mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | SvNSRound4_20_60_S2402_L007_R1_001.good.fq | 927,932 | 130,881,733 | 100.0% | 141.0 bases | 150 bases | 96.4% |
| errors | SvNSRound4_20_60_S2402_L007_R2_001.good.fq | 927,932 | 130,881,733 | 100.0% | 141.0 bases | 150 bases | 94.8% |
| errors | SvNSRound4_20_60_S2402_L008_R1_001.good.fq | 592,761 | 84,045,103 | 100.0% | 141.8 bases | 150 bases | 96.3% |
| errors | SvNSRound4_20_60_S2402_L008_R2_001.good.fq | 592,761 | 84,045,103 | 100.0% | 141.8 bases | 150 bases | 94.2% |
| total | 3,041,386 | 429,853,672 | 100.0% | 141.3 bases | 150 bases | 95.5% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913_3_KO_pgi | 4,640,086 | 88.5 | 1.8 | 100.0% | Escherichia coli str. K-12 substr. MG1655, pgi gene KO, complete genome. |
| total | 4,640,086 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 79293 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 298 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.020 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913_3_KO_pgi | 0.75435 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction | 0.1 |
| Junction allow suboptimal matches | FALSE |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.2.6 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 18:28:11 19 Dec 2020 | 18:30:56 19 Dec 2020 | 2 minutes 45 seconds |
| Read alignment to reference genome | 18:30:56 19 Dec 2020 | 18:56:41 19 Dec 2020 | 25 minutes 45 seconds |
| Preprocessing alignments for candidate junction identification | 18:56:41 19 Dec 2020 | 18:58:59 19 Dec 2020 | 2 minutes 18 seconds |
| Preliminary analysis of coverage distribution | 18:58:59 19 Dec 2020 | 19:04:31 19 Dec 2020 | 5 minutes 32 seconds |
| Identifying junction candidates | 19:04:31 19 Dec 2020 | 19:11:06 19 Dec 2020 | 6 minutes 35 seconds |
| Re-alignment to junction candidates | 19:11:06 19 Dec 2020 | 19:17:29 19 Dec 2020 | 6 minutes 23 seconds |
| Resolving best read alignments | 19:17:29 19 Dec 2020 | 19:20:47 19 Dec 2020 | 3 minutes 18 seconds |
| Creating BAM files | 19:20:47 19 Dec 2020 | 19:25:48 19 Dec 2020 | 5 minutes 1 second |
| Tabulating error counts | 19:25:48 19 Dec 2020 | 19:27:47 19 Dec 2020 | 1 minute 59 seconds |
| Re-calibrating base error rates | 19:27:47 19 Dec 2020 | 19:27:52 19 Dec 2020 | 5 seconds |
| Examining read alignment evidence | 19:27:52 19 Dec 2020 | 19:54:43 19 Dec 2020 | 26 minutes 51 seconds |
| Polymorphism statistics | 19:54:43 19 Dec 2020 | 19:54:44 19 Dec 2020 | 1 second |
| Output | 19:54:44 19 Dec 2020 | 19:55:29 19 Dec 2020 | 45 seconds |
| Total | 1 hour 27 minutes 18 seconds | ||
