| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913_3_KO_pgi | 4,233,757 | +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC | intergenic (‑524/‑583) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 4233757 | 0 (0.000) | 26 (1.060) +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC |
22/190 | 0.2 | 100% | intergenic (‑524/‑583) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| ? | NC_000913_3_KO_pgi | 4233758 = | 0 (0.000) | intergenic (‑525/‑582) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
GCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCG > NC_000913_3_KO_pgi/4233609‑4233893 | gCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTa > 2:578897/1‑149 (MQ=255) tCAGTAGTTCAGCTAATAAATGCTTCACTGCGATAAGGGTTTACACTCAACATTACGCTAAAGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAGAATCAGAAGAGTATTGCTAatga > 1:538231‑M1/1‑145 (MQ=255) ttCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacat < 1:205629‑M1/149‑12 (MQ=255) cAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatca > 2:487465‑M1/1‑136 (MQ=255) agataatagatgGTTCACTGCTGTGAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaa < 2:162692‑M1/146‑15 (MQ=255) aGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGACACTAAAACCATCACATTTTTCTGTGACTGGCGATACAATATTCCAAAGTCACAATTATCAAAAACAGAAGAGTATAGCCAatgaaaacaatcaa > 2:444752‑M1/1‑135 (MQ=255) gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaa < 1:55311‑M1/148‑15 (MQ=255) gCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaa > 2:55311‑M1/1‑134 (MQ=255) cTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatc < 1:184757‑M1/149‑17 (MQ=255) taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaac > 1:218358‑M1/1‑129 (MQ=255) aTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgca < 2:496208‑M1/149‑24 (MQ=255) gCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTATGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcaga < 1:394277‑M1/149‑26 (MQ=255) ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacccagacc > 2:622215‑M1/1‑122 (MQ=255) tCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccg < 2:357588‑M1/149‑29 (MQ=255) tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgc < 1:487465‑M1/149‑33 (MQ=255) ttACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcact > 1:60760‑M1/1‑105 (MQ=255) acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcgggcactacgg > 2:489105‑M1/1‑101 (MQ=255) acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcagacactacgg > 1:187161‑M1/1‑101 (MQ=255) aaCATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAGATCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgta > 1:183599‑M1/1‑97 (MQ=255) aTTACGCTAACGGCACTAAAACCATCACATTTTTTTTTTTTTTTCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggc > 2:438856‑M1/1‑94 (MQ=255) tAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactccggtgtaggctggagct > 2:346841‑M1/1‑87 (MQ=255) tAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgagg > 2:30809‑M1/1‑78 (MQ=255) aaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggatggagctgcttcgaagttc < 2:538231‑M1/149‑75 (MQ=255) cATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggcgtaggctggagctgcttcgaagttccta > 2:57572‑M1/1‑72 (MQ=255) acaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatact < 2:621329‑M1/149‑82 (MQ=255) caTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactt > 1:452643‑M1/1‑67 (MQ=255) gtgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgcagtgcctaagctttctagagaa > 1:117055‑M1/1‑58 (MQ=255) cTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttc < 1:356882‑M1/149‑102 (MQ=255) tACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatccacccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcg > 1:92516‑M1/1‑47 (MQ=255) cAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaa < 2:218358‑M1/149‑105 (MQ=255) ttCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgca > 2:360156‑M1/1‑40 (MQ=255) aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtc > 2:195695‑M1/1‑36 (MQ=255) aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtc < 1:412414‑M1/149‑114 (MQ=255) aGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcga < 1:439106‑M1/149‑116 (MQ=255) gTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttc < 2:311698‑M1/107‑75 (MQ=255) gTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttc > 1:311698‑M1/1‑33 (MQ=255) tCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctcgcaggcactacggagtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacg > 2:114‑M1/1‑32 (MQ=255) tctcAAAATCAGAAGAGTATTGCAAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatcccc < 1:392474‑M1/149‑125 (MQ=255) ctcAAGATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccg < 1:103674‑M1/149‑126 (MQ=255) ctcAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccg < 2:187161‑M1/149‑126 (MQ=255) gaagaGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcga > 1:529220‑M1/1‑14 (MQ=255) aagaGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgac < 1:195695‑M1/149‑137 (MQ=255) | GCGGTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCG > NC_000913_3_KO_pgi/4233609‑4233893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |