New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	3239675 =	38 (0.930)	5 (0.130)	5/272	4.9	11.4%	intergenic (+130/‑269)	alx/sstT	putative membrane‑bound redox modulator/sodium:serine/threonine symporter
	?	NC_000913_3_KO_pgi	3239699 =	41 (1.040)					intergenic (+154/‑245)	alx/sstT	putative membrane‑bound redox modulator/sodium:serine/threonine symporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCAT  >  NC_000913_3_KO_pgi/3239539‑3239820                                                                                                                                         |                                                                                                                                                   tGGGTACTTTTTGATCTGCCTAAGCCGTCGCCCCTTTCATTAACATCAGCACCGTTTTCTCCCCCTCCCTTCCAGGGAGAGGTTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                                        <  2:135943/149‑1 (MQ=25)    aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCTCCTCACCCTAACCCTCTCCCAAAACgg                                                                                                                                     >  1:584086/1‑149 (MQ=255)               tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                                                                                          >  1:466800/1‑149 (MQ=255)               tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                                                                                          >  1:382752/1‑149 (MQ=255)                   ccTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAc                                                                                                                      <  1:50484/149‑1 (MQ=255)                             gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttt                                                                                                            >  2:434393/1‑149 (MQ=255)                              tACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttt                                                                                                           <  2:572652/149‑1 (MQ=255)                              tACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttt                                                                                                           <  2:466800/149‑1 (MQ=255)                               aCCCTGTCATTAGTGTGGGTACGGTTTTCTCCGCCGCCCTTGCAGGTAGTGGGTCGGGTTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCTCTCTCCCTAACCTTTTTTTCACAGGGGGGGAGGGGACTGACCGAGTCCttttt                                                                                                          <  2:513991/149‑1 (MQ=255)                                 ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTg                                                                                                        >  2:72768/1‑149 (MQ=255)                                                  cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTGGTCATCAGTCTg                                                                                       >  1:308586/1‑149 (MQ=255)                                                   aCCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                                                                                      >  1:145808/1‑149 (MQ=255)                                                    ccGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGa                                                                                     >  2:329419/1‑149 (MQ=255)                                                              cctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGt                                                                           >  1:492887/1‑149 (MQ=255)                                                              cctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGt                                                                           >  1:344542/1‑149 (MQ=255)                                                               ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt                                                                          >  1:579584/1‑149 (MQ=255)                                                               ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt                                                                          >  1:163208/1‑149 (MQ=255)                                                                tctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt                                                                          >  2:2075/1‑148 (MQ=255)                                                                tctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt                                                                          <  1:2075/148‑1 (MQ=255)                                                                       tCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑t                                                                 <  2:163208/149‑1 (MQ=255)                                                                        ccAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑tt                                                                <  2:579584/149‑1 (MQ=255)                                                                           gggAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑ttttt                                                             <  1:72768/149‑1 (MQ=255)                                                                            ggAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTa                                                            <  2:145808/149‑1 (MQ=255)                                                                             gagaGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTACCAATTCTTGAAGCCGCACGTTGGCTTTA‑TTTTTAt                                                           <  1:175514/149‑1 (MQ=37)                                                                                  ggTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATg                                                          >  1:698853/1‑145 (MQ=255)                                                                                  ggTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATg                                                          <  2:698853/145‑1 (MQ=255)                                                                                            aGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTaa                                            >  1:252962/1‑149 (MQ=255)                                                                                              ggTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTAAcc                                          >  1:589281/1‑149 (MQ=255)                                                                                               gTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATTTTTTATGTCAAAGAAATGTAAcc                                          >  2:544733/1‑149 (MQ=255)                                                                                                 aaTTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCTTTTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCAGAAGTTTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTAACCAtt                                       <  2:661411/149‑1 (MQ=39)                                                                                                  aTTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGAAAGCCGCAAGAAGCCATTA‑TTTTTATGTAAAAGAAAAGTAAACATTa                                      >  1:134650/1‑149 (MQ=37)                                                                                                       tCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTAACCATTAAGttt                                 <  1:487822/149‑1 (MQ=255)                                                                                                           aCCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGCCCGAGTCCTTTTTTGAtgt                                                                                                    <  2:609946/79‑1 (MQ=255)                                                                                                           aCCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTAACCATTAAGTTTCaaa                             >  1:517404/1‑149 (MQ=255)                                                                                                           aCCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACAGAGTCCTTTTTTGAtgt                                                                                                    >  1:609946/1‑79 (MQ=255)                                                                                                                   tGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGAcc                     >  2:363238/1‑149 (MQ=255)                                                                                                                             cccTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTAACCATTCAGTTTCAAAATATGAACTCCCTTTaaa           >  2:534403/1‑149 (MQ=255)                                                                                                                                      tAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCAt  >  1:453338/1‑149 (MQ=255)                                                                                                                                         |                                                                                                                                                   TGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTA‑TTTTTATGTCAAAGAAATGTAACCATTAAGTTTCAAAATATGACCTCTCTTTAAAATCCAGCAT  >  NC_000913_3_KO_pgi/3239539‑3239820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.