New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 232553 =118 (1.340)7 (0.090) 7/256 11.5 6.2% intergenic (+4/+44) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
?NC_000913_3_KO_pgi 232584 = 104 (1.300)intergenic (+35/+13) yafE/mltD putative S‑adenosyl‑L‑methionine‑dependent methyltransferase/putative membrane‑bound lytic murein transglycosylase D
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 119 total aligned reads displayed.

TATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGCTTGAAAGCGAAT  >  NC_000913_3_KO_pgi/232435‑232733
                                                                                                                                                     |                                                                                                                                                     
   tCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTttatta                                                                                                                                                    >  4:276759/1‑150 (MQ=255)
      cATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCt                                                                                                                                                 >  1:813266/1‑150 (MQ=255)
       aTTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTg                                                                                                                                                <  1:763113/150‑1 (MQ=255)
           aCCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGttt                                                                                                                                            >  2:592896/1‑150 (MQ=255)
             cAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGt                                                                                                                                          >  2:299380/1‑150 (MQ=255)
             cAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGt                                                                                                                                          >  1:309003/1‑150 (MQ=255)
                   agCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGaa                                                                                                                                    >  2:770160/1‑150 (MQ=255)
                    gcgcATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGAATAAATACAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGCTTTGTCAGGAAt                                                                                                                                   >  3:200476/1‑150 (MQ=255)
                      gcATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCt                                                                                                                                 <  1:399807/150‑1 (MQ=255)
                       cATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTg                                                                                                                                >  2:770030/1‑150 (MQ=255)
                        aTCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTgg                                                                                                                               >  1:240919/1‑150 (MQ=255)
                        aTCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTgg                                                                                                                               >  1:279965/1‑150 (MQ=255)
                         tCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGc                                                                                                                              >  1:791362/1‑150 (MQ=255)
                          cGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCa                                                                                                                             <  1:129318/150‑1 (MQ=255)
                            aCAGAGGTGAGCACGTATTTTGCCTTGCAGAATGATGGCTTTTTGACCAGTGAGATCATCATGGTAGATGCAGAGAAGGCGGCATAAATAACAAAGGCACCGGGGGAATCGGGGCCTGTATATTAACTTGTTTGTCAGGAATCTGGCAtg                                                                                                                           >  4:1578/1‑150 (MQ=255)
                            aCAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtg                                                                                                                           <  4:417111/150‑1 (MQ=255)
                             cAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgt                                                                                                                          <  1:299380/150‑1 (MQ=255)
                                agGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCCCCAGTGATATCATCATGGTAGATGCACATAAACCGGCATAAATAAAAAAGGCACCCGCGGAATCGGTGCCTTTTTATTATCTGGTTTGTCCGGAATCTGGCAtgttgt                                                                                                                       <  2:229013/150‑1 (MQ=255)
                                agGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttgt                                                                                                                       >  3:384862/1‑150 (MQ=255)
                                     agaACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTg                                                                                                                                                <  2:225923/120‑1 (MQ=255)
                                     agaACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTg                                                                                                                                                >  1:225923/1‑120 (MQ=255)
                                       aaCGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGttttt                                                                                                                >  3:28385/1‑150 (MQ=255)
                                       aaCGTATTTTGCCTTGCAGAATGATGGCTATTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGttttt                                                                                                                <  3:398687/150‑1 (MQ=255)
                                        aCGTTTTTTGCCTTGCAGAATGAGGGCTTTTTCACCAGTTATATCATCATGTTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGTATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTc                                                                                                               <  2:710494/150‑1 (MQ=255)
                                         cGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTca                                                                                                              >  1:601290/1‑150 (MQ=255)
                                         cGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTca                                                                                                              >  1:605314/1‑150 (MQ=255)
                                         cGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTca                                                                                                              <  4:197354/150‑1 (MQ=255)
                                             ttttGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCacaaa                                                                                                          <  1:165343/150‑1 (MQ=255)
                                              tttGCCTTTCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCacaaac                                                                                                         >  2:166587/1‑150 (MQ=255)
                                                   cTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGt                                                                                                    >  1:301337/1‑150 (MQ=255)
                                                        aGAATGATGGCTTTTTCACCAGTGTTTTCATCATGGTAGATGCACATAAAGCGGCATAATTAAAAAAGGCGCTGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGACCGCAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCt                                                                                               >  2:220464/1‑150 (MQ=255)
                                                         gAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttgtt                                                                                                                      >  2:465851/1‑126 (MQ=255)
                                                         caaTGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCAtgttgtt                                                                                                                      <  1:465851/125‑1 (MQ=255)
                                                           atgatgGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTAt                                                                                            <  2:601290/150‑1 (MQ=255)
                                                               tgGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGACATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGcc                                                                                        >  1:223829/1‑150 (MQ=255)
                                                                ggCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCt                                                                                       <  3:169966/150‑1 (MQ=255)
                                                                  cTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTgg                                                                                     <  2:886972/150‑1 (MQ=255)
                                                                        cACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAg                                                                               >  2:864260/1‑150 (MQ=255)
                                                                         aCCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGa                                                                                                                                     >  3:66605/1‑95 (MQ=255)
                                                                         aCCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGa                                                                                                                                     <  4:66605/95‑1 (MQ=255)
                                                                         aCCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGa                                                                              >  1:332397/1‑150 (MQ=255)
                                                                         aCCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGa                                                                              >  2:868061/1‑150 (MQ=255)
                                                                           cAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTGGTTTTTCACAAACAACGTCAGCTTATCGCCGGGTTGCAGAtt                                                                            >  1:408997/1‑150 (MQ=255)
                                                                            agtaatatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTc                                                                           <  1:677375/146‑1 (MQ=255)
                                                                            aGTAATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTc                                                                           <  4:338405/150‑1 (MQ=255)
                                                                              tGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGc                                                                         >  2:702879/1‑150 (MQ=255)
                                                                              tGATATCAGCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCGGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGc                                                                         <  4:200476/150‑1 (MQ=255)
                                                                                atatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATc                                                                                           >  2:246993/1‑130 (MQ=255)
                                                                                atatCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATc                                                                                           <  1:246993/130‑1 (MQ=255)
                                                                                   tcatcaTGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTTAGCTTATCGcc                                                                                        <  2:502428/130‑1 (MQ=255)
                                                                                   tcatcaTGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGcc                                                                                        >  1:502428/1‑130 (MQ=255)
                                                                                        atGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTg                                                               >  2:891936/1‑150 (MQ=255)
                                                                                             aGATGCACATACAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTTCGGAATCAGATATCTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCa                                                          <  3:351207/150‑1 (MQ=255)
                                                                                             aGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCa                                                          <  1:166587/150‑1 (MQ=255)
                                                                                               aTGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAgc                                                        <  2:791362/150‑1 (MQ=255)
                                                                                                 gCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGttt                                                                                                                                            <  2:256082/64‑1 (MQ=255)
                                                                                                 gCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGttt                                                                                                                                            >  1:256082/1‑64 (MQ=255)
                                                                                                 gCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAgcgc                                                      <  2:605314/150‑1 (MQ=255)
                                                                                                  cacaTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGCATCTGGCATGTTGTTGTTTTTCACAAACAACGTCCGCTTATTGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCa                                                     >  1:883889/1‑150 (MQ=255)
                                                                                                  cacaTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCa                                                     <  4:361988/150‑1 (MQ=255)
                                                                                                   acaTAAAGCGGCATAAATAAAAAAGGCACCGGGTGAATCGGGGGCGTTTTATTATCTTTTTGTTCAGGAATCTTGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCAt                                                    <  1:84614/150‑1 (MQ=255)
                                                                                                    caTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATc                                                   >  3:380937/1‑150 (MQ=255)
                                                                                                     aTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATca                                                  >  3:504665/1‑150 (MQ=255)
                                                                                                     aTAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATca                                                  <  2:210539/150‑1 (MQ=255)
                                                                                                       aaaGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATcaca                                                >  1:679659/1‑150 (MQ=255)
                                                                                                         aGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGt                                                                                                    <  3:503518/96‑1 (MQ=255)
                                                                                                         aGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGt                                                                                                    >  4:503518/1‑96 (MQ=255)
                                                                                                          gCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCt                                             >  1:350772/1‑150 (MQ=255)
                                                                                                           cGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCtt                                            <  2:422484/150‑1 (MQ=255)
                                                                                                            ggCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCttt                                           <  2:240919/150‑1 (MQ=255)
                                                                                                             gCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTg                                          >  1:154285/1‑150 (MQ=255)
                                                                                                               ataaataaAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGAt                                        <  3:303335/150‑1 (MQ=255)
                                                                                                               ataaataaAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGAt                                        <  4:154156/150‑1 (MQ=255)
                                                                                                               ataaataaAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGCTATTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGAt                                        <  1:17828/150‑1 (MQ=255)
                                                                                                                 aaataaaAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCt                                                                <  3:472932/124‑1 (MQ=255)
                                                                                                                 aaataaaAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCt                                                                >  4:472932/1‑124 (MQ=255)
                                                                                                                  aataaaAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGtt                                     <  2:301337/150‑1 (MQ=255)
                                                                                                                  aataaaAAAGGCACCGAGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGtt                                     >  3:521308/1‑150 (MQ=255)
                                                                                                                     aaaaaaGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAg                                                                                                 <  1:173734/87‑1 (MQ=255)
                                                                                                                       aaaaGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGc                                >  2:338758/1‑150 (MQ=255)
                                                                                                                             cACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGc                          <  2:795001/150‑1 (MQ=255)
                                                                                                                                cGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCgac                       >  1:402339/1‑148 (MQ=255)
                                                                                                                                 gggggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGttt                      >  1:402878/1‑150 (MQ=255)
                                                                                                                                  gggtAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAAGCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTa                     <  3:12473/150‑1 (MQ=255)
                                                                                                                                  ggggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTa                     <  2:217742/150‑1 (MQ=255)
                                                                                                                                  ggggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTa                     >  4:517755/1‑150 (MQ=255)
                                                                                                                                   gggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAg                    <  3:412977/150‑1 (MQ=255)
                                                                                                                                    ggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGc                   <  2:428648/150‑1 (MQ=255)
                                                                                                                                    ggAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGc                   >  4:51147/1‑150 (MQ=255)
                                                                                                                                     gAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCa                  <  2:756493/150‑1 (MQ=255)
                                                                                                                                        tCGGTGCCTTCAGAATATCTGTTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATg               >  4:119556/1‑150 (MQ=255)
                                                                                                                                         cGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGc              <  1:919340/150‑1 (MQ=255)
                                                                                                                                          ggTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGCt             <  4:384862/150‑1 (MQ=255)
                                                                                                                                          ggTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGCt             <  2:114343/150‑1 (MQ=255)
                                                                                                                                          ggTGCCCTTTTATTATCTAGTTAGACAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGGTTAGCAATGCt             >  4:117024/1‑150 (MQ=255)
                                                                                                                                           gTGCCTTTTAATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGCtt            >  1:624367/1‑150 (MQ=255)
                                                                                                                                             gCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGttttt                                                                                                                <  2:485053/48‑1 (MQ=255)
                                                                                                                                             gCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGa                                         <  4:370658/119‑1 (MQ=255)
                                                                                                                                                tttttATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGCTTGAAAg       <  1:592896/150‑1 (MQ=255)
                                                                                                                                                     attatCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGCTTGAAAGCGAAt  >  2:755647/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATTCGCATTTACCAACAGAGCGCATCGACAGAGGTGAGAACGTATTTTGCCTTGCAGAATGATGGCTTTTTCACCAGTGATATCATCATGGTAGATGCACATAAAGCGGCATAAATAAAAAAGGCACCGGGGGAATCGGTGCCTTTTTATTATCTGGTTTGTCAGGAATCTGGCATGTTGTTGTTTTTCACAAACAACGTCAGCTTATCGCCTGGTTGCAGATTCGCAGTGTCGCTGTTCCAGCGCATCACATCTTTGATGTTCACGCCGTGGCGTTTAGCAATGCTTGAAAGCGAAT  >  NC_000913_3_KO_pgi/232435‑232733

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.