Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_KO_pgi	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_KO_pgi	1299499–1300693	1300697	5–1199	39 [0]	[0] 38	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 1299498	0 (0.000)	38 (1.010)	33/272	0.2	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913_3_KO_pgi	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCT  >  NC_000913_3_KO_pgi/1299355‑1299639                                                                                                                                                |                                                                                                                                              ccaaTTACCGTTAGTTATCATGATCTCCTAGACTTCCTCCTCTCAGCCACATGTTATTTTCGCCACCTCATAATCCCGGGCGCGCCGAAGCACCATTGAAATGATACTATTAATTCCACTGCCTTTGGTAGAGGAACGTGCTaaataat                                                                                                                                          <  2:459112/147‑6 (MQ=255)      tACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTCGAGGTAATTGCTAAataataatca                                                                                                                                     >  2:143186‑M1/1‑139 (MQ=255)         agttagttATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaatt                                                                                                                                  <  2:291333‑M1/149‑14 (MQ=255)              gttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAATGCTAAataataatcaattgttaa                                                                                                                             >  2:330906‑M1/1‑131 (MQ=255)               ttaTAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATACTTCAAATGATATGATTAATTCCACTGTCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaa                                                                                                                            <  2:557900‑M1/149‑20 (MQ=255)                  taAGGATTTCCGTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaatta                                                                                                                         >  1:423962‑M1/1‑127 (MQ=255)                       aTTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtg                                                                                                                    <  1:526861‑M1/149‑28 (MQ=255)                             ttAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttc                                                                                                              >  2:668020‑M1/1‑116 (MQ=255)                                   gcgtttcctctccatCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactact                                                                                                        <  2:223976‑M1/144‑40 (MQ=255)                                                     gTTATTTTCGCCACCTCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaa                                                                                      <  1:583310‑M1/149‑58 (MQ=255)                                                         ttttCGCCACATCATAATCCTGGGCTTGCTGACAACTGCTTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGATAAataataatcaattgttaaattattgtgcatttcactactggcactgtaatcataaaagata                                                                                  >  2:324528‑M1/1‑88 (MQ=255)                                                                     catAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagc                                                                      >  1:111780‑M1/1‑76 (MQ=255)                                                                                gCTTGCTGAAGAATAATTGAAATGATATTATTAATTCAGCTGCCTTTAGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatt                                                           <  2:419053‑M1/149‑85 (MQ=255)                                                                                  tcgcagaagaataattGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttg                                                         >  2:86133‑M1/6‑63 (MQ=255)                                                                                       gaagaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaaatgtcatcagtaatggtagagattgttacccaatctctatttgattaa                                                    >  2:145431‑M1/1‑58 (MQ=255)                                                                                         agaaTAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaat                                                  >  2:123186‑M1/1‑56 (MQ=255)                                                                                                ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccactctctatttgattgaattgaaaga                                           >  2:66999‑M1/1‑49 (MQ=255)                                                                                                ttGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataataaatcgttaaattattgtgcatttcgctactggaactgtcggtaaacaagatagacatgcttagccaagctctaattgattgaattgaaaaa                                           >  1:438616‑M1/1‑49 (MQ=255)                                                                                                     aTGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttt                                      <  1:479936‑M1/149‑106 (MQ=255)                                                                                                         tattatTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctttatttgattgaattgaaagatgtttgtta                                  <  2:390695‑M1/149‑110 (MQ=255)                                                                                                          attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgcttgaattgaaa                                             <  1:424440‑M1/137‑99 (MQ=255)                                                                                                          attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                             <  1:421428‑M1/137‑99 (MQ=255)                                                                                                          attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                             >  2:424440‑M1/1‑39 (MQ=255)                                                                                                          attattAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaa                                             >  2:421428‑M1/1‑39 (MQ=255)                                                                                                            tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                               <  1:86133‑M1/149‑113 (MQ=255)                                                                                                            tattaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaagg                               >  1:315576‑M1/1‑37 (MQ=255)                                                                                                              ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt                                                          <  1:653080‑M1/120‑86 (MQ=255)                                                                                                              ttaATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattt                                                          >  2:653080‑M1/1‑35 (MQ=255)                                                                                                                 aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                          <  1:337550‑M1/149‑118 (MQ=255)                                                                                                                 aTTCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatgg                          >  1:34450‑M1/1‑32 (MQ=255)                                                                                                                   tCCACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggat                        <  2:423962‑M1/149‑120 (MQ=255)                                                                                                                     cACTGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctattagattgaattgaaagaagtttgttaaggcatggatgc                      >  2:7880‑M1/1‑28 (MQ=255)                                                                                                                        ttgttttgataAAGGAAAGGGCAAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagtcaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                   <  2:333604‑M1/145‑125 (MQ=255)                                                                                                                        tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaag                   >  1:118315‑M1/1‑25 (MQ=255)                                                                                                                        tGCCTTTGGTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaataagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgttggttaaggcatggatgcaag                   >  1:156787‑M1/1‑25 (MQ=255)                                                                                                                               ggTAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctataga            <  1:668020‑M1/149‑132 (MQ=255)                                                                                                                                 tAGAGGAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagatt          >  1:146757‑M1/1‑16 (MQ=255)                                                                                                                                      gAAAGTGCTAAataataatcaattgttaaattattgtgcatttcactactggaactgtaatcagaaaagatagacatgcttagccaatctctaattgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgat     >  2:433066‑M1/1‑11 (MQ=255)                                                                                                                                         aGTGCTAAagaataatcaattgttaaattaatgtgcatttcacttctggaactgtaatcagaaaagatagacatgcttagccaatctctatttgattgaattgaaagatgtttgttaaggcatggatgcaagctatagattctgatacg  <  2:383885‑M1/149‑142 (MQ=255)                                                                                                                                                |                                                                                                                                              CAAATTACAGTTAGTTATAAGGATTTCCTTAACTGCTTCTCCTCACCATCATGTTATTTTCGCCACATCATAATCCTGGGCTTGCTGAAGAATAATTGAAATGATATTATTAATTCCACTGCCTTTGGTAGAGGAAAGTGCTAAGGAAGGTGCGAACAAGTCCCTGATATGAGATCATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCT  >  NC_000913_3_KO_pgi/1299355‑1299639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑