAATATAAAAACTTGAGAAAGAGATAACGGGTTATATGGTGGTTTATCCCCGCTGGCGCGGGGAACTCGACAGAACGGCCTCAGTAGTCTCGTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACA  >  NC_000913_3_KO_pgi/2903881‑2904179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.