New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_KO_pgi	= 2662483	39 (0.770)	7 (0.210) +CTACAGTGAACAGAACCGTAGGTCGGATAAGGCGTTCACGCCGCATCCG	4/188	5.2	21.5%	noncoding (175/266 nt)	REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences	REP184 (repetitive extragenic palindromic) element; contains 6 REP sequences
	?	NC_000913_3_KO_pgi	= 2662583	NA (NA)					coding (741/741 nt)	trmJ	tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
Rejected: Coverage evenness skew score above cutoff.

CGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAAT  >  NC_000913_3_KO_pgi/2662335‑2662630                                                                                                                                                     |                                                                                                                                                     cGCCCTGGGGTTCTGTACACCGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGc                                                                                                                                                      <  1:90985/149‑1 (MQ=38)         ggTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAAGGCCTTATCCGACCTACGGTTCTGATCACTGTAGGCCTGATAAGACGCGTTACGCGTCGCGTCAGCCACCGGCTg                                                                                                                                              >  1:368349/1‑149 (MQ=11)              tGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGa                                                                                                                                         >  1:205183/1‑149 (MQ=35)               gTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCACAGGCTGTCGAAt                                                                                                                                        >  1:355443/1‑149 (MQ=16)                    cTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTGTGTTCACTGTAGGGCTGATAAGACGCATTTCCCGTCGCATCTGGCAACGGCTGTCAGATgcgcc                                                                                                                                   >  1:306222/1‑147 (MQ=11)                          gCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGACTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAAc                                                                                                                             >  2:89517/1‑149 (MQ=255)                                tAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTa                                                                                                                       <  1:698748/149‑1 (MQ=255)                                  aGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGTCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGcc                                                                                                                          <  2:682179/144‑1 (MQ=255)                                  aGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGcc                                                                                                                          >  1:682179/1‑144 (MQ=255)                                   gACGCATTACGCGTCGCATCAGGCAACGGCTATCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATcc                                                                                                                    >  1:694659/1‑149 (MQ=255)                                            cgcgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACgg                                                                                                           <  1:767449/149‑1 (MQ=255)                                            cgcgTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACgg                                                                                                           <  1:487525/149‑1 (MQ=255)                                            cgcgTCGCATCAGGCAACGGCTATCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACgg                                                                                                           <  2:694659/149‑1 (MQ=255)                                                    aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGCCGTTTACGCCATATCCAACCCACGGTTCTGGTc                                                                                                   >  2:49105/1‑149 (MQ=14)                                                       gtggaaCGGCTGGCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGACTTATCCGACCTACGGTTCTGTTCACt                                                                                                <  2:897010/145‑1 (MQ=255)                                                              ggCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGTTGGGGCGGGTACGCCTTATCGGAGCTCCGGTTCTGTTCAGTGTTGGcc                                                                                         >  1:901208/1‑149 (MQ=14)                                                              ggCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGGTCACTGTAGGcc                                                                                         >  1:145535/1‑149 (MQ=255)                                                                   tCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTCATa                                                                                    >  1:420910/1‑149 (MQ=255)                                                                     ggATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGAAGCGGCGTGAACGCCTTATACGACCTACGGATCGGTTCACCGTAGGCCTGATAAg                                                                                  >  1:248459/1‑149 (MQ=14)                                                                      gATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGa                                                                                 >  1:645267/1‑149 (MQ=255)                                                                       gtGCGGCGTGCACGCCTTATCCGGCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGAc                                                                                <  1:358816/148‑1 (MQ=255)                                                                        tGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACg                                                                               <  1:537914/149‑1 (MQ=255)                                                                          cggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCa                                                                             >  1:712663/1‑149 (MQ=255)                                                                           ggcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑t                                                                           <  1:111567/149‑1 (MQ=255)                                                                            gcgTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATGCGACGcgta                                                                           >  1:852677/1‑146 (MQ=255)                                                                                    gCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCg                                                                  >  2:745156/1‑149 (MQ=255)                                                                                     ccTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGc                                                                 <  2:712663/149‑1 (MQ=255)                                                                                       ttATACGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAGGGTTCTGTTCACTGTAGGCCTGATAAAACGCA‑TTACGCGTCGCAt                                                               >  2:762902/1‑149 (MQ=255)                                                                                                           ttCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCAGATGCGGCGGGAACCCCTTATCCGACCTACGATTCTGGTCACTGTAGGCCGGAGGAGACGCA‑TCACCCGCCTCATCCGCCGACGGCTGTTaacca                                           >  2:645523/1‑145 (MQ=11)                                                                                                            tCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCgg                                          <  1:745156/149‑1 (MQ=35)                                                                                                                tGTAGGCCTGATAAGACGCATTACGCGTCACAACAAGGAGCTGCTGTCTGATGCGCCGTGAACGACTTATCCGACCTACGGGTCTGTTCACTGTATGCCCCATGAGA‑GCACTTACGCGCCGCATCATGCGCCGGCGGTTATTCTGGCtt                                      >  2:874509/1‑149 (MQ=2)                                                                                                                  tAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGt                                    <  2:880189/149‑1 (MQ=40)                                                                                                                     gCCTGATAAGGCGCATTCCCCGTCGCATCCGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTAc                                 <  1:49105/149‑1 (MQ=255)                                                                                                                            aaGACGCGTTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGTCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTAtt                          <  1:353013/149‑1 (MQ=255)                                                                                                                               ccgttttACGCGGCGCGGCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGCCCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctg                       <  2:380912/144‑1 (MQ=17)                                                                                                                                  cATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTctactg                    <  1:863938/149‑1 (MQ=255)                                                                                                                                    ttACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTgctgct                  >  1:570280/1‑149 (MQ=255)                                                                                                                                         ggTCGCATAAGGCAACGGCTGTCGGATGCGGCGTTAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTATGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATa             <  2:720078/148‑1 (MQ=255)                                                                                                                                          gTCGCATCAGGAAACGGCTGTCGGATGCGGCGTGAACGCCTTCTTCGACCTACGGTTCTGTTAACTGTGGGCCTGATAAGCAGCA‑TTACGCGTCGGATATGGCGACGGCTGTTCTTCGGCCTTGTTAGCTTTAATGTGATGCCCAATac            >  2:732502/1‑148 (MQ=14)                                                                                                                                            cGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGaa          <  1:89517/149‑1 (MQ=255)                                                                                                                                               aTCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGATCAATAGCAGcc       >  2:91807/1‑149 (MQ=255)                                                                                                                                                tCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCa      <  1:16494/149‑1 (MQ=255)                                                                                                                                                  aGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGa    >  1:857109/1‑149 (MQ=255)                                                                                                                                                    gCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGTCAACGGCTGTTATTCGCGCTTGGTACCCTTATTCCGCTGCACAATAGGAGTCACAAt  >  2:289968/1‑149 (MQ=14)                                                                                                                                                     |                                                                                                                                                     CGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCA‑TTACGCGTCGCATCAGGCAACGGCTGTTATTCGGCCTTGTTACCTTTATTCTGCTGCTCAATAGAAGCCAGAAT  >  NC_000913_3_KO_pgi/2662335‑2662630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.