New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	3423376 =	96 (1.170)	17 (0.210)	10/274	8.2	16.9%	intergenic (+182/+47)	yhdZ/rrfF	putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
	?	NC_000913_3_KO_pgi	= 4213162	73 (0.900)					intergenic (+3/‑72)	rrfE/yjaA	5S ribosomal RNA of rrnE operon/stress‑induced protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGA  >  NC_000913_3_KO_pgi/4213025‑4213311                                                                                                                                           |                                                                                                                                                      tGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAcc                                                                                                                                           >  2:99194/1‑150 (MQ=38)  gATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAAccc                                                                                                                                          <  1:67524/150‑1 (MQ=38)       aaCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTc                                                                                                                                     <  2:1113514/150‑1 (MQ=255)        aCAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTcc                                                                                                                                    >  1:1399473/1‑150 (MQ=255)          aGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCggcctta                                                                                                                                  >  1:978954/1‑145 (MQ=255)             aTTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAg                                                                                                                               <  2:1117603/150‑1 (MQ=255)                 gCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCg                                                                                                                           <  2:211853/150‑1 (MQ=255)                 gCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCg                                                                                                                           <  1:16766/150‑1 (MQ=255)                   cTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCggg                                                                                                                         <  2:899546/150‑1 (MQ=255)                         gcAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGttttt                                                                                                                   >  1:435061/1‑150 (MQ=255)                            gTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGc                                                                                                                <  1:1197591/150‑1 (MQ=255)                              aGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCat                                                                                                              <  2:880682/150‑1 (MQ=255)                               gcgcGGTGGTCCCACCTGACCCCAACAAGGGC‑AAGCAGTTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGACCTGCCAGGCATCAAATTAGAAAAACCCCGGTCAATAAGGCCGGGGGATTTTGCata                                                                                                             >  1:357137/1‑150 (MQ=17)                                    gtggtCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAat                                                                                                        >  1:962423/1‑150 (MQ=255)                                        tCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAattatt                                                                                                    >  1:25187/1‑150 (MQ=255)                                           cACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCCTACGGCCGGCGTTTTCTGCATATCAATTATTTGc                                                                                                 <  2:135336/150‑1 (MQ=255)                                           cACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGc                                                                                                 >  2:1309746/1‑150 (MQ=255)                                            aCCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAATACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCa                                                                                                <  1:1019321/150‑1 (MQ=255)                                            aCCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCa                                                                                                >  2:728567/1‑150 (MQ=255)                                                   cccATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGaa                                                                                         <  1:794288/150‑1 (MQ=255)                                                    ccATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAg                                                                                        >  2:372402/1‑150 (MQ=255)                                                    ccATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAg                                                                                        <  2:668441/150‑1 (MQ=255)                                                    ccATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAg                                                                                        <  1:736064/150‑1 (MQ=255)                                                    ccATGCCGAACTCACAAG‑TTAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAg                                                                                        <  2:962423/150‑1 (MQ=255)                                                     cATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAgg                                                                                       >  2:481684/1‑150 (MQ=255)                                                        gCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGAGTCGCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCACTTATTTGCATGATGAAGGGaa                                                                                    <  2:1283453/150‑1 (MQ=255)                                                          cGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAAtc                                                                                  >  1:203152/1‑150 (MQ=255)                                                              cTCAGAAG‑TGAAACGCCGTGGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGACTCTCAt                                                                              <  2:357137/150‑1 (MQ=255)                                                              atCAGAAG‑TGAAAACCCCTAGCGCCGATTGTAGTGTGGGGTCTCCCCATGCGAGAGTATGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCAt                                                                              <  2:1418816/149‑1 (MQ=37)                                                                     g‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGtt                                                                       >  2:815193/1‑150 (MQ=255)                                                                     g‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGtt                                                                       >  1:415708/1‑150 (MQ=255)                                                                     g‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGtt                                                                       <  2:415708/150‑1 (MQ=255)                                                                        gAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCt                                                                     >  1:1443695/1‑150 (MQ=255)                                                                         aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCt                                                                     >  2:1348137/1‑149 (MQ=255)                                                                         aaaCGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCt                                                                     <  1:1348137/149‑1 (MQ=255)                                                                                         tgggTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAACAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                    <  1:326293/148‑1 (MQ=255)                                                                                         gtGGGAGTGTGGGGTCTACCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                    <  2:736185/149‑1 (MQ=255)                                                                                         aTGGTAGTGTTGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                    <  1:948644/150‑1 (MQ=255)                                                                                         aTGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATtcgtc                                                    <  2:946008/150‑1 (MQ=255)                                                                                          gtgaaGTGAGGGGTCTCGCCATGCGAGAGTAGGGACGTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAAGCTCATGTCAGTTCTGTATATCCAAATtcgtcg                                                   <  2:517270/146‑1 (MQ=255)                                                                                             tAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTaa                                                >  2:668242/1‑150 (MQ=255)                                                                                             tAGGGTGGGGCCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTaa                                                <  1:295248/150‑1 (MQ=255)                                                                                               gtgtGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATc                                              >  1:407072/1‑150 (MQ=255)                                                                                                tgtgGGGTCTCCCCATGCGAGAGTAGGGAACTGACAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATAAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCa                                             >  1:50997/1‑150 (MQ=255)                                                                                                  tgGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCaaa                                           >  1:1261544/1‑150 (MQ=255)                                                                                                   ggggTTTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAt                                          <  1:372402/150‑1 (MQ=255)                                                                                                    gggTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAAtt                                         >  1:1029904/1‑150 (MQ=255)                                                                                                      gTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGttttt                                                                                                                   >  1:1002947/1‑74 (MQ=255)                                                                                                      gTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGttttt                                                                                                                   <  2:1002947/74‑1 (MQ=255)                                                                                                         tcCCCATGCGAGGGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGt                                    >  2:1115883/1‑150 (MQ=255)                                                                                                         tcCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGt                                    >  1:1338359/1‑150 (MQ=255)                                                                                                         aagaactGCGACGGTAGGGAACTGCCATGCATCAAATTAGACAAACCCCTGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCTTGATGAAGGGAATCTCATGTCAGTTCTGTTTATCTGAATTCGTTGTAAGCAAATTACTGt                                    >  2:1338534/7‑150 (MQ=255)                                                                                                          ccccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTCTATCCAAATTCGTCGTAATCAAATTACTGtt                                   >  2:1241348/1‑150 (MQ=255)                                                                                                           cccATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCCAATTCGTCGTAATCAAATTACTGttg                                  >  1:401040/1‑149 (MQ=255)                                                                                                             cATGCGAGAGTAGGGAACTGTCAGGCATCAAATTAGAAAAACCCCGATCCATAACGCCTGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTcgc                                <  1:42410/150‑1 (MQ=255)                                                                                                             cATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGATTTTTACATATCAATTATTTGCATGATGAACGGAATATCATGTCAGTTCTGTGTATCCAAATTCGTCGTAATAAAATTATTGTTcac                                >  2:399496/1‑148 (MQ=255)                                                                                                              aTGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCAt                                                                              <  2:731325/103‑1 (MQ=255)                                                                                                              aTGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCAt                                                                              >  1:731325/1‑103 (MQ=255)                                                                                                               ggCGAGAGGAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTCGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGa                              <  2:614620/149‑1 (MQ=255)                                                                                                                  gagaGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCt                           <  2:435061/150‑1 (MQ=255)                                                                                                                      gTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaa                       <  1:668242/150‑1 (MQ=255)                                                                                                                       gaGGGCACTGGCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGaaa                      <  1:895739/149‑1 (MQ=255)                                                                                                                          ggAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCa                   >  2:715340/1‑150 (MQ=255)                                                                                                                               tGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCa                                                                               >  2:85731/1‑85 (MQ=255)                                                                                                                               tGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCa                                                                               <  1:85731/85‑1 (MQ=255)                                                                                                                                  cAGTCATCAACTTAGAAAAACCCCGGTCCATAACGCCGGTGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGaa           <  1:386002/150‑1 (MQ=255)                                                                                                                                  cAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATTAACTTACGGGTCGCGATCTTGAAAGCAAACGTGaa           <  1:728567/150‑1 (MQ=255)                                                                                                                                    ggCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGaatt         <  1:1309746/150‑3 (MQ=255)                                                                                                                                      cATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAgtgt       <  1:481684/150‑1 (MQ=255)                                                                                                                                      cATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAgtgt       <  2:1443695/150‑1 (MQ=255)                                                                                                                                       aTCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTTTc      >  1:758198/1‑150 (MQ=255)                                                                                                                                          aaaTTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAgg   >  1:378462/1‑150 (MQ=255)                                                                                                                                           aaTTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTAAGga  >  1:306389/1‑150 (MQ=255)                                                                                                                                           |                                                                                                                                                      TGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAG‑TGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATTAGAAAAACCCCGGTCCATAAGGCCGGGGTTTTTTGCATATCAATTATTTGCATGATGAAGGGAATCTCATGTCAGTTCTGTATATCCAAATTCGTCGTAATCAAATTACTGTTCGCGATCTTGAAAGCAAACGTGAAGTGTCAGGA  >  NC_000913_3_KO_pgi/4213025‑4213311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.