Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913_3_KO_pgi 4,233,757 +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC intergenic (‑524/‑583) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 42337570 (0.000)30 (1.090)
+ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC
24/190 0.2 100% intergenic (‑524/‑583) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
?NC_000913_3_KO_pgi 4233758 = 0 (0.000)intergenic (‑525/‑582) lysC/yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

GTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA  >  NC_000913_3_KO_pgi/4233612‑4233906
                                                                                                                                                  |                                                                                                                                                    
ctcaacattacgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGgt                                                                                                                                                    <  2:591754‑M2/3‑1 (MQ=255)
ctcaacatgccgctaacggcactaaaaccatcacatttttctctgactggcgctacaatcttccaaagtaacaactcgcaaaatcagaagagtattgctaatgaaaaacatcaatcccacgcagaccgctgcctggcaggcactacGgt                                                                                                                                                    <  2:372096‑M2/3‑1 (MQ=255)
          cgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGa                                                                                                                                          <  1:604363‑M2/13‑1 (MQ=255)
          cgctaacggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGa                                                                                                                                          <  1:258369‑M2/13‑1 (MQ=255)
                 ggcactaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcagaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCtt                                                                                                                                   <  1:645444‑M2/20‑1 (MQ=255)
                    actaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGa                                                                                                                                <  2:491361‑M2/23‑1 (MQ=255)
                     ctaaaaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGaa                                                                                                                               <  1:398316‑M2/24‑1 (MQ=255)
                         aaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTc                                                                                                                           >  1:367740‑M2/122‑149 (MQ=255)
                         aaccatcacatttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTc                                                                                                                           <  2:588187‑M2/28‑1 (MQ=255)
                             atcacttgttgcggtcactggcgccacaatcttccaaagtcacaattttcaaaataagaagagtattgctaatcaaaaacatccatcctacgcagaccgctgcctggcaggcactacGGTGTAGGCTGTAGCTGCTTCGAAGTTCCtat                                                                                                                       <  2:498693‑M2/32‑1 (MQ=255)
                                  atttttctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTTGGCTGGAGCTGCTTCGTATTTCCTATACttt                                                                                                                  >  2:476151‑M2/113‑149 (MQ=255)
                                     ttgctgtgactggcgctacaatcttccaaagtcacaattcttaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTTGAGCTGCTTCGAAGTTCCTATACTTTCTa                                                                                                               <  2:360092‑M2/40‑1 (MQ=255)
                                        ctgtgactggcgctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTagag                                                                                                            <  1:247373‑M2/43‑1 (MQ=255)
                                                   gctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACtt                                                                                                 >  1:315599‑M2/96‑149 (MQ=255)
                                                    ctacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTc                                                                                                <  2:367740‑M2/55‑1 (MQ=255)
                                                     tacaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCg                                                                                               <  2:701767‑M2/56‑1 (MQ=255)
                                                      acaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAgg                                                                                                                                               <  1:57255‑M2/8‑1 (MQ=255)
                                                      acaatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAgg                                                                                                                                               >  2:57255‑M2/93‑100 (MQ=255)
                                                        aatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATCCTTTCTAGAGCATAGGAACTTCGAAc                                                                                            >  2:135406‑M2/91‑149 (MQ=255)
                                                        aatcttccaaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAACAGGCACTTCGAAc                                                                                            >  2:74288‑M2/91‑149 (MQ=255)
                                                               caaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGt                                                                                     <  2:617544‑M2/66‑1 (MQ=255)
                                                               caaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTCCAGGt                                                                                     >  2:646780‑M2/84‑149 (MQ=255)
                                                                aaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTc                                                                                    >  1:553606‑M2/83‑149 (MQ=255)
                                                                aaagtcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTc                                                                                    >  2:6311‑M2/83‑149 (MQ=255)
                                                                   atcacaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTATGCTGGAGCTGCTCCGAAGTGCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGAc                                                                                 <  1:570060‑M2/70‑1 (MQ=255)
                                                                      acaattctcaaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctagcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGa                                                                              <  2:581751‑M2/73‑1 (MQ=255)
                                                                               aaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTCGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGaa                                                                     >  2:583473‑M2/68‑149 (MQ=255)
                                                                               aaaatcagaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGCGTTGACTGGAGCTGCGTCGAAGTCCCTATACTTTCTAGAGAATAAGAACTTCGAACTGCAGGTCGACGGATCCCCGGaa                                                                     >  1:642676‑M2/68‑149 (MQ=255)
                                                                                    tagaagagtattgctaacgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTCCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCcggaatgctc                                                                <  1:272371‑M2/87‑5 (MQ=255)
                                                                                      gaagagtattgctaatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCcccggaatgctcgc                                                              >  2:217764‑M2/61‑143 (MQ=255)
                                                                                                                                             actacggtgtagGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatg       >  1:679830‑M1/6‑88 (MQ=255)
                                                                                                                                               cacggtgtAGGCTGGCGCTGCTTCGAAGTTCCTAAACTTTCGAGAGAATAGGAACTTCGAACTGCAGGTCGACGTATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcaccgtcgatatgta     <  1:92785‑M1/146‑64 (MQ=255)
                                                                                                                                                  ggTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATgctcgaccaatggtctgattaaccgctataaagcgtggcgcggttaatcatcgtcgatatgtaggc  >  1:494852‑M1/1‑83 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTCAGTAGTTCAGCTAATAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGA  >  NC_000913_3_KO_pgi/4233612‑4233906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.