Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_KO_pgi 668,742 C→G E99Q (GAA→CAA)  rsfS ← ribosomal silencing factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_KO_pgi668,7420CG98.0% 148.6 / ‑3.3 51E99Q (GAA→CAA) rsfSribosomal silencing factor
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (22/28);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01

GCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATG  >  NC_000913_3_KO_pgi/668597‑668887
                                                                                                                                                 |                                                                                                                                                 
gccggAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATa                                                                                                                                                >  1:824628/1‑149 (MQ=255)
     aatgtgaaTCAGCTCGAAGTGCATATCTTGCGGAAAACGACGCAGGTGCTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGc                                                                                                                                           <  2:663880/143‑1 (MQ=255)
        ttCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGAc                                                                                                                                        >  2:842767/1‑149 (MQ=255)
          cAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAAGCGGTTTGTACCCAGTCCGGCGTTTTCGTTCCCACGGCGACAAGTTTCAGCTTCACGCATTAACTCCCGAGTTTTTCCAGTTGATACAGGCGACgg                                                                                                                                      >  1:729527/1‑149 (MQ=255)
                 cTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCtt                                                                                                                               >  1:578726/1‑149 (MQ=255)
                  tggcaGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTc                                                                                                                              <  2:464300/145‑1 (MQ=255)
                    gAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCt                                                                                                                            >  1:261948/1‑149 (MQ=255)
                            tatCTTTCGGTAAACGACGCAGGTGCTCGGTAAATCCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCGCTCTTCCTGCATGACa                                                                                                                    <  2:831776/149‑1 (MQ=255)
                             aacTTTCGGAAAACGACGCAGGTGCCCGGTGAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTGCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACAt                                                                                                                   <  2:54474/147‑1 (MQ=255)
                              tCTTTCAGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATg                                                                                                                  <  2:770979/149‑1 (MQ=255)
                                tttCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGa                                                                                                                >  1:565300/1‑149 (MQ=255)
                                    ggAAAACGACGAAGGTATTCGTGAACACCGGTTGTTACCCAGGCCGGCTTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCTCGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTGTTCCTGTATGACATGGACAAt                                                                                                            <  2:832840/149‑1 (MQ=255)
                                    agAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTGCCCAGTCCGGCAGTTTCGTTCCCAGGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGAGACAGGCGACGGCTCTCTTCCTGCATGACATGGACAAt                                                                                                            <  2:432484/148‑1 (MQ=255)
                                        aaCGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCGGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATcaca                                                                                                        >  1:316802/1‑149 (MQ=255)
                                             cgCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTAGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGcc                                                                                                   <  2:830518/149‑1 (MQ=255)
                                               tcggTACTTGGGAATACCGGTCTGTACCCAGTCCTGCCTTTTGGTTGTCACCGCGATAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATCAAGGCGACTGCTCCCTTCCTGCATGACATGGACAATCACACCGCCCa                                                                                                 <  2:11364/147‑1 (MQ=255)
                                                     cTCGGTAAAAGCGGTTTGTACCCAGTCCGGCATCTTCGTCCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTGTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCa                                                                                           <  2:887786/149‑1 (MQ=255)
                                                      tCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAGGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCACATCCAc                                                                                          >  2:881185/1‑149 (MQ=255)
                                                      tCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc                                                                                          >  1:459699/1‑149 (MQ=255)
                                                      tCGGTAAAACCGGTTTGTACCCAGTACGGCATTTTCGTTCAAACGGCGACAAGATGCAGCTTCACGCATTAACTCCAGAGTTTTTCCCGCTGCTACAGGCGACGGCTCTCTTCTGGCATGACATAGGTACTCACCTCGCCCAACTacac                                                                                          >  2:714132/1‑149 (MQ=255)
                                                        ggTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGa                                                                                        <  2:689626/149‑1 (MQ=255)
                                                                cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGa                                                                                                                                         <  2:550675/92‑1 (MQ=255)
                                                                cGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGa                                                                                                                                         >  1:550675/1‑92 (MQ=255)
                                                                 ggTTTCTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGt                                                                               <  2:216420/149‑1 (MQ=255)
                                                                        aCCCAGTCCGGCATTTTCGTTCCCACGGCGCCAAGTCGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCt                                                                        >  1:713857/1‑149 (MQ=255)
                                                                         cccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTCACTACAGAGGGTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAACAACATCGCCCAAATCCACGACAATCCAGTCGGCGCTg                                                                       >  2:828391/1‑149 (MQ=255)
                                                                          ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc                                                                                          <  2:116191/129‑1 (MQ=255)
                                                                          ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCAc                                                                                          >  1:116191/1‑129 (MQ=255)
                                                                          ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATc                                                                                   <  1:721261/136‑1 (MQ=255)
                                                                          ccAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATc                                                                                   >  2:721261/1‑136 (MQ=255)
                                                                           cgtgccGGCATTTTCGTTCCCGCCGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATAAAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGtt                                                                     <  2:237030/145‑1 (MQ=255)
                                                                              tCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTc                                                                  <  1:840709/149‑1 (MQ=255)
                                                                                cGGCGTCTTCGTTCCCACGGGGACAAGTTGCAGCTTCACGCATTAACTCCAGGGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACCATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAc                                                                <  2:469245/149‑1 (MQ=255)
                                                                                cGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGCCATGGACAATCCCATCGCCCAAATCCACGACAATCCAGGCGGCGCTGTTGTCAc                                                                >  1:16094/1‑149 (MQ=255)
                                                                                 ggCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCAcc                                                               >  1:712142/1‑149 (MQ=255)
                                                                                    aTTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGCCAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTc                                                            >  1:850214/1‑149 (MQ=255)
                                                                                             cccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTCCACCTTCTACGCCGAg                                                   <  1:789471/149‑1 (MQ=255)
                                                                                              ccACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGc                                                  <  1:842767/149‑1 (MQ=255)
                                                                                                 cGGTGACAAGTTGCAGCTTCACGCATTAGCTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGt                                               <  2:294521/149‑1 (MQ=255)
                                                                                                 cGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGt                                               >  1:491521/1‑149 (MQ=255)
                                                                                                       cAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGCCGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCCCTGTTTTCACCTTCTACGCCGAGCGGTCACAgg                                         >  1:95694/1‑149 (MQ=255)
                                                                                                           ttGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTACTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCg                                     <  2:2240/149‑1 (MQ=255)
                                                                                                           ttGCAGCTTCACGCATTAACTCAAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCACATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCg                                     <  2:2225/149‑1 (MQ=255)
                                                                                                                 cgtaaCGCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTgcgc                               <  2:729527/145‑1 (MQ=255)
                                                                                                                       gCATTAACTCCAGAGTTTTTCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGCCt                         >  1:40261/1‑149 (MQ=255)
                                                                                                                                        tttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCa        <  1:782289/149‑1 (MQ=255)
                                                                                                                                        tttCAAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCa        <  1:782025/149‑1 (MQ=255)
                                                                                                                                         ttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTg                                                                       <  1:838931/85‑1 (MQ=255)
                                                                                                                                         ttCCAGTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTg                                                                       >  2:838931/1‑85 (MQ=255)
                                                                                                                                             aGTTGATACAGGCGACGGCTCCCTTCCTGCATGACATGGACAACCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAAt   <  1:828836/149‑1 (MQ=255)
                                                                                                                                              gTTGATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATg  <  2:448537/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCCGGAATTTCAATCAGCTCGAAGGGCATATCTTTCGGAAAACGACGCAGGTACTCGGTAAAACCGGTTTGTACCCAGTCCGGCATTTTCGTTCCCACGGCGACAAGTTGCAGCTTCACGCATTAACTCCAGAGTTTTTCCAGTTCATACAGGCGACGGCTCTCTTCCTGCATGACATGGACAATCACATCGCCCAAATCCACGACAATCCAGTCGGCGCTGTTTTCACCTTCTACGCCGAGCGGTAACAGGCCCGCTGCGCGAGACTCCTGCACAACGTGGTCAGCAATG  >  NC_000913_3_KO_pgi/668597‑668887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑