New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_KO_pgi = 22703944 (0.870)6 (0.120) 5/274 6.7 14.1% noncoding (1281/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913_3_KO_pgi = 4038805 31 (0.640)noncoding (1287/2905 nt) rrlA 23S ribosomal RNA of rrnA operon
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA  >  NC_000913_3_KO_pgi/4038658‑4038871
                                                                                                                                                   |                                                                  
cTAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAAAGGGTGAAAAGc                                                                   >  1:244613/1‑149 (MQ=255)
 tAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGcc                                                                  <  1:334191/149‑1 (MQ=40)
   aaCCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCg                                                                <  2:367986/149‑1 (MQ=255)
       aTGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCg                                                            >  1:69495/1‑149 (MQ=255)
       aTGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTACTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAATGATAAAGCGGGGGAAAAGcccgcccg                                                            >  2:881504/1‑149 (MQ=255)
         gCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAAGGATGACAACAGTAACGATGAAGGGGGGGAAAAGCACGCTCgac                                                          >  1:205415/1‑147 (MQ=14)
            ccGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCgat                                                       >  2:903510/1‑147 (MQ=255)
             cGAAGCTGCGGCAGCGACACTATGTGTTGTCGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGAATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCACGCCGGaa                                                      >  1:474791/1‑149 (MQ=255)
                 gCTGCGGCAGCGACACTATATGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGCAGGTATCAGAAGTGCGAATGCTGAGATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGAcc                                                  >  2:764035/1‑149 (MQ=255)
                     cGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCCTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAgg                                              >  2:791684/1‑149 (MQ=255)
                         aGCGACACTATGTGTTGGTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTc                                          <  1:744947/149‑1 (MQ=255)
                          gCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAGGGTGTGCTGTGAGGCGTGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCTCGCTCGCCGGAAGCCCAGGGGTTcc                                         >  2:215382/1‑149 (MQ=14)
                             acacTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGt                                      <  1:715393/149‑1 (MQ=38)
                             acacGATGTGTTGGTGGGTAGGGGAGCGTTCTGCAAGCCTGAGAAGGTGTGTTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTACCGATAAAGCGAGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGt                                      <  2:274954/149‑1 (MQ=11)
                             aacctttgtgATGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAACGCCCGCTCGCCGGAAGACCAAGGGTTCCTGt                                      <  2:721454/143‑1 (MQ=255)
                                  aTGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAAc                                 <  2:85532/149‑1 (MQ=35)
                                     ggttgttGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATACAGCGGATGACACGCCGGGTCGTCTGAACACCAAGGGTTCCTGTCCAACGtt                              >  2:412353/2‑149 (MQ=11)
                                       ttgttgGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTaa                            <  2:69495/149‑1 (MQ=32)
                                          ttgGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCgg                        >  2:709027/1‑149 (MQ=17)
                                           ggggTAGGGGAGCGCTCTGTAAGCCTGTGAAGGGGTGCTTTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACACACGTAACGATAAAGCGGGTGGAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCgg                        <  2:393316/148‑1 (MQ=11)
                                             ggTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGGGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCgggg                      <  1:75945/149‑1 (MQ=17)
                                                aGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAg                   >  1:870443/1‑149 (MQ=32)
                                                  gggAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGTGGGAGCAGAAGTGCGAATGCTGACATAGGTAACAATAACGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGCTCCTGCCCAACCTCAATCGGGGCAggc                 >  2:52745/1‑148 (MQ=11)
                                                   tgAGCGTTTGGTAAGCCTGTGAAGGTGTGCTGGGAGGGATGCGGGAGGTATCAGAAGTGGTAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCGCGCCGGAAGACCAAGGGTTCCTGTCCATCGTTAATCGGTGCAGGGt                <  2:215135/148‑1 (MQ=11)
                                                   ggAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGCGAGCACACTTTCATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGt                <  1:663158/149‑1 (MQ=11)
                                                      gCGTTCTGTAAGCCTTTGAAGGTGTGCTGTGTAGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGCCTTCTTGTCCAACGTTAATCGGGGCAGGGTGAg             <  2:56794/149‑1 (MQ=11)
                                                      gCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGTCGGGtaat             <  2:791448/149‑4 (MQ=12)
                                                          tCTGTAACCCTGTGAACGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGGCCAACAATCTGGCCCCAACGTTAATCGGGGCAGGGTGAGTCGa         <  2:647257/149‑1 (MQ=11)
                                                            tGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGAcc       >  1:743768/1‑149 (MQ=32)
                                                             gTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAgg                  >  2:504924/1‑137 (MQ=33)
                                                             gTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAgg                  <  1:504924/137‑1 (MQ=32)
                                                               aaGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCt    <  2:281147/149‑1 (MQ=32)
                                                               aaGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCt    >  1:809192/1‑149 (MQ=32)
                                                                 gCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTACTCGGGACAGGGTGAGTCGTCCCCtca  >  2:5452/1‑147 (MQ=255)
                                                                                                                                                   |                                                                  
CTAAACCATGCACCGAAGCTGCGGCAGCGACACTATGTGTTGTTGGGTAGGGGAGCGTTCTGTAAGCCTGTGAAGGTGTGCTGTGAGGCATGCTGGAGGTATCAGAAGTGCGAATGCTGACATAAGTAACGATAAAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAA  >  NC_000913_3_KO_pgi/4038658‑4038871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.