| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913_3_KO_pgi | 4,233,757 | +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC | intergenic (‑524/‑583) | lysC ← / → yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913_3_KO_pgi | = 4233757 | 0 (0.000) | 40 (1.160) +ATGAAAAACATCAATCCAACGCAGACCGCTGCCTGGCAGGCACTAC |
36/194 | 0.0 | 100% | intergenic (‑524/‑583) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein |
| ? | NC_000913_3_KO_pgi | 4233758 = | 0 (0.000) | intergenic (‑525/‑582) | lysC/yjbE | lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein | |||||
TAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCG > NC_000913_3_KO_pgi/4233629‑4233893 | taaATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaac < 1:328783‑M1/149‑21 (MQ=255) aaTGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGCCTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacac > 2:24809‑M1/1‑127 (MQ=255) ttCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagacc > 1:13817‑M1/1‑122 (MQ=255) aCTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcaaaccgct > 1:152018‑M1/1‑119 (MQ=255) tGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTTTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTAAAAATCAGAAGAGTATTGCTAatgaaaaaaataaatccaacgcagaccgcttc > 2:51824‑M1/1‑117 (MQ=255) aaGGGTTTACACTCAACATTACGCTAACGGCCCTCACCCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggca < 1:246692‑M1/149‑39 (MQ=255) aaGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTCTCGCCGatcaaaaacatcaatccaacgcagaccgctgcctggca < 1:440525‑M1/149‑39 (MQ=255) aaGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggca < 2:393466‑M1/149‑39 (MQ=255) acTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcaaaccgctgcctggcaggcactacgg > 2:706671‑M1/1‑101 (MQ=255) cTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggt > 1:3318‑M1/1‑100 (MQ=255) tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtt > 2:290509‑M1/1‑99 (MQ=255) tCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtg > 2:111414‑M1/1‑99 (MQ=255) tCAACATTACGCTAACGGCACTAAAACAATCAAATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggaactacggtg > 1:123557‑M1/1‑99 (MQ=255) cATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtagg < 1:341297‑M1/149‑55 (MQ=255) gCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggag > 1:788952‑M1/1‑89 (MQ=255) ggCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTTCTAatgaaaaacatcaatccaacgcagaccgctccctggcaggcactacggtgtaggctggagctgctt > 2:251545‑M1/1‑83 (MQ=255) aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCCCAAAATCAGAAGAGTATTGCTAaagaacaccatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcga < 1:870704‑M1/149‑70 (MQ=255) aCTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGACACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatacaaagcagaccgctgcctggcaggcactacggtgtagcctggacctgcttcga > 2:752742‑M1/1‑80 (MQ=255) cTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaa < 1:507993‑M1/149‑71 (MQ=255) tAAAACCATCACATTTTTCTGTGACTGCCGCTAACCCCACCTATAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacgttgtaggctggggctatttagaag > 2:153181‑M1/1‑78 (MQ=255) aaactcatcacATTTTCATGTGCCTGGCGCCACAATCTTCCAAAGTAACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaccacctgaagcctacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagt < 1:302922‑M1/144‑73 (MQ=255) aaCCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATCATCACAACAAGAAGAGTATTGGTAatgaagaacatcatgccggcgcagtccgctgcctggcaggcactacggtgtaggctggagctgctgcgaagttc < 1:178298‑M1/149‑75 (MQ=255) aTCACTTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATTAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtggagtctggagctgcttcgaagttcctat < 2:104850‑M1/149‑79 (MQ=255) tCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctata > 1:240365‑M1/1‑70 (MQ=255) cacaTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatac < 1:326553‑M1/149‑81 (MQ=255) aTTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctctacttt > 1:422953‑M1/1‑66 (MQ=255) ttttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttct < 1:517336‑M1/149‑86 (MQ=255) tttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcagtgacgtcggtgtaggctggagctgcttcgaagttcctatactttcta < 1:666642‑M1/149‑87 (MQ=255) ttCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctag < 2:672117‑M1/149‑88 (MQ=255) tgACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaat < 1:12056‑M1/149‑93 (MQ=255) aCTGGCGCTACTATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAacgaaaaccatcaatccaacgcagaccacggcctgccaggcactacgttgtaggctggagctgcttcgaagttcctatactttctagacaatag < 2:184603‑M1/149‑95 (MQ=255) cTGGCTCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatagg < 1:443820‑M1/149‑96 (MQ=255) cTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAat < 2:865619‑M1/56‑3 (MQ=255) cTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAat > 1:865619‑M1/1‑54 (MQ=255) tGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaatcgga > 2:471216‑M1/1‑53 (MQ=255) tGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctacagaatagga > 2:557340‑M1/1‑53 (MQ=255) cgcTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaact < 1:906968‑M1/149‑100 (MQ=255) cTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtagacgtgagctgtttcgaagttcctatactttctagagaataggaacttc < 1:128573‑M1/149‑102 (MQ=255) aaTCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggagcttcgaac > 1:123902‑M1/1‑44 (MQ=255) tCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactg > 1:601673‑M1/1‑42 (MQ=255) cTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgc < 1:111414‑M1/149‑109 (MQ=255) cAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggt > 2:217263‑M1/1‑37 (MQ=255) aaaGTCACAATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtc > 1:712743‑M1/1‑36 (MQ=255) cacaATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacgg < 2:805240‑M1/149‑119 (MQ=255) acaATTCTCAAAATCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacgga > 1:520294‑M1/1‑30 (MQ=255) aaTTCTCAAAATCAAAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggacctgcttccaagttcccatactttctagagaatagcaacttcgaactgcaggtcgacggatc > 2:696773‑M1/1‑28 (MQ=255) aaaaGCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaa < 2:769723‑M1/149‑129 (MQ=255) aaaTCAGAAGAGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaat > 1:678399‑M1/1‑20 (MQ=255) gaagaGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggcttgagctgcttcgaagttcctatactttctagataataggaacttcgaactgcaggtcgacggatccccggaatgctcga < 2:201893‑M1/149‑136 (MQ=255) aagaGTATTGCTAatgaaaaacatcaatccaacgcagaccgctgcctggcaggcactacggtgtaggctggagctgcttcgaagttcctatactttctagagaataggaacttcgaactgcaggtcgacggatccccggaatgctcgac > 1:127380‑M1/1‑13 (MQ=255) | TAAATGCTTCACTGCGCTAAGGGTTTACACTCAACATTACGCTAACGGCACTAAAACCATCACATTTTTCTGTGACTGGCGCTACAATCTTCCAAAGTCACAATTCTCAAAATCAGAAGAGTATTGCTAGGTGTAGGCTGGAGCTGCTTCGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCGAACTGCAGGTCGACGGATCCCCGGAATATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCG > NC_000913_3_KO_pgi/4233629‑4233893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |