Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_KO_pgi	3,397,657	Δ9 bp	coding (131‑139/1470 nt)	rng ←	ribonuclease G

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_KO_pgi	3397657	3397665	9	75 [0]	[0] 75	rng	ribonuclease G

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 3397656	0 (0.000)	73 (0.880)	58/264	0.5	100%	coding (140/1470 nt)	rng	ribonuclease G
	?	NC_000913_3_KO_pgi	3397666 =	0 (0.000)					coding (130/1470 nt)	rng	ribonuclease G

GATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCAC  >  NC_000913_3_KO_pgi/3397517‑3397813                                                                                                                                                      |                                                                                                                                                    cagttccgagatgtcacgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacc                                                                                                                                                     >  1:289173‑M2/150‑150 (MQ=255)  agttccgagatgtcgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgaccc                                                                                                                                                    >  1:480140‑M2/149‑150 (MQ=255)   gttccgagatgtcgcgcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCt                                                                                                                                                   >  1:725785‑M2/148‑150 (MQ=255)      ccgagatggtgcgcagcgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGt                                                                                                                                                <  2:480140‑M2/6‑1 (MQ=255)                  gcaccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagacacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCcta                                                                                                                                    <  1:28618‑M2/18‑1 (MQ=255)                    accgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCctact                                                                                                                                  >  1:37511‑M2/131‑150 (MQ=255)                     ccgtgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCctacta                                                                                                                                 >  2:445817‑M2/130‑150 (MQ=255)                        tgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctgcaaaagccgcctgcatacccggcagtacacgacCCTTGTAGATATTGCCTACTATTc                                                                                                                              >  2:448753‑M2/127‑150 (MQ=255)                        tgaattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTc                                                                                                                              >  3:465122‑M2/127‑150 (MQ=255)                          aattgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCcg                                                                                                                            >  2:367035‑M2/125‑150 (MQ=255)                           attgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCcgc                                                                                                                           <  1:819266‑M2/27‑1 (MQ=255)                             tgcttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGt                                                                                                                         <  1:691601‑M2/29‑1 (MQ=255)                               cttttgttcttcacccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTcg                                                                                                                       >  2:774660‑M2/120‑150 (MQ=255)                                           acccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTc                                                                                                           >  3:556123‑M2/108‑150 (MQ=255)                                           acccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTc                                                                                                           >  1:515313‑M2/108‑150 (MQ=255)                                            cccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCa                                                                                                          >  3:464500‑M2/107‑150 (MQ=255)                                            cccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCa                                                                                                          >  1:169877‑M2/107‑150 (MQ=255)                                             ccgccccacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagccaaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCaa                                                                                                         <  2:732590‑M2/45‑1 (MQ=255)                                             ccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCaa                                                                                                         >  1:630960‑M2/106‑150 (MQ=255)                                             ccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCaa                                                                                                         >  4:542820‑M2/106‑150 (MQ=255)                                             ccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCaa                                                                                                         <  3:413310‑M2/45‑1 (MQ=255)                                             ccgccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCaa                                                                                                         >  2:235928‑M2/106‑150 (MQ=255)                                               gccacacattcggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAata                                                                                                       >  1:451906‑M2/104‑150 (MQ=255)                                                    acattgggtgtgcggcatggtgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAAt                                                                                                  <  2:462733‑M2/52‑1 (MQ=255)                                                          ggtgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTg                                                                                            >  4:337865‑M2/93‑150 (MQ=255)                                                            tgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCa                                                                                          <  3:389957‑M2/60‑1 (MQ=255)                                                            tgtgcggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCa                                                                                          <  3:278581‑M2/60‑1 (MQ=255)                                                                cggcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCCCACGTTCAATATGAATTTCCTGCAGAAt                                                                                      >  2:895733‑M2/87‑150 (MQ=255)                                                                  gcatgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATAc                                                                                    >  4:86828‑M2/85‑150 (MQ=255)                                                                     tgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGc                                                                                 >  2:696749‑M2/82‑150 (MQ=255)                                                                     tgatgtcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGc                                                                                 <  2:353253‑M2/69‑1 (MQ=255)                                                                          tcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCa                                                                            >  1:58814‑M2/77‑150 (MQ=255)                                                                          tcggatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCa                                                                            >  4:548827‑M2/77‑150 (MQ=255)                                                                             gatgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAata                                                                         <  1:883762‑M2/77‑1 (MQ=255)                                                                               tgcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAAtata                                                                       <  2:160154‑M2/79‑1 (MQ=255)                                                                                gcatgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATAc                                                                      <  3:557093‑M2/80‑1 (MQ=255)                                                                                  atgaagagacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGc                                                                    >  1:321477‑M2/69‑150 (MQ=255)                                                                                  atgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAAATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCCGCATACCGCCATCAATATACGc                                                                    >  4:171888‑M2/69‑150 (MQ=255)                                                                                   tgaagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGcc                                                                   <  1:75785‑M2/83‑1 (MQ=255)                                                                                     aagaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCCCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCAc                                                                 >  2:524654‑M2/66‑150 (MQ=255)                                                                                       gaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACg                                                                                                              <  1:453764‑M2/40‑1 (MQ=255)                                                                                       gaaacgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACg                                                                                                              >  2:453764‑M2/64‑103 (MQ=255)                                                                                          acgcggctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTcgcg                                                            >  4:147102‑M2/61‑150 (MQ=255)                                                                                               gctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAat                                                                                                        <  1:208131‑M2/46‑1 (MQ=255)                                                                                               gctttatccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAat                                                                                                        >  2:208131‑M2/56‑101 (MQ=255)                                                                                                    atccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAgg                                                  <  2:21467‑M2/100‑1 (MQ=255)                                                                                                    atccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAgg                                                  <  1:445817‑M2/100‑1 (MQ=255)                                                                                                      ccagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCg                                                >  2:695275‑M2/49‑150 (MQ=255)                                                                                                       cagcccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGt                                               >  3:418656‑M2/48‑150 (MQ=255)                                                                                                          cccaatatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTAc                                            <  4:144819‑M2/106‑1 (MQ=255)                                                                                                               tatctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTa                                       <  3:337865‑M2/111‑1 (MQ=255)                                                                                                                atctacaaaagccacctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTAc                                      <  2:811274‑M2/112‑1 (MQ=255)                                                                                                                 tctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCACTATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACt                                     >  3:36871‑M2/38‑150 (MQ=255)                                                                                                                 tctacaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACt                                     >  1:21640‑M2/38‑150 (MQ=255)                                                                                                                    acaaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAAc                                  >  2:325371‑M2/35‑150 (MQ=255)                                                                                                                     caaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACa                                 <  1:717060‑M2/117‑1 (MQ=255)                                                                                                                      aaaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACaa                                >  2:208055‑M2/33‑150 (MQ=255)                                                                                                                       aaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGttt                                        >  4:116296‑M2/32‑141 (MQ=255)                                                                                                                       aaagccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGttt                                        <  3:116296‑M2/110‑1 (MQ=255)                                                                                                                         agccgcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTc                             >  4:471555‑M2/30‑150 (MQ=255)                                                                                                                             gcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGcc                         <  3:93899‑M2/125‑1 (MQ=255)                                                                                                                             gcctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGcc                         <  4:465122‑M2/125‑1 (MQ=255)                                                                                                                              cctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGttt                                        <  1:115928‑M2/110‑1 (MQ=255)                                                                                                                              cctgcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGttt                                        >  2:115928‑M2/25‑134 (MQ=255)                                                                                                                                 gcatacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCa                     <  1:288213‑M2/129‑1 (MQ=255)                                                                                                                                  catacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCAt                    <  3:548827‑M2/130‑1 (MQ=255)                                                                                                                                   atacccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATg                   >  1:197396‑M2/20‑150 (MQ=255)                                                                                                                                     acccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGtt                 >  4:57287‑M2/18‑150 (MQ=255)                                                                                                                                     acccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGGTTACTAACAATTCAGCCGTCATGtt                 >  2:191162‑M2/18‑150 (MQ=255)                                                                                                                                     acccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTGCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGtt                 >  2:492196‑M2/18‑150 (MQ=255)                                                                                                                                      cccggaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGttt                >  4:152839‑M2/17‑150 (MQ=255)                                                                                                                                          gaagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATcc            <  2:553520‑M2/138‑1 (MQ=255)                                                                                                                                           aagtacacgacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATccc           <  2:289173‑M2/139‑1 (MQ=255)                                                                                                                                                   gacCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCa   <  1:208055‑M2/147‑1 (MQ=255)                                                                                                                                                    acCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCAc  <  2:169877‑M2/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                    GATGTCGCGCACCGTGAATTGCTTTTGTTCTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACCGCCATCAATATACGCCACTCGCGTTTCCGAAGGCGTTACGTTTACTAACAATTCAGCCGTCATGTTTATCCCTTTTCTCAC  >  NC_000913_3_KO_pgi/3397517‑3397813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑