New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 1096961 =59 (1.440)3 (0.110)
+GGGATGAGAAGCCCCGACCGGGGTTCGACAACTGGCGACAGCCAGTT
3/188 4.4 8.1% intergenic (+132/+604) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913_3_KO_pgi 1097062 = 44 (1.080)intergenic (+233/+503) ycdU/serX putative inner membrane protein/tRNA‑Ser
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CATTTTAATAAGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGC  >  NC_000913_3_KO_pgi/1096815‑1097107
                                                                                                                                                  |                                                                                                                                                  
cATTTTAATAAGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCAccc                                                                                                                                                  >  2:650544/1‑149 (MQ=255)
 aTTTTAATAAGTGAGGAAGTGATAGAAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCg                                                                                                                                                 >  2:123617/1‑149 (MQ=255)
   tttAATAAGTGAGGACGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGCCAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGgt                                                                                                                                               >  2:86908/1‑149 (MQ=255)
      aataaGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGgtgtg                                                                                                                                            <  2:157238/149‑1 (MQ=255)
      aataaGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACGTAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGgtgtg                                                                                                                                            <  2:541192/149‑1 (MQ=255)
        taaGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGCTTTCTCATCTCCCTAACAGACGTGGGCGGGGGCACCCGGtgtgtg                                                                                                                                          <  1:713806/149‑1 (MQ=255)
            tGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGgtgtgtaaaat                                                                                                                                      >  1:702767/1‑144 (MQ=255)
               ggAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCAGTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAAtata                                                                                                                                   >  1:66236/1‑149 (MQ=255)
                   gTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGTAATCTACGaa                                                                                                                               >  1:330014/1‑149 (MQ=255)
                    tGATAGGAAGTGACCCGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGaaa                                                                                                                              <  1:136141/149‑1 (MQ=255)
                          gAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAg                                                                                                                        >  2:388941/1‑149 (MQ=255)
                                 ccAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTAc                                                                                                                 >  2:113457/1‑149 (MQ=255)
                                                    tCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAACAGCCCGTACTTTCGTCCGAAGTcttctt                                                                                              >  1:338414/1‑149 (MQ=255)
                                                    tCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTcttctt                                                                                              <  1:16486/149‑1 (MQ=255)
                                                      tGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTa                                                                                            <  1:292274/149‑1 (MQ=255)
                                                                        tgggttgctgACCGAGTAACCGGAAAGTTGAGTTTCACGATTTCCCCTCGCCCTAACAGACGTGGGCTGGGGCACGCGGTGTGGGCAATATACTAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAggggg                                                                          <  2:507812/145‑1 (MQ=18)
                                                                         tGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggg                                                                         >  2:452463/1‑149 (MQ=255)
                                                                          gATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAggggggg                                                                        <  1:481652/149‑1 (MQ=255)
                                                                             tgttgACGTAGTAACCAGACAGTTGATGTGGCGCATTTCCCCGCGCTCAAACAGACAGGGGCGGGGGCCGCCGGTGTGGGCAATATACGAAAAAAAAGCCCGTACTTTGGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAtt                                                                     <  2:659780/149‑1 (MQ=17)
                                                                               ttgACTGAGTAACCAGGCAGTAGAGGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                   <  1:479395/149‑1 (MQ=255)
                                                                               ttgACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                   <  1:696673/149‑1 (MQ=255)
                                                                                 gACTGAGTAAGCAGACAGGTGATGGGCACGATTGCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGTAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACt                                                                 <  2:330014/149‑1 (MQ=21)
                                                                                 gACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACt                                                                 <  1:406508/149‑1 (MQ=255)
                                                                                    tGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgc                                                              >  1:316039/1‑149 (MQ=255)
                                                                                       ggaaCCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgcttc                                                           <  1:307538/147‑1 (MQ=255)
                                                                                           ccAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTtcgctc                                                       >  1:110748/1‑149 (MQ=255)
                                                                                            cAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCtctt                                                                                                 <  2:71368/106‑1 (MQ=255)
                                                                                            cAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCtctt                                                                                                 >  1:71368/1‑106 (MQ=255)
                                                                                            cAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTACATATGGCGGTGAGGGGGGGTTTGACTCGCTtcgctcg                                                      >  1:414888/1‑149 (MQ=255)
                                                                                            cAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTtcgctcg                                                      >  1:428624/1‑149 (MQ=255)
                                                                                                 aGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggg                                                                           >  2:508385/1‑123 (MQ=255)
                                                                                                 aGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggg                                                                           <  1:508385/123‑1 (MQ=255)
                                                                                                   ttGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCg                                               >  1:192175/1‑149 (MQ=255)
                                                                                                      aTGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAAtata                                                                                                                                   <  1:281559/62‑1 (MQ=255)
                                                                                                      aTGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAAtata                                                                                                                                   >  2:281559/1‑62 (MQ=255)
                                                                                                       tgttCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTGTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCa                                           <  1:475276/149‑1 (MQ=255)
                                                                                                         tgCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGc                                         >  1:521292/1‑149 (MQ=255)
                                                                                                           cACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGccc                                       >  2:104912/1‑149 (MQ=255)
                                                                                                           cACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGccc                                       >  1:577063/1‑149 (MQ=255)
                                                                                                                   ccccTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTg                               >  2:432545/1‑149 (MQ=255)
                                                                                                                    cccTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTc                                                                                                             >  2:285870/1‑70 (MQ=255)
                                                                                                                    cccTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTc                                                                                                             <  1:285870/70‑1 (MQ=255)
                                                                                                                      cTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGGACGTACATGCGTTCGTGAGCCATAGGAAATATGGCGGTGCTGGGGAGATTGACCCTCGTCACTCGCCCGGCGGGCAGCGCGCTCACTTCGt                            >  1:534506/1‑149 (MQ=12)
                                                                                                                      cTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTCCCTTCTTTCGAGATAGTACGTAAATATGGCGGTGACGGGGGGATTGACTCGCTTCTCGCGCCCCGCGGGCAGCCCGCTCACTGCGt                            >  1:534103/1‑149 (MQ=17)
                                                                                                                      cTCCCCCTAACAGCCGTGGGCGGGGGCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGt                            <  1:187083/149‑1 (MQ=255)
                                                                                                                        cGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTc                          <  2:521292/149‑1 (MQ=255)
                                                                                                                          cccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCAc                        <  1:113457/149‑1 (MQ=255)
                                                                                                                          cccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCAc                        <  2:110748/149‑1 (MQ=255)
                                                                                                                           ccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACg                       >  2:422145/1‑149 (MQ=255)
                                                                                                                              aaCAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGCCAGCCCGCTAACTGCGTTCACGGTc                    >  1:710241/1‑149 (MQ=255)
                                                                                                                                  gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGCGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc                >  1:685648/1‑149 (MQ=255)
                                                                                                                                     gTGCGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCaa             <  1:86908/149‑1 (MQ=38)
                                                                                                                                           ggggtCACCCGGTGTGGGCAATATACGAAAAAAAAGCCCGTACTTTCGTACCAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCt       <  1:611560/149‑1 (MQ=12)
                                                                                                                                           gggggCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                   <  2:312051/89‑1 (MQ=39)
                                                                                                                                           gggggCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                   >  1:312051/1‑89 (MQ=39)
                                                                                                                                            ggggCACCCGGTGTGTACAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTg      <  2:702767/149‑1 (MQ=21)
                                                                                                                                              ggCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTc    >  2:34069/1‑149 (MQ=32)
                                                                                                                                              ggCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTc    >  1:128478/1‑149 (MQ=32)
                                                                                                                                                cACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGc  <  2:128478/149‑1 (MQ=31)
                                                                                                                                                  |                                                                                                                                                  
CATTTTAATAAGTGAGGAAGTGATAGGAAGTGACCAGATAATACATATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGC  >  NC_000913_3_KO_pgi/1096815‑1097107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.