New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi 1097007 =51 (1.250)8 (0.200) 7/276 4.2 13.8% intergenic (+178/+558) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913_3_KO_pgi 1097172 = NA (NA)intergenic (+343/+393) ycdU/serX putative inner membrane protein/tRNA‑Ser
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGT  >  NC_000913_3_KO_pgi/1097024‑1097306
                                                                                                                                                    |                                                                                                                                      
gCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGcc                                                                                                                                        <  1:123617/149‑1 (MQ=35)
         gggggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTc                                                                                                                               <  1:432545/149‑1 (MQ=35)
                    tcgcttcgctCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGctct                                                                                                                    >  1:132912/1‑149 (MQ=34)
                        ttcgctCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTcttctt                                                                                                                >  2:484917/1‑149 (MQ=35)
                            ctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTaaa                                                                                                            <  2:132912/149‑1 (MQ=35)
                            ctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTaaa                                                                                                            >  1:55374/1‑149 (MQ=35)
                                 ccTGCGGGCAGCCCGGTGCCTGCGTTGAGGGCCTGTCCAGCTGGTTGTCGCCAGCTGCCGGCCCGCGGTCGCGGCTTCTCATCCCCCCGCTGTGTGTCATCCATGTAAAATAAGCCCGTACTTTCGTACGAGCTTTTCTTTAAATATgg                                                                                                       <  2:66236/149‑1 (MQ=2)
                                           gCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggg                                                                                             <  1:484917/149‑1 (MQ=35)
                                             ccGCTCACTGCGTTGACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACCCAAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggg                                                                                           <  2:686550/149‑1 (MQ=255)
                                                cTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATAAACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAt                                                                                        <  2:55374/149‑1 (MQ=17)
                                                   aCTGCGCGCACGGTCTGCCCAAGTGGCGGTCGCCTGTTGTCGACCCTCGGTGGGGGCTTCTGAGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGa                                                                                     <  2:428624/149‑1 (MQ=11)
                                                                         cTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTc                                                                                                                               >  1:678130/1‑85 (MQ=39)
                                                                                            gACCCCCGGTCCGGCCGTCTCATCCCCCCTGTGTGTGCAATACACGAAAGAAAAGCCCGTACGTTCGTACGAGCTCTTCTTTCAAGATGGCGGGGAGGGGAGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTAACTGCGttg                                            >  2:653712/1‑148 (MQ=11)
                                                                                             gccccccGGCGGGGGGTCCCGTCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCa                                           <  1:650544/148‑1 (MQ=11)
                                                                                                cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACgg                                        >  1:587005/1‑149 (MQ=32)
                                                                                                   ggTCGGGGCTTCTCAGCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCt                                     <  1:388941/149‑1 (MQ=17)
                                                                                                    gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGt                                                                                                   >  2:378945/1‑86 (MQ=35)
                                                                                                    gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGt                                                                                                   <  1:378945/86‑1 (MQ=35)
                                                                                                     tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGc                                                                  <  1:41845/118‑1 (MQ=32)
                                                                                                     tCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGc                                                                  >  2:41845/1‑118 (MQ=32)
                                                                                                      cGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc                                  <  1:291973/149‑1 (MQ=32)
                                                                                                      cGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc                                  <  2:587005/149‑1 (MQ=32)
                                                                                                           cTTCTCATCCCCCCGGTGTGTGCAATACACGAGAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTCTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTACTGGCAGCCCGCTCACCGCGTTCACGGTCTGTCCAACt                             >  2:386514/1‑149 (MQ=11)
                                                                                                                 aTCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGcc                                                                      >  2:492471/1‑102 (MQ=33)
                                                                                                                               tgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGaa         >  2:226434/1‑149 (MQ=32)
                                                                                                                               tgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGaa         >  2:96091/1‑149 (MQ=32)
                                                                                                                                      cacGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGTGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGt  <  1:341740/149‑1 (MQ=11)
                                                                                                                                                    |                                                                                                                                      
GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGT  >  NC_000913_3_KO_pgi/1097024‑1097306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.