Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 257908 258683 776 37 [0] [0] 37 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 2579070 (0.000)36 (0.980) 35/264 0.1 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913_3_KO_pgi 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator;regulator; Surface structures; transcriptional regulator of cryptic csgA gene for curli surface fibers

TGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGT  >  NC_000913_3_KO_pgi/258538‑258830
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cttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                                                                  >  2:574668‑M2/147‑149 (MQ=255)
 gtgtgttaaaaagttgctaaatcttgccaatttggtaaaacagtggcatcacaacaggagatagcaatgatgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTg                                                                                                                                                 <  2:264150‑M2/4‑1 (MQ=255)
              ttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGa                                                                                                                                    <  2:615682‑M2/17‑1 (MQ=255)
                 ctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTc                                                                                                                                 <  1:657465‑M2/20‑1 (MQ=255)
                         tgccaattgggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgctctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                                                                         <  1:455194‑M2/28‑1 (MQ=255)
                             aatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                     >  2:603595‑M2/118‑149 (MQ=255)
                             aatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgaatattcgtgaaggtaAGTGAAAAGATAATAGATTCTTTTTCGATTGt                                                                                                                     >  2:338256‑M2/118‑149 (MQ=255)
                              atttggtaaaacaggtgcatcacaacaggagatagcaaggacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTc                                                                                                                    <  1:103632‑M2/33‑1 (MQ=255)
                                     aaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGt                                                                                                             <  2:181588‑M2/40‑1 (MQ=255)
                                      aaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATCCTTTTTCGATTGTCTGGCTGTa                                                                                                            >  2:470981‑M2/109‑149 (MQ=255)
                                      aaacagtggcaccaccacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTa                                                                                                            <  2:601028‑M2/41‑1 (MQ=255)
                                       aacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                            >  2:71514‑M2/108‑132 (MQ=255)
                                       aacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttttt                                                                                                                            <  1:71514‑M2/25‑1 (MQ=255)
                                       aacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTCTTCGATTGTCTGGCTGTAt                                                                                                           <  1:574668‑M2/42‑1 (MQ=255)
                                              gcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAt                                                                                                                                       <  1:102766‑M2/14‑1 (MQ=255)
                                              gcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAAt                                                                                                                                       >  2:102766‑M2/101‑114 (MQ=255)
                                                       caggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAGGATAATCGATTCTTTGTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                           >  1:229014‑M2/92‑149 (MQ=255)
                                                         ggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCgg                                                                                         <  1:151407‑M2/60‑1 (MQ=255)
                                                            gatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCAc                                                                                      <  1:534286‑M2/63‑1 (MQ=255)
                                                                  aatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCTATTCTCTGGCCGTATCATTCAACGTGAAAAAGGCACCggaga                                                                                >  2:267124‑M2/81‑147 (MQ=255)
                                                                        gttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTATCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTg                                                                          >  1:572451‑M2/75‑149 (MQ=255)
                                                                         ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                       >  1:66944‑M2/74‑119 (MQ=255)
                                                                         ttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGt                                                                                                       <  2:66944‑M2/46‑1 (MQ=255)
                                                                               aggggacaccggaagagcagattgatcaaaaaagttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGGATGCGTCAACGTGAAACCGGCACCGGAAGTGCGGGAATTCTg                                                                   <  2:327370‑M2/82‑1 (MQ=255)
                                                                                gtggaaacccgaagggaggagtgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAAGGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                                  <  2:325766‑M2/83‑1 (MQ=255)
                                                                                      acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtgg                                                            >  1:374928‑M2/61‑149 (MQ=255)
                                                                                         cgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCtggtgg                                                         >  1:534760‑M2/58‑149 (MQ=255)
                                                                                                        tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTATTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTATGGGGCTGGTGGATGGAGCCGGAAgcg                                          >  2:336169‑M2/43‑149 (MQ=255)
                                                                                                              aatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAc                                                                                            <  1:545765‑M2/57‑1 (MQ=255)
                                                                                                              aatttaccgcactaggcccgtatattcgtgaaggcaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAc                                                                                            >  2:545765‑M2/37‑93 (MQ=255)
                                                                                                                       cactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTTATTTTTTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGtttt                           >  2:412155‑M2/28‑149 (MQ=255)
                                                                                                                         ctaggcccgtatattcgtgaaggtaAGTGGAAAGATAATCGATTCTTTTTCGATTGTCGGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                         <  2:143555‑M2/124‑1 (MQ=255)
                                                                                                                             gcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTTTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTAc                     >  2:680214‑M2/22‑149 (MQ=255)
                                                                                                                               ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                           >  2:572878‑M2/20‑93 (MQ=255)
                                                                                                                               ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGt                                                                           <  1:572878‑M2/74‑1 (MQ=255)
                                                                                                                                         gtgaaggtaAGTGCAAAGATAATCGATTATTTCAGAGTTTGCGGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGttt         >  1:680726‑M2/10‑149 (MQ=255)
                                                                                                                                                taAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGt  <  2:680726‑M2/147‑1 (MQ=255)
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TGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGT  >  NC_000913_3_KO_pgi/258538‑258830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: