Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 668,742 C→G E99Q (GAA→CAA)  rsfS ← ribosomal silencing factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi668,7420CG97.5% 125.3 / NA 40E99Q (GAA→CAA) rsfSribosomal silencing factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (17/22);  minor base T (0/1);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAA  >  NC_000913_3_KO_pgi/668600‑668885
                                                                                                                                              |                                                                                                                                               
ggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAg                                                                                                                                           >  1:252807/1‑149 (MQ=255)
ggAATTTCAATCAGCTCGAAGGGCATATCTTTCCGAACACGACGCAGGTACTCGGTAAAGCCGATTTGTACCCAGTCTGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTACAGAGTTTTTCCAGTTGATACac                                                                                                                                           <  2:634604/149‑2 (MQ=255)
        aaTCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATGCAGGCGACGGc                                                                                                                                   >  1:434346/1‑149 (MQ=255)
              cTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCtt                                                                                                                             <  1:673662/149‑1 (MQ=255)
               tCGAAGGGCATCTCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTc                                                                                                                            >  1:423917/1‑149 (MQ=255)
               tCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTc                                                                                                                            <  2:326872/149‑1 (MQ=255)
                             tttCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGCCAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCAATTTGATACACGCGTCGGCCCTCTTCCTGCATgcccggga                                                                                                              >  2:199986/1‑142 (MQ=255)
                                  gAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATc                                                                                                         <  2:187837/149‑1 (MQ=255)
                                    aaaCGACGCATGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATcac                                                                                                       <  2:493914/149‑1 (MQ=255)
                                       cgacgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCCCATc                                                                                                    >  2:92295/1‑149 (MQ=255)
                                                     ggTAAAACCGGTTTGTACCCAGTCCGGTAGTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATGAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                                                                      <  2:354877/149‑1 (MQ=255)
                                                     ggTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGc                                                                                                  <  1:22800/137‑1 (MQ=255)
                                                     ggTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGc                                                                                                  >  2:22800/1‑137 (MQ=255)
                                                     ggTAAAACCGGTTTGTACCCAATCCGGCATTTTCGTTCCCACGGCGACAGGTTGCAGCTTAACGCATTAACTCCAGAGATTTTCCAGTTGATACCGGGGACGGACCTTCTCCTCAATAACGCGGACCATCCAATCGCCCAAATCCCCGa                                                                                      >  2:302258/1‑149 (MQ=255)
                                                                tttGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCg                                                                           >  2:26641/1‑149 (MQ=255)
                                                                  tgttactaGTCCGGCATTTTCGTGCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTTATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGgc                                                                         <  2:304847/142‑1 (MQ=255)
                                                                       ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAACTCCACGCCAATCCAGCCGCCGCCGt                                                                    >  2:187241/1‑149 (MQ=255)
                                                                         aGTCCGGCATTTTCGTTCCCACGGCGACAAGTGGCAGCTTCACGCATTCCCTCACCACGCTGGCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGttt                                                                  <  1:161435/149‑1 (MQ=255)
                                                                            cggggaTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCa                                                               <  2:337260/144‑1 (MQ=255)
                                                                              ggCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAcc                                                             <  2:252807/149‑1 (MQ=255)
                                                                                  ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                                         <  2:672629/117‑1 (MQ=255)
                                                                                  ttttCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                                         >  1:672629/1‑117 (MQ=255)
                                                                                           ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTc                                                          <  1:559147/139‑1 (MQ=255)
                                                                                           ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTc                                                          >  2:559147/1‑139 (MQ=255)
                                                                                           ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGc                                                >  2:35013/1‑149 (MQ=255)
                                                                                                gCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTaa                                           <  1:250359/149‑1 (MQ=255)
                                                                                                  gACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCGGAGCGGTAACa                                         >  1:7798/1‑149 (MQ=255)
                                                                                                   aCAAGTTGCAGCTTCACGCATTGACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCAGtgggct                                        <  2:86155/149‑6 (MQ=255)
                                                                                                     aaGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGc                                      <  2:514867/149‑1 (MQ=255)
                                                                                                              cctCACGCATTAACCCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTgcgc                             <  1:26641/147‑1 (MQ=255)
                                                                                                                tCACGCATTAACTCCAGAGTTTTTCCAGTTGATCCAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCga                           <  1:129089/149‑1 (MQ=255)
                                                                                                                tCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCga                           <  1:209550/149‑1 (MQ=255)
                                                                                                                tCACGCACTAACCACAGAGTGTGTCCCGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACACTCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGGCCGCTGCGCga                           <  1:435230/149‑1 (MQ=255)
                                                                                                                       ttAACGCCAGATTTTTTCCATTTGATACAGGCGACGGCTCTCTCCCTTCATGACATGGACAATCACATCGCCCAAATCCACTACAATCCAGTCGGCGCTGTCTTCACCTGCTACGCCGAGCGGTAACATGCCCGCTGCGCGAGACTCCt                    <  2:579165/149‑1 (MQ=255)
                                                                                                                               agagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACg            >  1:473210/1‑149 (MQ=255)
                                                                                                                                gagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGCCAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTACCAGGCCCGCTGCGCGAGACTCCTGccacacgt           >  1:174210/1‑142 (MQ=255)
                                                                                                                                gagTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGt           >  1:655956/1‑149 (MQ=255)
                                                                                                                                     tttCCAGTTGGTACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCa      <  1:517428/149‑1 (MQ=255)
                                                                                                                                      ttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAg     <  2:473210/149‑1 (MQ=255)
                                                                                                                                         cAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTCCGCAACGTGGTCAGCaa  >  2:136515/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                               
GGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAA  >  NC_000913_3_KO_pgi/668600‑668885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑