Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 1,158,933 G→A W355* (TGG→TGA ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi1,158,9330GA98.7% 812.4 / ‑3.1 228W355* (TGG→TGAptsGfused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (117/106);  total (118/110)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 228 total aligned reads displayed.

GTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGC  >  NC_000913_3_KO_pgi/1158787‑1159079
                                                                                                                                                  |                                                                                                                                                  
 ttGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGt                                                                                                                                                <  2:82341/150‑1 (MQ=255)
   gcgcCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTc                                                                                                                                              <  2:365847/150‑1 (MQ=255)
       cGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGa                                                                                                                                          <  1:1202119/150‑1 (MQ=255)
       cGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGa                                                                                                                                          <  1:132031/150‑1 (MQ=255)
            cTGTACCTCATCCACGCGATTCTGGCACGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGGCTGGTAACAGCAGCAAACTGTGACTGTTCCCGAtcgtc                                                                                                                                     <  1:12608/150‑1 (MQ=255)
             tGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGAtcgtcg                                                                                                                                    <  2:1935797/150‑1 (MQ=255)
             tGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGAtcgtcg                                                                                                                                    <  2:965502/150‑1 (MQ=255)
                acctcatcCACGCGCTTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGACGCGCGAAGGTCAGTCGTTCTCCAACGCTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTa                                                                                                                                 <  1:1303657/147‑1 (MQ=255)
                 catcatCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTAt                                                                                                                                >  3:1527357/1‑150 (MQ=255)
                   tcatcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCg                                                                                                                              >  4:535030/1‑150 (MQ=255)
                    catcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCgg                                                                                                                             >  1:1450555/1‑150 (MQ=255)
                     atcCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGt                                                                                                                            <  4:837768/150‑1 (MQ=255)
                        cACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTAt                                                                                                                         <  1:1109910/150‑1 (MQ=255)
                          cgcgATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGc                                                                                                                       <  2:672053/150‑1 (MQ=255)
                           gcgATTCTGGCAGGCCTGTCATTCCCAATCGGTATGCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAGACTGTGACTGTTCCCGATCGTCGGTATCCCTTGTGCg                                                                                                                      >  4:357434/1‑150 (MQ=255)
                                tCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGt                                                                                                                 <  2:279006/150‑1 (MQ=255)
                                 cTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGtt                                                                                                                <  2:1748814/150‑1 (MQ=255)
                                    gCAGGCCTGGTATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTtac                                                                                                             <  1:982928/150‑1 (MQ=255)
                                    gCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTtac                                                                                                             <  2:409931/150‑1 (MQ=255)
                                          cTGGCATTCCCAATCTGTATTCTTCTGGGTATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACAcc                                                                                                       <  3:483129/150‑1 (MQ=255)
                                               aTTCCCAATCTGTATTCTTCTGGGGATGAGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCtt                                                                                                  >  2:1445993/1‑150 (MQ=255)
                                                 tCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCCTCTTcc                                                                                                <  4:1255067/150‑1 (MQ=255)
                                                  cccAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGt                                                                                                                            <  1:764470/121‑1 (MQ=255)
                                                   ccAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCcgc                                                                                              <  4:767192/150‑1 (MQ=255)
                                                   ccAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGGTATGCGATTGTTTACTACACCATCTTCcgc                                                                                              >  2:1204326/1‑150 (MQ=255)
                                                    cAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGtt                                                                                                                <  1:1051710/131‑1 (MQ=255)
                                                    cAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGtt                                                                                                                >  2:1051710/1‑131 (MQ=255)
                                                    cAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTAGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCcgcg                                                                                             >  4:1303768/1‑150 (MQ=255)
                                                     aaTCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGCTTGTTTACTACACCATCTTCCGCGt                                                                                            >  1:537542/1‑150 (MQ=255)
                                                         tGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTg                                                                                        >  2:915385/1‑150 (MQ=255)
                                                          gTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCgg                                                                                                                                   <  3:999938/106‑1 (MQ=255)
                                                           tATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGAt                                                                                      >  4:1071931/1‑150 (MQ=255)
                                                             ttcttcTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTa                                                                                    >  4:1184668/1‑150 (MQ=255)
                                                              tcttctGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTaa                                                                                   >  2:1994453/1‑150 (MQ=255)
                                                               cttctGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTaaa                                                                                  <  2:2427712/150‑1 (MQ=255)
                                                                ttctGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCt                                                                                         >  4:1221506/1‑142 (MQ=255)
                                                                ttctGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCt                                                                                         <  3:1221506/142‑1 (MQ=255)
                                                                ttctGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGa                                                                                       <  2:552766/144‑1 (MQ=255)
                                                                ttctGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAg                                                                                 >  2:1293769/1‑150 (MQ=255)
                                                                 tctGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGc                                                                                >  1:2143510/1‑150 (MQ=255)
                                                                   tGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGtt                                                                                                                           <  2:729554/105‑1 (MQ=255)
                                                                   tGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCAc                                                                              <  1:1873278/150‑1 (MQ=255)
                                                                    ggggATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACt                                                                             <  3:1275676/150‑1 (MQ=255)
                                                                    ggggATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACt                                                                             >  1:65080/1‑150 (MQ=255)
                                                                     gggATGCGTGACGTTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTg                                                                            >  2:607626/1‑150 (MQ=255)
                                                                       gATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGa                                                                          >  1:924500/1‑150 (MQ=255)
                                                                          gCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCt                                                                       <  2:773284/150‑1 (MQ=255)
                                                                          gCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGGTCGGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCt                                                                       <  1:25065/150‑1 (MQ=255)
                                                                            gTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTtacta                                                                                                           >  4:1477500/1‑112 (MQ=255)
                                                                            gTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTtacta                                                                                                           <  3:1477500/112‑1 (MQ=255)
                                                                            gTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGa                                                                     >  1:1972358/1‑150 (MQ=255)
                                                                            gTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGa                                                                     >  1:1219493/1‑150 (MQ=255)
                                                                               aCGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCTTGCTGATTAAAGCACTGGATCTGaaaa                                                                  <  1:1880277/150‑1 (MQ=255)
                                                                               aCGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGaaaa                                                                  >  4:1030850/1‑150 (MQ=255)
                                                                                    aCGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCg                                                             <  1:202551/150‑1 (MQ=255)
                                                                                    aCGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCg                                                             <  4:1632749/150‑1 (MQ=255)
                                                                                      gtcgtTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGGTCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCggg                                                           <  1:1701647/150‑1 (MQ=255)
                                                                                      gtcgtTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGt                                                                                                                            >  1:1592982/1‑85 (MQ=255)
                                                                                      gtcgtTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGt                                                                                                                            <  2:1592982/85‑1 (MQ=255)
                                                                                        cgtTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTc                                                         <  4:1564070/150‑1 (MQ=255)
                                                                                          ttCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGt                                                       <  3:659222/150‑1 (MQ=255)
                                                                                          ttCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGt                                                       <  3:1118448/150‑1 (MQ=255)
                                                                                          ttCTCGCACGGTCTGATCGACTTCATCGTTATGTCTGGTAACAGCAGCAAACTGTGCCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGt                                                       <  4:502874/150‑1 (MQ=255)
                                                                                            ctcGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTCTCGGTTATGCGCTTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGa                                                     >  2:2405582/1‑150 (MQ=255)
                                                                                             tcGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGaa                                                    <  3:1197162/150‑1 (MQ=255)
                                                                                              cGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAg                                                   <  1:304990/150‑1 (MQ=255)
                                                                                                cACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGAc                                                 >  2:1369198/1‑150 (MQ=255)
                                                                                                 aCGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGCTCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCCCTGGAGCTGAAAACGCCGGGTCGTGAAGAcg                                                >  2:511708/1‑150 (MQ=255)
                                                                                                  cGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGGTCCCTATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGAcgc                                               <  1:1553698/150‑1 (MQ=255)
                                                                                                     tCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCcgc                                                                                              <  2:1879183/100‑1 (MQ=255)
                                                                                                      cTGATCGACTTCATCGTTATGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACt                                           <  2:109274/150‑1 (MQ=255)
                                                                                                        gATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGa                                         <  3:297676/150‑1 (MQ=255)
                                                                                                         aTCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGaa                                        >  2:1735701/1‑150 (MQ=255)
                                                                                                           cGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGa                                      <  3:357434/150‑1 (MQ=255)
                                                                                                           cGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGa                                      <  1:479844/150‑1 (MQ=255)
                                                                                                            gACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGAt                                     >  3:201928/1‑150 (MQ=255)
                                                                                                             aCTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATg                                    >  1:116998/1‑150 (MQ=255)
                                                                                                               gtCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGCTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGACGACCAGACTGGAGATgaa                                  >  2:1408019/2‑148 (MQ=255)
                                                                                                                  aTCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTta                                                                                                              <  4:1523033/71‑1 (MQ=255)
                                                                                                                   tCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGAtt                                                                                                                   <  1:289819/65‑1 (MQ=255)
                                                                                                                   tCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGAtt                                                                                                                   >  2:289819/1‑65 (MQ=255)
                                                                                                                   tCGTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCCTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAg                              <  3:1303768/150‑1 (MQ=255)
                                                                                                                     gTTCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCCACAGCg                            >  2:2479032/1‑150 (MQ=255)
                                                                                                                      ttCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGa                           <  1:729060/150‑1 (MQ=255)
                                                                                                                      ttCTGTCTGGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGa                           >  3:1001529/1‑150 (MQ=255)
                                                                                                                       tctgtctgGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGAc                          <  3:420217/150‑1 (MQ=255)
                                                                                                                        ctgtctgGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACa                         <  1:224863/150‑1 (MQ=255)
                                                                                                                          gtctgGTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGGGCTGATTAAAGCACTGGATCTGAAAATGCCGGGTCGTGAAGACGCGACTGAAGATGCATAAGCGACAgg                       <  4:1363385/150‑1 (MQ=255)
                                                                                                                              ggTAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTAcc                   >  4:414270/1‑150 (MQ=255)
                                                                                                                                tAACAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACa                         >  2:1685716/1‑142 (MQ=255)
                                                                                                                                 aaCAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTg                                                                                           <  3:88067/75‑1 (MQ=255)
                                                                                                                                 aaCAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTg                                                                                           >  4:88067/1‑75 (MQ=255)
                                                                                                                                 aaCAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGc                >  3:1150280/1‑150 (MQ=255)
                                                                                                                                  aCAGCAGCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCg               >  3:901114/1‑150 (MQ=255)
                                                                                                                                   cagcagCAAACTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGa              >  3:580192/1‑150 (MQ=255)
                                                                                                                                           aaCTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCAc      >  3:1636041/1‑150 (MQ=255)
                                                                                                                                             cTGTGACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCg    >  1:2619676/1‑150 (MQ=255)
                                                                                                                                              tgtgACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCgg   >  4:303684/1‑150 (MQ=255)
                                                                                                                                              tgtgACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCgg   >  4:1581601/1‑150 (MQ=255)
                                                                                                                                               gtgACTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTg                                                                                           >  2:2236707/1‑61 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTTGCGCCGATCCTGTACATCATCCACGCGATTCTGGCAGGCCTGGCATTCCCAATCTGTATTCTTCTGGGGATGCGTGACGGTACGTCGTTCTCGCACGGTCTGATCGACTTCATCGTTCTGTCTGGTAACAGCAGCAAACTGTGGCTGTTCCCGATCGTCGGTATCGGTTATGCGATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGC  >  NC_000913_3_KO_pgi/1158787‑1159079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: