Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 257908 258683 776 72 [0] [0] 69 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 2579070 (0.000)61 (0.790) 50/262 0.7 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_KO_pgi 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

ACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCT  >  NC_000913_3_KO_pgi/257759‑258050
                                                                                                                                                    |                                                                                                                                               
aCGCTTGTGTTAAAAATTTGCTAAAATGGGACAAGTTGGGAAAACAGATGCATCACAACAGGAGATAGCAATAACGTTACCGAGTGGACACCCGATGAGAAAAAAAAAAAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAaggtaa                                                                                                                                                <  2:666237/150‑2 (MQ=255)
 cggttgtgTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAag                                                                                                                                               <  2:63665‑M1/147‑3 (MQ=255)
  gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                              <  1:1087743‑M1/150‑4 (MQ=255)
  gCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACGAGGCCCGTATATTCGTGAAGGTAagt                                                                                                                                              <  1:1087706‑M1/150‑4 (MQ=255)
    ttGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCGCTAGGCCCGTATATTCGTGAAGGTAagtgc                                                                                                                                            >  2:389110‑M1/1‑145 (MQ=255)
      gtgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaa                                                                                                                                          <  2:1021168‑M1/150‑8 (MQ=255)
       tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaa                                                                                                                                         >  2:1287945‑M1/1‑142 (MQ=255)
       tgtTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaa                                                                                                                                         <  1:756798‑M1/150‑9 (MQ=255)
         ttAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGAAGGATGACAGGAGATAGCAATGACGTGACCGAGTGTACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGACGGTAagtgcaaaga                                                                                                                                       >  2:314096‑M1/1‑140 (MQ=255)
          tAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagat                                                                                                                                      <  2:389754‑M1/150‑12 (MQ=255)
                     tAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctt                                                                                                                           >  2:1143870‑M1/1‑128 (MQ=255)
                       aaTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttt                                                                                                                         >  2:364830‑M1/1‑126 (MQ=255)
                       aaTTTCGCCAATTTGGTAAACCAGTTGAATAACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcgaagataatcgattctttt                                                                                                                         >  1:1412024‑M1/1‑126 (MQ=255)
                        aTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                        <  1:279458‑M1/150‑26 (MQ=255)
                        aTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                        <  2:368491‑M1/150‑26 (MQ=255)
                        aTTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGAAATGTAATTACCTATTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                        <  1:413538‑M1/150‑26 (MQ=255)
                        aTTTTGACAATTTGGTAAAACAGTGGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattcttttt                                                                                                                        <  2:768557‑M1/150‑26 (MQ=255)
                           ttGCCAATTTGGTAAAACAGTTGCATGACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATGCGGGAAGTTAagtgcaaagataatcgattctttttcga                                                                                                                     <  2:56819‑M1/150‑29 (MQ=255)
                            tGCCAATCTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAAGTAagttcaaagataatcgattctttttcgat                                                                                                                    <  1:1317360‑M1/150‑30 (MQ=255)
                               cAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgt                                                                                                                 >  1:567025‑M1/1‑118 (MQ=255)
                                 aTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTA                                                                                                                                                 >  2:1308406‑M1/1‑116 (MQ=255)
                                 aTTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTA                                                                                                                                                 <  1:1308406‑M1/116‑1 (MQ=255)
                                  tttGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgaaaaaaaaattgattcttttttgattttctg                                                                                                              >  2:742277‑M1/1‑115 (MQ=255)
                                         aaaCAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcgaagataatcgattctttttcgattgtctggctgtat                                                                                                       <  2:1310334‑M1/150‑43 (MQ=255)
                                                 gCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattc                                                                                                                             >  2:594475‑M1/1‑100 (MQ=255)
                                                 gCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattc                                                                                                                             <  1:594475‑M1/120‑21 (MQ=255)
                                                 gCATCACAACAGGAGATAGCAATGACGTGACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaac                                                                                               <  1:1445732‑M1/150‑51 (MQ=255)
                                                   aTCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCGCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtccacgt                                                                                             >  2:5243‑M1/1‑98 (MQ=255)
                                                                aTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccg                                                                                <  1:444975‑M1/150‑66 (MQ=255)
                                                                     aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGAAAAGCTAattgcaaagataatcgattctttttcgattttctggctgtatgcggcaacgtgaacccggcaccgggagt                                                                           >  1:202641‑M1/1‑80 (MQ=255)
                                                                         aCGTTTCCGATTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgt                                                                       <  1:997770‑M1/150‑75 (MQ=255)
                                                                              gccgAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGAACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaatt                                                                  <  2:1242525‑M1/149‑80 (MQ=255)
                                                                                 gAGTGGATACCCGAAGAGCAGATTGATCAAAAAATTTACCGCAATAGGCCCGTATATTCGTGAAGGTAagtgcaaagataagcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctg                                                               <  1:912030‑M1/150‑83 (MQ=255)
                                                                                  aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                              >  2:1133401‑M1/1‑67 (MQ=255)
                                                                                  aGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctgg                                                              >  1:132787‑M1/1‑67 (MQ=255)
                                                                                       acacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                                                             <  1:1364055‑M1/130‑69 (MQ=255)
                                                                                       acacCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaa                                                                             >  2:1364055‑M1/1‑62 (MQ=255)
                                                                                          cccGAAGAGCAGAGTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtg                                                      <  2:1412024‑M1/150‑92 (MQ=255)
                                                                                            cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtgga                                                    >  1:985902‑M1/1‑57 (MQ=255)
                                                                                            cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattcgggggctggtgga                                                    >  1:993592‑M1/1‑57 (MQ=255)
                                                                                               agcacagatTGATCAAAAAATTTCCCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatgg                                                 <  1:481551‑M1/146‑97 (MQ=255)
                                                                                                  gCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggcgggtggatggagg                                              >  1:578729‑M1/1‑51 (MQ=255)
                                                                                                     gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctgg                                           <  2:132787‑M1/150‑103 (MQ=255)
                                                                                                     gATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggaactgg                                           >  2:1254870‑M1/1‑48 (MQ=255)
                                                                                                       ttGTTCAAAAAATTTCTCGCACTAGGCCCGTATATTCGTGAAGGTAagggcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgacttctggggctggtggatggagctggaa                                         <  2:1429003‑M1/150‑105 (MQ=255)
                                                                                                       ttGAGCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtcgggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaa                                         <  2:202641‑M1/150‑105 (MQ=255)
                                                                                                             aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                   >  2:205645‑M1/1‑40 (MQ=255)
                                                                                                             aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcag                                   >  1:602770‑M1/1‑40 (MQ=255)
                                                                                                              aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                                >  2:1236436‑M1/1‑39 (MQ=255)
                                                                                                              aaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAa                                                                                                                                                <  1:1236436‑M1/40‑2 (MQ=255)
                                                                                                                     tACCGGACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccg                           <  1:687954‑M1/150‑119 (MQ=255)
                                                                                                                        cGCACTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggaaccggaagtgcgtgaattctggggctggtggatggagctggaagctcaggaatcccgttt                        <  2:1224827‑M1/150‑122 (MQ=255)
                                                                                                                           aCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatt                                                                                                                   <  2:1340147‑M1/56‑31 (MQ=255)
                                                                                                                           aCTAGGCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgatt                                                                                                                   >  1:1340147‑M1/1‑26 (MQ=255)
                                                                                                                                gCCCGTATATTCGTGAAGGTAagtgcaaagttgatctattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctaca                >  1:1438260‑M1/1‑21 (MQ=255)
                                                                                                                                gCCCGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgggaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatgccgttttacctaca                >  2:164564‑M1/1‑21 (MQ=255)
                                                                                                                                 cccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacag               <  1:205645‑M1/150‑131 (MQ=255)
                                                                                                                                  ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagt              >  2:367949‑M1/1‑19 (MQ=255)
                                                                                                                                  ccGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagt              >  1:1027306‑M1/1‑19 (MQ=255)
                                                                                                                                   cGTATATTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagtt             <  1:740163‑M1/150‑133 (MQ=255)
                                                                                                                                        atTCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccag        >  1:34143‑M1/1‑13 (MQ=255)
                                                                                                                                         ttCGTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagt       >  1:464359‑M1/1‑12 (MQ=255)
                                                                                                                                            gTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg    <  2:1027306‑M1/150‑142 (MQ=255)
                                                                                                                                            gTGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttg    >  1:1190559‑M1/1‑9 (MQ=255)
                                                                                                                                             tGAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagttggg   >  2:516116‑M1/1‑8 (MQ=255)
                                                                                                                                              gAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt  <  1:367949‑M1/150‑144 (MQ=255)
                                                                                                                                              gAAGGTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt  <  1:314096‑M1/150‑144 (MQ=255)
                                                                                                                                              gAAGGGAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttaccagtttggt  <  2:878594‑M1/150‑144 (MQ=255)
                                                                                                                                                 ggTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacca         >  2:1022943‑M1/1‑4 (MQ=255)
                                                                                                                                                 ggTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgcgtgaattctggggctggtggatggagctggaagcgcaggaatcccgttttacctacagttacca         <  1:1022943‑M1/140‑137 (MQ=255)
                                                                                                                                                  gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgc                                                                         >  2:1165876‑M1/1‑3 (MQ=255)
                                                                                                                                                  gTAagtgcaaagataatcgattctttttcgattgtctggctgtatgcgtcaacgtgaaaccggcaccggaagtgc                                                                         <  1:1165876‑M1/75‑73 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
ACGCTTGTGTTAAAAATTTGCTAAATTTTGCCAATTTGGTAAAACAGTTGCATCACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCT  >  NC_000913_3_KO_pgi/257759‑258050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: