New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 2662483180 (0.730)18 (0.110)
+CTACAGTGAACAGAACCGTAGGTCGGATAAGGCGTTCACGCCGCATCCG
8/184 23.7 13.3% noncoding (175/266 nt) REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences
?NC_000913_3_KO_pgi = 2662583 NA (NA)coding (741/741 nt) trmJ tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 190 total aligned reads displayed.

GCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATC  >  NC_000913_3_KO_pgi/2662336‑2662631
                                                                                                                                                   |                                                                                                                                                    
ggccTGGGTTTGTGTACTATGTAGGCCTGATAGGACGCATTACGCGGCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCaa                                                                                                                                                    <  4:499866/148‑1 (MQ=11)
 cccTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGGCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAAc                                                                                                                                                   <  2:1086731/150‑1 (MQ=37)
   cTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACgg                                                                                                                                                 <  3:662235/150‑1 (MQ=35)
     ggggTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGGCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCt                                                                                                                                               <  2:599711/150‑1 (MQ=18)
      gggTTCTGTACACTGTAGGCCTGATAAGACGCATTACCCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTg                                                                                                                                              <  4:152953/150‑1 (MQ=17)
        gTTCTGTACATTTTAGGCCTGATAAGAAGCATTACGCGTTGCATCAGTCAACGGCTGTCGGATGCGGCGTGTACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                                                                                                                            <  2:1774553/150‑1 (MQ=11)
        gTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                                                                                                                            <  2:1286541/150‑1 (MQ=33)
         ttCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCg                                                                                                                                           >  4:353059/1‑150 (MQ=34)
            tGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGTATGCGCTGTGAACCCCTTTTCCGACCCTCGGTTGAGTTCACTGTCGGCAAGATAAGACGCATTACGCGTCGCATTAGGCAACGGGTGTTggtc                                                                                                                                        >  2:767402/1‑148 (MQ=11)
             gTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATg                                                                                                                                       >  2:29922/1‑150 (MQ=37)
              tACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGTCGCATTACGCGTCGCATCAGGCGACGGCTGTAGGATgg                                                                                                                                      >  4:232709/1‑149 (MQ=255)
              tACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCCTTTCGCAACGGCTGTCGGATgc                                                                                                                                      >  1:1996991/1‑150 (MQ=255)
                 acTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATgcggc                                                                                                                                   <  1:460221/150‑1 (MQ=38)
                       ggCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAAc                                                                                                                             <  4:140246/150‑1 (MQ=255)
                         ccTGAGAGGACGCATTACGCGGCGCCGCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGc                                                                                                                           <  4:1450415/150‑1 (MQ=14)
                           tGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                                                                                                                            >  1:706285/1‑131 (MQ=11)
                           tGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                                                                                                                            <  2:706285/131‑1 (MQ=11)
                            gATAAGACGCATTACGCGTCGCATCAGGCAACAGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCtt                                                                                                                        <  3:805020/150‑1 (MQ=255)
                                 gACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                   >  4:591887/1‑150 (MQ=255)
                                  aCGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGa                                                                                                                  <  1:2387168/150‑1 (MQ=255)
                                   cGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGTGTGAACGCCTTATCCGAc                                                                                                                 <  1:1865156/150‑1 (MQ=255)
                                   cGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGAc                                                                                                                 <  1:1823175/150‑1 (MQ=255)
                                       ttACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGAATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAc                                                                                                             >  3:1022829/1‑150 (MQ=255)
                                         aCGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACgg                                                                                                           <  1:2172186/150‑1 (MQ=255)
                                          cgcgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACTCCTTATCCGACCTACGGt                                                                                                          >  1:1261985/1‑150 (MQ=255)
                                           gcgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGtt                                                                                                         <  2:2217225/150‑1 (MQ=255)
                                             ggcGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCt                                                                                                       <  1:2284259/148‑1 (MQ=255)
                                             gTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGCACGCCTTATCCGACCTACGGTTCt                                                                                                       >  3:1606128/1‑150 (MQ=255)
                                                  aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCa                                                                                                  <  1:155788/150‑1 (MQ=255)
                                                   tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAAc                                                                                                                             >  3:1170631/1‑122 (MQ=255)
                                                   tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAAc                                                                                                                             <  4:1171388/122‑1 (MQ=255)
                                                       gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGTATGCGTCTTGAACGCTTTATCCGCCCTACGGTTGTGTTCACTGTa                                                                                             >  2:856902/1‑150 (MQ=17)
                                                       gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTa                                                                                             <  2:2371214/150‑1 (MQ=255)
                                                       gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGACCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTa                                                                                             >  3:1266466/1‑150 (MQ=255)
                                                        cAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCgg                                                                                                                                          <  1:2208446/104‑1 (MQ=32)
                                                              cTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGtt                                                                                                    >  1:1449168/1‑136 (MQ=255)
                                                               tGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                                                                                                                            <  4:941533/95‑1 (MQ=31)
                                                               tGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTc                                                                                                                                            >  3:941533/1‑95 (MQ=31)
                                                                 tCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTa                                                                                                                       >  1:491040/1‑114 (MQ=255)
                                                                   ggATGCGGCGTGAAGGCCTTATCCGCCCTACGGTTCTGTGCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGa                                                                                 <  2:489455/150‑1 (MQ=255)
                                                                    gATGCGGCGTGAAAGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGAc                                                                                >  2:191047/1‑150 (MQ=255)
                                                                          gcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTa                                                                          <  1:8721/150‑1 (MQ=255)
                                                                          gcgTGAACGCCTTATCCGACCTACGGTTCTGGTCAGTGGAGGCCGGATAATGCGCATGACTCTTCTCATCAGTCAACGGCGGTCGGATGCGGCGGGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTa                                                                          >  1:1042850/1‑150 (MQ=14)
                                                                               aaCGCCTTATCCGCCCTGCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGt                                                                     <  2:2344166/150‑1 (MQ=17)
                                                                               aaCGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGcc                                                                                                                          >  3:257311/1‑97 (MQ=255)
                                                                                aCGCCTTATCCGTCCTGCTGTGCTGTGCACTTTAGTCCTGAGAATACGCATGACTCGTCGCATCATGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTc                                                                    <  1:1839833/150‑1 (MQ=11)
                                                                                 cGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCt                                                                                        >  2:472083/1‑129 (MQ=255)
                                                                                  gCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCCACGGTTCTGGTCACTGTAGGCCTGATAAGACGCAATACGCGTctc                                                                  >  4:267003/1‑148 (MQ=255)
                                                                                    cTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGcc                                                                                         <  1:1180325/125‑1 (MQ=255)
                                                                                    cTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCAt                                                                >  1:1320999/1‑150 (MQ=255)
                                                                                    cTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCAt                                                                >  1:1131213/1‑150 (MQ=255)
                                                                                    cTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGAGGCGGCGTGCCCGCCTTATCCGACCTACGGCGCTGTTCACTGTAGGCCTGCTAAGACGCATTACGCGTCGCAt                                                                >  2:1770019/1‑150 (MQ=14)
                                                                                     ttATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATc                                                               >  3:1125751/1‑150 (MQ=255)
                                                                                      tATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCa                                                              <  4:1098409/150‑1 (MQ=39)
                                                                                        tCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCCTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAgg                                                            >  3:1496510/1‑150 (MQ=255)
                                                                                           gACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCaa                                                         >  2:1500212/1‑150 (MQ=38)
                                                                                           gACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCaa                                                         >  1:2575207/1‑150 (MQ=38)
                                                                                              cTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGCTGCGGCGTGACCGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACgg                                                      <  2:1206153/150‑1 (MQ=25)
                                                                                              cTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACgg                                                      <  2:2433072/150‑1 (MQ=35)
                                                                                              cTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACgg                                                      <  3:1414188/150‑1 (MQ=35)
                                                                                               tACGGTTCTGTTCACTGCAGGCCGGATAAGACGCATTCCGCGGCGGATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGc                                                     <  2:1376578/150‑1 (MQ=11)
                                                                                                  ggTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGt                                                  >  3:541392/1‑150 (MQ=32)
                                                                                                  ggTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGt                                                  >  3:453610/1‑150 (MQ=32)
                                                                                                      cTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGTGTGAACGTCTTATCCGATCTACGGTTCTGTTCAGCGTAGTGCTCATAATACGCATTACGCGTCGCATCCGGCACCGGCTGTTAtt                                              >  2:2548845/1‑150 (MQ=11)
                                                                                                       tGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTc                                             <  3:591887/150‑1 (MQ=35)
                                                                                                         ttCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTa                                                                                             <  3:199134/100‑1 (MQ=32)
                                                                                                          tCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACCCGTCTCTTCAGGCAACGGCGGTTATTCggg                                          >  2:1315610/1‑149 (MQ=11)
                                                                                                           cACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGcc                                         <  1:2363783/150‑1 (MQ=37)
                                                                                                              tGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTg                                      >  3:332025/1‑150 (MQ=39)
                                                                                                               gTAGGCCTGAGAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGt                                     <  1:365765/150‑1 (MQ=255)
                                                                                                                tAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGtt                                    >  3:54518/1‑150 (MQ=40)
                                                                                                                tAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCAGTCGCGGCGTGTACACCTTTTCCGACCTACGATACTGGCCACTGTACGGCCGATGAGACGCATCTCCCGTCGCTTCGGGCACCCGCTGTTATTCCGCCTTGtt                                    >  2:1156742/1‑150 (MQ=2)
                                                                                                                    ccTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCCACTGCTGTTATTCGGCCTTGTTACCt                                <  1:1934288/150‑1 (MQ=255)
                                                                                                                      tGATAAGCCGCATTGCGCGTCGCATCAGGCAACGGCTGTCGGAGGCGGCGGGAACGCCTTATCCGCCTTTCGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTCCGCGTCGCACCCGGCACCGGCTGTTACTCAGCCTTGTTCCCttt                              >  2:1435519/1‑150 (MQ=11)
                                                                                                                      tGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCttt                              >  1:2092017/1‑150 (MQ=255)
                                                                                                                      tGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATGACGCGTCGCATCAGGCAACGGCTGTTAGTTGGGCTTGTTACGttt                              >  2:897474/1‑150 (MQ=11)
                                                                                                                        aTAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTAt                            >  3:412536/1‑150 (MQ=255)
                                                                                                                        aTAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTAt                            >  1:2493147/1‑150 (MQ=255)
                                                                                                                            gACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctg                        >  1:2319738/1‑150 (MQ=255)
                                                                                                                            gACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctg                        >  2:1071594/1‑150 (MQ=255)
                                                                                                                              cGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctgct                      <  4:55913/150‑1 (MQ=255)
                                                                                                                                 aTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTgctgct                   >  3:76413/1‑150 (MQ=255)
                                                                                                                                 aTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTgctgct                   <  4:810177/150‑1 (MQ=255)
                                                                                                                                 aTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTgctgct                   <  2:499147/150‑1 (MQ=255)
                                                                                                                                 aTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTgctgct                   <  4:1600781/150‑1 (MQ=255)
                                                                                                                                   tACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCa                 >  3:462809/1‑150 (MQ=255)
                                                                                                                                      gcgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATa              <  1:1037884/150‑1 (MQ=255)
                                                                                                                                      gcgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATa              <  4:1315425/150‑1 (MQ=255)
                                                                                                                                            cATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTCTACGGCCTTGTTACCCCTTTTCCGCTCCTCGATTGAAGcc        >  2:1051629/1‑150 (MQ=14)
                                                                                                                                            cATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGcc        <  1:737867/150‑1 (MQ=255)
                                                                                                                                             aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCACCGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa       >  2:220329/1‑150 (MQ=255)
                                                                                                                                             aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa       >  3:1324017/1‑150 (MQ=255)
                                                                                                                                             aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa       >  1:1238955/1‑150 (MQ=255)
                                                                                                                                             aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa       <  3:981017/150‑1 (MQ=255)
                                                                                                                                              tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAg      >  3:363810/1‑150 (MQ=255)
                                                                                                                                               cAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCGACGGCTGGTATTCGGCCTTGTTACCTTTGTTCTGCTGCCCTATCGAAGCCAgg     >  4:729498/1‑149 (MQ=17)
                                                                                                                                                 ggCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAAt   <  1:1891504/150‑1 (MQ=255)
                                                                                                                                                  gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCg                                            >  3:1155321/1‑108 (MQ=35)
                                                                                                                                                  gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCg                                            <  4:1155321/108‑1 (MQ=35)
                                                                                                                                                  gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCt                                >  4:1495580/1‑120 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAATC  >  NC_000913_3_KO_pgi/2662336‑2662631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.