New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 1096988 =52 (1.270)6 (0.150) 6/276 4.6 10.5% intergenic (+159/+577) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913_3_KO_pgi 1097191 = NA (NA)intergenic (+362/+374) ycdU/serX putative inner membrane protein/tRNA‑Ser
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGT  >  NC_000913_3_KO_pgi/1097044‑1097306
                                                                                                                                                   |                                                                                                                   
tcgcttcgctCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGctct                                                                                                                    >  1:132912/1‑149 (MQ=34)
    ttcgctCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTcttctt                                                                                                                >  2:484917/1‑149 (MQ=35)
        ctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTaaa                                                                                                            <  2:132912/149‑1 (MQ=35)
        ctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTaaa                                                                                                            >  1:55374/1‑149 (MQ=35)
             ccTGCGGGCAGCCCGGTGCCTGCGTTGAGGGCCTGTCCAGCTGGTTGTCGCCAGCTGCCGGCCCGCGGTCGCGGCTTCTCATCCCCCCGCTGTGTGTCATCCATGTAAAATAAGCCCGTACTTTCGTACGAGCTTTTCTTTAAATATgg                                                                                                       <  2:66236/149‑1 (MQ=2)
                       gCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggg                                                                                             <  1:484917/149‑1 (MQ=35)
                         ccGCTCACTGCGTTGACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACCCAAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggg                                                                                           <  2:686550/149‑1 (MQ=255)
                            cTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATAAACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAt                                                                                        <  2:55374/149‑1 (MQ=17)
                               aCTGCGCGCACGGTCTGCCCAAGTGGCGGTCGCCTGTTGTCGACCCTCGGTGGGGGCTTCTGAGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                                     <  2:428624/149‑1 (MQ=11)
                                                                        gACCCCCGGTCCGGCCGTCTCATCCCCCCTGTGTGTGCAATACACGAAAGAAAAGCCCGTACGTTCGTACGAGCTCTTCTTTCAAGATGGCGGGGAGGGGAGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTAACTGCGttg                                            >  2:653712/1‑148 (MQ=11)
                                                                         gccccccGGCGGGGGGTCCCGTCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCa                                           <  1:650544/148‑1 (MQ=11)
                                                                            cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACgg                                        >  1:587005/1‑149 (MQ=32)
                                                                               ggTCGGGGCTTCTCAGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCt                                     <  1:388941/149‑1 (MQ=17)
                                                                                gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGt                                                                                                   >  2:378945/1‑86 (MQ=35)
                                                                                gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGt                                                                                                   <  1:378945/86‑1 (MQ=35)
                                                                                 tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGc                                                                  <  1:41845/118‑1 (MQ=32)
                                                                                 tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGc                                                                  >  2:41845/1‑118 (MQ=32)
                                                                                  cGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc                                  <  2:587005/149‑1 (MQ=32)
                                                                                  cGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc                                  <  1:291973/149‑1 (MQ=32)
                                                                                       cTTCTCATCCCCCCGGTGTGTGCAATACACGAGAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTCTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTACTGGCAGCCCGCTCACCGCGTTCACGGTCTGTCCAACt                             >  2:386514/1‑149 (MQ=11)
                                                                                             aTCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGcc                                                                      >  2:492471/1‑102 (MQ=33)
                                                                                                           tgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGaa         >  2:226434/1‑149 (MQ=32)
                                                                                                           tgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGaa         >  2:96091/1‑149 (MQ=32)
                                                                                                                  cacGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGTGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGt  <  1:341740/149‑1 (MQ=11)
                                                                                                                                                   |                                                                                                                   
TCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGT  >  NC_000913_3_KO_pgi/1097044‑1097306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.