Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_KO_pgi 1,978,503 Δ776 bp insB1insA insB1, insA

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_KO_pgi 1978503 1979278 776 48 [0] [0] 49 insB1–insA insB1,insA

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 19785020 (0.000)47 (0.980) 40/270 0.2 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913_3_KO_pgi 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein

AATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913_3_KO_pgi/1978358‑1978648
                                                                                                                                                |                                                                                                                                                  
aaggggtctTGAGACTGATTTAAAGGTTTTCCGAAATGGACGCAACTGTACTCGTAACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTTATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtata                                                                                                                                                <  2:703933‑M1/146‑5 (MQ=255)
 aTGTGTCTTTCCACTTATCTAATAATTTTCCTAAATCGACGCAACTGTACTTGTCACTACACGCATATACAACGGAGGGGTGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataa                                                                                                                                               <  2:223157‑M1/149‑6 (MQ=255)
     gTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtca                                                                                                                                           >  1:301377‑M1/1‑140 (MQ=255)
      tCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcat                                                                                                                                          <  1:71012‑M1/149‑11 (MQ=255)
      tCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcat                                                                                                                                          >  1:25739‑M1/1‑139 (MQ=255)
              cTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgt                                                                                                                                  <  2:853764‑M1/149‑19 (MQ=255)
                tATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttt                                                                                                                                <  2:746467‑M1/149‑21 (MQ=255)
                   cTAAGATTTTTCCTAAATCGACGCAATTGTACTCGTTACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                             <  1:562927‑M1/149‑24 (MQ=255)
                   cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                             <  1:483341‑M1/149‑24 (MQ=255)
                    tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGCTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttggttttggg                                                                                                                            >  1:859017‑M1/1‑125 (MQ=255)
                    tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttcgg                                                                                                                            >  1:417133‑M1/1‑125 (MQ=255)
                    tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtcttggg                                                                                                                            >  2:649694‑M1/1‑125 (MQ=255)
                    tAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGCGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatcctttttatttggg                                                                                                                            >  2:80307‑M1/1‑125 (MQ=255)
                     aaGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactt                                                                                                                                      >  2:83332‑M1/1‑124 (MQ=255)
                     aaGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactt                                                                                                                                      <  1:83332‑M1/138‑15 (MQ=255)
                     aaGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACAATGAATTACTTAACATAAATGtataagtcatacttttgtcttgggg                                                                                                                           >  2:800236‑M1/1‑124 (MQ=255)
                        aTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCCATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgta                                                                                                                        >  1:234126‑M1/1‑121 (MQ=255)
                        aTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgta                                                                                                                        <  2:13060‑M1/149‑29 (MQ=255)
                        aTTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTACGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttggatgta                                                                                                                        >  1:764515‑M1/1‑121 (MQ=255)
                               cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                                 >  2:89288‑M1/1‑114 (MQ=255)
                               cTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaat                                                                                                                 <  1:373048‑M1/149‑36 (MQ=255)
                                    tCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtt                                                                                                            >  1:488807‑M1/1‑109 (MQ=255)
                                           aaCTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgtgaaaaagt                                                                                                     >  1:546209‑M1/1‑102 (MQ=255)
                                                  cTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtgttgggtgtatttctatctgttaaaaagtttttcat                                                                                              >  2:859710‑M1/1‑95 (MQ=255)
                                                     gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctac                                                                                           <  1:649757‑M1/149‑58 (MQ=255)
                                                       cACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgc                                                                                         <  2:764515‑M1/149‑60 (MQ=255)
                                                        aCTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATGACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaaactgttaaaaagtttttcgctacgct                                                                                        >  2:21724‑M1/1‑89 (MQ=255)
                                                          tACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagttttt                                                                                                 >  1:874404‑M1/1‑87 (MQ=255)
                                                          tACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtgttgggtgtatttcaatctgttaaaaagttttt                                                                                                 <  2:874404‑M1/138‑52 (MQ=255)
                                                               gCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagc                                                                                 >  1:103935‑M1/1‑82 (MQ=255)
                                                                        aCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttttttttgttgtatttcaatctgttcaaaagtttttcgctacgctagcagacacaaatcac                                                                        >  2:569364‑M1/1‑73 (MQ=255)
                                                                         cGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaa                                                                       <  1:664586‑M1/149‑78 (MQ=255)
                                                                                 gggttcgatttTGAATAATGCGTGATTCAGATCACACAAAACAATCAATTAGTTAACATAAATGtataagtcatacttctggtttgggtgtatttcaatctgttaagaagttgttcgctacgctagcaagcaaaaatgaaacaggaata                                                               <  2:859017‑M1/144‑86 (MQ=255)
                                                                                  gCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataa                                                              >  2:862480‑M1/1‑63 (MQ=255)
                                                                                           ttCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaataatgaaacaggaataatcgaaatgg                                                     >  1:894592‑M1/1‑54 (MQ=255)
                                                                                                    gCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcg                                            <  1:361506‑M1/149‑105 (MQ=255)
                                                                                                    gCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcg                                            <  1:363336‑M1/149‑105 (MQ=255)
                                                                                                               aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaa                                 <  1:562642‑M1/149‑116 (MQ=255)
                                                                                                                tCACAAAAAACACTCAAGTAATTAACATAAAGGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagtaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaat                                <  2:181527‑M1/149‑117 (MQ=255)
                                                                                                                  acacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                              >  1:547753‑M1/1‑31 (MQ=255)
                                                                                                                    acaAAACACTCAATTACTTAACATAAATAtataagtaatacttttgtattgggtgtatttcaatcttttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgatattgggtgttgcgcacagccaacataacc                            >  2:104893‑M1/1‑29 (MQ=255)
                                                                                                                     caAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatctaaatgggatgttgcgcacagtcaaaataactc                           >  2:70960‑M1/1‑28 (MQ=255)
                                                                                                                      aaaaCACTCAATTACTTAACATAAATGtataagtcatccttttgttttgagtgtatttcaatctgttaaaaagtttttcgctaagcattcaagctaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactca                          <  1:757160‑M1/149‑123 (MQ=255)
                                                                                                                       aaaCACTCAATTACTTAACATAAATGtataagtcacacttgtgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcac                         <  1:244739‑M1/149‑124 (MQ=255)
                                                                                                                        aaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacccagcgccagagaaatgaaacaggaataattgaaatgggatgttgcgcacagtcaaaataactaccc                        >  1:662492‑M1/1‑25 (MQ=255)
                                                                                                                          cacTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgt                      >  1:258445‑M1/1‑23 (MQ=255)
                                                                                                                             tCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttcaaaagtttttcgctacgctagcaagcaaaactcaaacaggactaatccaaaagcgatgttgcgcacactcaaaataactcaccgtaaa                   >  2:31092‑M1/1‑20 (MQ=255)
                                                                                                                                              aTGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgctataaa  >  1:226447‑M1/1‑3 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
AATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAA  >  NC_000913_3_KO_pgi/1978358‑1978648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.