Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,234,667 Δ1 bp intergenic (‑1434/‑499) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,234,6660A.99.0% 959.4 / 4.1 208intergenic (‑1433/‑500)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base . (105/99);  total (107/101)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.61e-01

Only 100 of 220 total aligned reads displayed.

ACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGT  >  NC_000913_3_pae_pgi/4234567‑4234761
                                                                                                   |                                                                                               
aCGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGATGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGa                                                                                                 >  1:5239811/1‑100 (MQ=255)
 cGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGat                                                                                                <  2:4141966/100‑2 (MQ=255)
  gagaTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGat                                                                                                <  2:4220316/99‑2 (MQ=255)
   agaTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGTGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCGTGCAGTGatca                                                                                              >  2:1362978/1‑97 (MQ=255)
   agaTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatca                                                                                              >  1:4009807/1‑97 (MQ=255)
   agaTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatca                                                                                              >  2:1485548/1‑97 (MQ=255)
      tgtgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcg                                                                                          >  2:4951592/1‑100 (MQ=255)
      tgtgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcg                                                                                          >  1:5258885/1‑100 (MQ=255)
        tgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgtc                                                                                        >  1:1116553/1‑100 (MQ=255)
          ggCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgtcg                                                                                       <  2:2479480/99‑1 (MQ=255)
           gCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGat                                                                                     <  1:3404780/100‑1 (MQ=255)
           gCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGa                                                                                      >  1:4457374/1‑99 (MQ=255)
            cATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGata                                                                                    <  2:1848120/100‑1 (MQ=255)
            cATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGata                                                                                    <  2:2904916/100‑1 (MQ=255)
            cATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGata                                                                                    >  1:1366986/1‑100 (MQ=255)
                nccTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATg                                                                                  >  1:723420/2‑98 (MQ=255)
                gCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATg                                                                                  >  1:2893785/1‑98 (MQ=255)
                  ctctGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAgg                                                                              <  1:3164603/100‑1 (MQ=255)
                    ctGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGcc                                                                            >  1:989466/1‑100 (MQ=255)
                    ctGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGcc                                                                            >  1:3640413/1‑100 (MQ=255)
                    ctGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGcc                                                                            >  2:3606594/1‑100 (MQ=255)
                     tGTAAAACTGGCAGAGCTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGACGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGc                                                                             >  2:231389/1‑98 (MQ=255)
                     tGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCg                                                                           >  1:3550312/1‑100 (MQ=255)
                      gTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAACGTCGATATGTAGGCCg                                                                           >  2:2571417/1‑99 (MQ=255)
                       tAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGcc                                                                            <  2:1218134/97‑1 (MQ=255)
                       tAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCgg                                                                          <  2:2648627/99‑1 (MQ=255)
                       tAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGa                                                                         >  1:2808734/1‑100 (MQ=255)
                       tAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGa                                                                         <  1:4499872/100‑1 (MQ=255)
                       tAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGa                                                                         <  2:11556/100‑1 (MQ=255)
                        aaaaCTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCCTGCAGTGATCATCGTCGATATGTAGGCCGGa                                                                         >  1:2120099/1‑99 (MQ=255)
                         aaaCTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGAtg                                                                       <  1:891488/100‑2 (MQ=255)
                         aaaCTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATa                                                                       <  1:1557737/100‑1 (MQ=255)
                         aaaCTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCGTGCAGCGa                                                                                                 >  2:2125405/1‑75 (MQ=255)
                            cTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAgg                                                                    <  1:3300055/100‑1 (MQ=255)
                               gCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCg                                                                  <  2:1875478/99‑1 (MQ=255)
                               gCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCg                                                                  <  2:2721160/99‑1 (MQ=255)
                               gCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCg                                                                  <  2:82712/99‑1 (MQ=255)
                               gCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGt                                                                 >  1:4801353/1‑100 (MQ=255)
                                cAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                >  1:1833288/1‑100 (MQ=255)
                                cAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCCTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCg                                                                  >  2:1566070/1‑98 (MQ=255)
                                 agagATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGtt                                                                >  1:1186084/1‑99 (MQ=255)
                                   agaTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAc                                                             >  1:1444063/1‑100 (MQ=255)
                                     aTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                           <  1:784308/100‑1 (MQ=255)
                                      ttAGAGGCGTAAATCCCCTAGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                          <  2:3087567/100‑1 (MQ=255)
                                        agagGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgccg                                                         <  2:3962176/99‑1 (MQ=255)
                                        agagGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGGCGAACTGTAGGCCGGATCAGGCGGTCAcgc                                                           >  2:3920746/1‑97 (MQ=255)
                                         gagGCGTAAATCCCCTGGCCACCCCTAGAATAGATGCATTGAAGAGGCGCCGGCAGCGATCATCGTCGCAATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                       >  2:66310/1‑100 (MQ=37)
                                          agGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCa                                                       <  2:4463480/99‑1 (MQ=255)
                                          agGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCAt                                                      >  2:1632101/1‑100 (MQ=255)
                                           ggCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                     >  1:4709156/1‑100 (MQ=255)
                                           ggCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATc                                                     >  1:1630193/1‑100 (MQ=255)
                                           ggCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATAAGTAGGCCGGATAAGGCGTTCACGCCGCCTc                                                     >  1:4880891/1‑100 (MQ=39)
                                              gTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                  <  1:4139135/100‑1 (MQ=255)
                                               tAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGCCGCTTGCAGTGATCACCCTCGATATGTAGGCCGGCTACGGCGTTCAcgcc                                                          >  2:3643083/1‑91 (MQ=255)
                                                 aaTCCCCTGGCCACCCCTAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                <  2:4794003/99‑1 (MQ=255)
                                                 aaTCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                               <  1:2907471/100‑1 (MQ=255)
                                                     cccTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                           >  1:1553202/1‑100 (MQ=255)
                                                       cTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATg                                         <  2:3266714/100‑1 (MQ=255)
                                                          gCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                       <  1:1270867/99‑1 (MQ=255)
                                                          gCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                       <  1:3126152/99‑1 (MQ=255)
                                                           ccACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGCTCATCGTCGCTATGTAGGCCGGATACGGCGTTCACGCCGCATCCGGCACCCGATGCCt                                      >  2:3456484/1‑99 (MQ=37)
                                                            cACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                    >  2:2309213/1‑100 (MQ=255)
                                                              ccccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAAGCTCGTCGATCTGTAGGCCGGGCAAGGCGATCACGCAGCCTCCGGCAACCCATGCCTGacg                                  >  2:4865035/1‑98 (MQ=255)
                                                              ccccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                  >  1:3760415/1‑100 (MQ=255)
                                                               cccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATg                                  <  1:2194943/99‑1 (MQ=255)
                                                               cccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                 >  1:3654216/1‑100 (MQ=255)
                                                                ccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                 >  1:3980661/1‑99 (MQ=255)
                                                                ccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                 >  1:1957667/1‑99 (MQ=255)
                                                                      aTAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCgg                          <  1:1720575/100‑1 (MQ=255)
                                                                       tAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGt                         <  2:2026282/100‑1 (MQ=255)
                                                                          aTGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcg                       <  2:5100541/99‑1 (MQ=255)
                                                                           tGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgcg                     <  1:1927866/100‑1 (MQ=255)
                                                                             cATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                   <  1:1706882/100‑1 (MQ=255)
                                                                             cATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                   >  1:3467634/1‑100 (MQ=255)
                                                                             cATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                   >  1:1079751/1‑100 (MQ=255)
                                                                             cATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                   <  1:2582084/100‑1 (MQ=255)
                                                                             cATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTAACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                   >  2:5056251/1‑100 (MQ=255)
                                                                                 gAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt               >  2:3778464/1‑100 (MQ=255)
                                                                                  aaGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATc              <  1:4864798/100‑1 (MQ=255)
                                                                                  aaGAGGCGCTTGCAGGGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgcg                     <  1:1928563/93‑1 (MQ=255)
                                                                                   agagGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt               >  2:808740/1‑98 (MQ=255)
                                                                                     agGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAgg           <  2:742804/100‑1 (MQ=255)
                                                                                     agGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAgg           <  2:4049785/100‑1 (MQ=255)
                                                                                     agGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg            <  2:4741229/99‑1 (MQ=255)
                                                                                       gcgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc         <  1:4128584/100‑1 (MQ=255)
                                                                                       gcgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc          <  2:4514115/99‑1 (MQ=255)
                                                                                       gcgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGGc          <  2:1917/99‑1 (MQ=18)
                                                                                        cgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc         >  2:898664/1‑99 (MQ=255)
                                                                                        cgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc         >  1:3232783/1‑99 (MQ=255)
                                                                                        cgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCCTATCAGGcc         >  2:5241440/1‑99 (MQ=255)
                                                                                         gcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATAAGACGCGACCGCGTCGCATCAGGcatc       <  1:1201283/100‑4 (MQ=18)
                                                                                         gcTGGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc          <  2:5213938/97‑1 (MQ=255)
                                                                                          cTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc      <  1:1875211/100‑1 (MQ=255)
                                                                                             gCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa     <  2:3022474/98‑1 (MQ=255)
                                                                                             gCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg   >  1:2412289/1‑100 (MQ=255)
                                                                                             gCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg    <  2:2356681/99‑1 (MQ=255)
                                                                                             gCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg    <  2:1508162/99‑1 (MQ=255)
                                                                                              cAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt  >  1:5133690/1‑100 (MQ=255)
                                                                                              cAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt  >  1:2664987/1‑100 (MQ=255)
                                                                                              cAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt  >  2:1310918/1‑100 (MQ=255)
                                                                                                   |                                                                                               
ACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGT  >  NC_000913_3_pae_pgi/4234567‑4234761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: