New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 4541124219 (0.890)65 (0.280) 49/264 11.5 23.4% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913_3_KO_pgi = 4541420 220 (0.960)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
Rejected: Coverage evenness skew score above cutoff.

Only 100 of 221 total aligned reads displayed.

TGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTT  >  NC_000913_3_KO_pgi/4540976‑4541273
                                                                                                                                                    |                                                                                                                                                      
  aattATACCGCAAGAAATGCAGCACGATTTGACAGATTATGGGAAAGAAATAATATAAAAAAAAAAAAAAAAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAAc                                                                                                                                                     <  4:773427/148‑1 (MQ=255)
   gTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACt                                                                                                                                                    >  1:308357/1‑150 (MQ=255)
   gTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACt                                                                                                                                                    >  4:1468489/1‑150 (MQ=255)
      ataCCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTc                                                                                                                                                 >  4:244736/1‑150 (MQ=255)
        aCCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGTGGCCAAACTGTCCa                                                                                                                                               >  4:1151684/1‑150 (MQ=255)
          cGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCata                                                                                                                                             >  1:1861016/1‑150 (MQ=255)
            ccAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATc                                                                                                                                           <  3:1319205/150‑1 (MQ=255)
                tAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCa‑taa                                                                                                                                      >  4:975818/1‑150 (MQ=255)
                 aaTGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCa‑t                                                                                                                                        <  3:1110746/147‑1 (MQ=255)
                        ctCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTa                                                                                                                              <  1:2217351/150‑1 (MQ=255)
                         tCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTAc                                                                                                                             <  1:2458926/150‑1 (MQ=255)
                             tttGCCGGATTATTGTAAAGAAATAATCTCGTAGACGAAAAGGTAAAAAATAAAGAGGGGGGAGTTGACGTATGGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGCGCCAAACGGTCCATATCA‑TAAATAAGTTACTTAt                                                                                                                         >  2:248385/1‑150 (MQ=255)
                             tttGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTAt                                                                                                                         >  3:1219679/1‑150 (MQ=255)
                                gCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTAtttt                                                                                                                      >  1:2477170/1‑150 (MQ=255)
                                  cGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTAtttttt                                                                                                                    <  3:397390/150‑1 (MQ=255)
                                       tATGTGAAAGAAATAATCAAAAAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCaa                                                                                                               <  2:206597/150‑1 (MQ=255)
                                         tGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGc                                                                                                             >  1:2177373/1‑150 (MQ=255)
                                          gggAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCatat                                                                                                                                            <  2:2352870/119‑1 (MQ=255)
                                          gggAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCa                                                                                                            >  3:556366/1‑150 (MQ=255)
                                          gggAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCa                                                                                                            >  3:751528/1‑150 (MQ=255)
                                            gaaagaaaTAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATa                                                                                                          >  3:1524409/1‑150 (MQ=255)
                                               agaaaTAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCaa                                                                                                               >  1:55617/1‑142 (MQ=255)
                                                 aaaTAATGTAATAAATGAAAAAGTAAAAAGAGAAGAGGTTTGATTTAACCTATTGATAAAAAACTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAat                                                                                                     <  4:307328/150‑1 (MQ=255)
                                                          cATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTaaaaaa                                                                                            <  1:1522922/150‑1 (MQ=255)
                                                          cATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTAATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGACAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAAAATAAAAATATTaaaaaa                                                                                            <  2:682414/150‑1 (MQ=255)
                                                            tAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACg                                                                                          >  2:536003/1‑150 (MQ=255)
                                                            tAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTAAGTATTTTTTCTCAAGCATAAAAATATTAAAAAACg                                                                                          >  1:1557847/1‑150 (MQ=255)
                                                            tAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAAAAAAGATAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TGAATAAGTTACGTATTTTTTCTCAAGCAAAAAAAAATTAAAAAACg                                                                                          <  4:488369/150‑1 (MQ=255)
                                                             aaaCGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGa                                                                                         <  2:1109084/150‑1 (MQ=255)
                                                               aCGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACa                                                                                       >  3:1202551/1‑150 (MQ=255)
                                                                cGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACaa                                                                                      >  2:1967895/1‑150 (MQ=255)
                                                                 gAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACaaa                                                                                     >  2:962222/1‑150 (MQ=255)
                                                                    aaaTTAAAAAGAAAAAAGGTTTAATTTAACTTATTGATAATAAAGTTAAAAAAAAAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAAAAAAAAAAAg                                                                                  <  2:2370676/150‑1 (MQ=255)
                                                                     aaTTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGc                                                                                 <  1:1212574/150‑1 (MQ=255)
                                                                       ttAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCAt                                                                               <  1:2306450/150‑1 (MQ=255)
                                                                         aaaaaGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCt                                                                             <  3:508753/150‑1 (MQ=255)
                                                                         aaaaaGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCt                                                                             >  4:635623/1‑150 (MQ=255)
                                                                          aaaaGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATGA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAAAGACAAAAAGCATCTa                                                                            >  1:1238726/1‑150 (MQ=255)
                                                                           aaaGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCatat                                                                                                                                            >  3:1425772/1‑86 (MQ=255)
                                                                           aaaGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTaa                                                                           >  1:1748064/1‑150 (MQ=255)
                                                                           aaaGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTAAAGAATAAAAATATTAAAAAACGACAAAAAGCATCTaa                                                                           <  3:1483700/150‑1 (MQ=255)
                                                                                gaagagGTTTTATCTAACATAATGATAAAAAAAGAAAAAAAATAAATAAATATTAGACAAGTGGGGCCAAACTGTCCATATCA‑TAAAGAAGTTAAGTCTTCTTTCTCAAGCATAAATATATTAAAAAAAAAAAAAAAGCATCTAACTGtt                                                                      <  2:1522494/150‑1 (MQ=255)
                                                                                  agagGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTg                                                                    <  1:2371353/150‑1 (MQ=255)
                                                                                      gTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCata                                                                                                                                             <  2:2542472/74‑1 (MQ=255)
                                                                                       tttGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATg                                                               >  2:1242293/1‑150 (MQ=255)
                                                                                       tttGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATg                                                               >  1:646431/1‑150 (MQ=255)
                                                                                       tttGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATg                                                               >  2:408553/1‑150 (MQ=255)
                                                                                       attGATTTAACTTATTGATAATAAAGTTAAAAACACAAATAAATACCAGACAATAGGGGCCAAACTGTCCATATCA‑TAAATAAGTCACGTATATTATCTCCAGCATACATATATTAAAAAACGACAAAAAGCATCTAACTGTTTGACATg                                                               >  2:1755527/2‑150 (MQ=255)
                                                                                            tttAACTTATTGATAATAAAGTTAAAAAAACAAATAAAACAAAGAAAATTGACGCCAACCAGTCC‑TCTCATTCCTTAGGTTATGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAat                                                          <  3:983272/150‑1 (MQ=255)
                                                                                              tAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTa                                                                                                 <  2:623545/109‑1 (MQ=255)
                                                                                              tAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTa                                                                                                 >  1:623545/1‑109 (MQ=255)
                                                                                                 attATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATAAAAAAAAAAAAAAAAAAGCATCTAACTGTTTGATATGTAAATTAttt                                                     <  2:464104/149‑1 (MQ=255)
                                                                                                  ttATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTGACTGTTTGATATGTAAATTAttt                                                     >  1:1329692/1‑149 (MQ=255)
                                                                                                  ttATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTc                                                    >  1:1028621/1‑150 (MQ=255)
                                                                                                   tATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCt                                                   <  4:1123101/150‑1 (MQ=255)
                                                                                                   tATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCt                                                   <  4:1016012/150‑1 (MQ=255)
                                                                                                      tGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTAtt                                                >  1:1793591/1‑150 (MQ=255)
                                                                                                      tGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTAtt                                                <  1:364857/150‑1 (MQ=255)
                                                                                                      tGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATCGGGGCCAAAATGCCCATATCA‑TTATTGAATTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTAtt                                                <  3:735436/150‑1 (MQ=255)
                                                                                                       gATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCa‑ta                                                                                                                                       <  4:1550582/62‑1 (MQ=255)
                                                                                                       gATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCAt                                                                               >  2:1278311/1‑118 (MQ=255)
                                                                                                          aataaAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTaa                                            >  1:1542119/1‑150 (MQ=255)
                                                                                                          aataaAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATATAACTGTTTGATATGTAAATTATTTCTATTGTaa                                            >  1:1298727/1‑150 (MQ=255)
                                                                                                           ataaAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTaaa                                           <  2:2180722/150‑1 (MQ=255)
                                                                                                           ataaAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAAAGAAAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTaaa                                           <  2:693087/150‑1 (MQ=255)
                                                                                                            taaAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAaat                                          >  1:2290166/1‑150 (MQ=255)
                                                                                                            taaAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAaat                                          >  1:1095553/1‑150 (MQ=255)
                                                                                                            taaAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAaat                                          <  2:1542119/150‑1 (MQ=255)
                                                                                                             aaaGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAaatt                                         >  1:1644430/1‑150 (MQ=255)
                                                                                                                 ttAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGAATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAaattaatt                                     <  4:401160/150‑1 (MQ=255)
                                                                                                                  tAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAAttt                                    <  2:65077/150‑1 (MQ=255)
                                                                                                                     aaaaaCAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTcac                                 <  1:894326/150‑1 (MQ=255)
                                                                                                                     aaaaaCAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTcac                                 <  4:1202551/150‑1 (MQ=255)
                                                                                                                      aaaaCAAATAAATACAAGACAATTGGGGCCAATCTGTCCATGTCA‑TAAGAAATTTACGTATTTTTGCTCAAGCATTACAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTcaca                                <  2:553746/150‑1 (MQ=255)
                                                                                                                      aaaaCAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTcaca                                <  1:58615/150‑1 (MQ=255)
                                                                                                                         aCAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCa                             >  4:1136721/1‑150 (MQ=255)
                                                                                                                           aaataaatACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCAcc                           >  1:1690177/1‑150 (MQ=255)
                                                                                                                             ataaataCAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTAc                                                                                                                             >  4:1204274/1‑50 (MQ=255)
                                                                                                                               aaataCAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGc                                                                                 >  4:1251421/1‑92 (MQ=255)
                                                                                                                                aataCAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGat                                                                  <  3:215477/106‑1 (MQ=255)
                                                                                                                                     gaaaCATTTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTatat                 <  4:70680/147‑1 (MQ=255)
                                                                                                                                     aataCAATTGGGGCCAAACTGTCCATATTA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTatat                 <  2:1557847/147‑1 (MQ=255)
                                                                                                                                     aaGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTatat                 <  1:1967895/150‑1 (MQ=255)
                                                                                                                                     aaGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTatat                 <  3:329880/150‑1 (MQ=255)
                                                                                                                                       gACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTca                                  <  1:2091053/131‑1 (MQ=255)
                                                                                                                                       gACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTGTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTca                                  >  2:2091053/1‑131 (MQ=255)
                                                                                                                                           aTTGGGTCAAAACTGTACATATAA‑TAAATTATTTACTTATTTTTTATCAAGTATAAAATTATTATAAAACAAAAAAAAGTATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACCTCACACCCGCTATATGTAAAg           <  2:1357854/150‑1 (MQ=255)
                                                                                                                                           aTTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTATAAAACGACAAAAAGCATCTAACTGTTTGGTATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAg           <  2:1861016/150‑1 (MQ=255)
                                                                                                                                            ttGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGc          <  1:2541793/150‑1 (MQ=255)
                                                                                                                                            ttGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGc          >  2:1011807/1‑150 (MQ=255)
                                                                                                                                             tGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCt         >  1:381103/1‑150 (MQ=255)
                                                                                                                                               gtgCCAAACTGTCCATATCC‑TAAATAAGTTACGTATCTTTTCTCAAGCATAAAAATATTAAAAAACTACAAATAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATCTGTAAAGCTaa       <  4:556366/148‑1 (MQ=255)
                                                                                                                                               gggCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATTTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTaa       <  1:520484/150‑1 (MQ=255)
                                                                                                                                               gggCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTaa       >  2:1631832/1‑150 (MQ=255)
                                                                                                                                               gggCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTaa       <  3:693096/150‑1 (MQ=255)
                                                                                                                                                ggCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAAc      >  3:836812/1‑150 (MQ=255)
                                                                                                                                                ggCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAAc      <  2:1176767/150‑1 (MQ=255)
                                                                                                                                                 gCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAaat                                          >  3:1156574/1‑113 (MQ=255)
                                                                                                                                                  ccAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGt    <  3:1190726/150‑1 (MQ=255)
                                                                                                                                                  ccAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGt    >  2:346273/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGCCAAACTGTCCATATCA‑TAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTT  >  NC_000913_3_KO_pgi/4540976‑4541273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.