Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	4,234,667	Δ1 bp	intergenic (‑1434/‑499)	lysC ← / → yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	4,234,666	0	A	.	97.8%	196.1 / ‑3.2	46	intergenic (‑1433/‑500)	lysC/yjbE	lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (22/23);  minor base G (1/0);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAT  >  NC_000913_3_pae_pgi/4234528‑4234800                                                                                                                                           |                                                                                                                                       cGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGa                                                                                                                             <  1:688513/149‑1 (MQ=255)  gCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGat                                                                                                                            >  1:37097/1‑149 (MQ=255)    aGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGgtat                                                                                                                          <  2:4765/149‑1 (MQ=255)    aGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGatat                                                                                                                          >  1:662325/1‑149 (MQ=255)    aGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGatat                                                                                                                          >  1:660701/1‑149 (MQ=255)     gTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGCGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCCGCGatatt                                                                                                                         >  2:495371/1‑148 (MQ=255)      tAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCCATATGt                                                                                                                        >  1:677309/1‑149 (MQ=255)                            tttATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                  <  1:556007/149‑1 (MQ=255)                                            gaTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGa                                                                                  <  1:31489/149‑1 (MQ=255)                                                  ggCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTCCAG‑GGATCATCGTCGATATGTAGCCCGGGTAAGGCGTGCACGCCGCCTCCGGCAACCGATGCCta                                                                            >  2:427253/1‑148 (MQ=255)                                                    cATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt                                                                          <  1:588588/149‑1 (MQ=255)                                                    cATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt                                                                          <  2:37097/149‑1 (MQ=255)                                                        gCCTCTGTAAGACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGa                                                                      >  1:675015/1‑149 (MQ=255)                                                          ctctGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcg                                                                    >  1:633664/1‑149 (MQ=255)                                                           tctGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCCCTTGCACTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCTCGCCGCATCCGGCACCCTATGCCTGATGCTAcgc                                                                   >  2:596257/1‑149 (MQ=38)                                                            ctGTAAAACTGGCTGAGATTAGAGGCGTAAATCCCCTGGCCACTCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg                                                                  <  2:533421/149‑1 (MQ=255)                                                                   acgatcAGAGATTAGAGCCGTAAATCCCCTGTCCACGCCGATAATAGATTCATTGAAGAGTCGCTTGCAGTG‑AGCATCGTCTATATTTAGTCCGGATAAGGCGTTCACGCCGCATCCGACAAATGATGCCTGATTCGACGCTGTCGCGt                                                           <  2:573641/144‑1 (MQ=255)                                                                    cTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATACGTAGGCCGGATAAGGAGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                                                          <  1:512019/149‑1 (MQ=255)                                                                     tGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCt                                                         >  1:35977/1‑149 (MQ=255)                                                                          gagaTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATCGAAGCGGCGATTGCAGTG‑ATCATCGGCGACATGTAGCCCGGATATGCCGTTATTGCCGGCTCGAGCATACGAATCCTGATGCGCCGCGGTAGCGGCCTATCa                                                    >  2:326311/1‑149 (MQ=255)                                                                             attctatgcgcAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGc                                                 <  1:643457/142‑1 (MQ=37)                                                                                 gatgCGTAAATCCTCTGTCCACCACGAGAATAGATGCATGGAAGAGGCGCGTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAc                                             <  1:80228/146‑1 (MQ=25)                                                                                          aTCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATg                                    <  2:633664/149‑1 (MQ=255)                                                                                             cccTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCTACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCg                                 >  1:595659/1‑149 (MQ=255)                                                                                              ccTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                                >  1:606906/1‑149 (MQ=255)                                                                                                tgcccACCGCGAGAACAGATGCATAGAAGAGGCGCTTGGAGTG‑ATCATCGTCGATATGTGGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGata                              <  2:373733/146‑1 (MQ=25)                                                                                                  gCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt                                                                          <  1:386790/103‑1 (MQ=255)                                                                                                  gCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGAt                                                                          >  2:386790/1‑103 (MQ=255)                                                                                                       cccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                       >  1:59779/1‑149 (MQ=255)                                                                                                       cccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACAt                       >  1:522910/1‑149 (MQ=255)                                                                                                        ccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCTACGCGGTCGCGTCTTCTCAGGCCTACAGGTCGATGCCCATCTGTACATc                      >  2:359743/1‑149 (MQ=37)                                                                                                          gagaATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGt                    >  2:625195/1‑149 (MQ=255)                                                                                                            gaATAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAt                  <  2:409725/149‑1 (MQ=255)                                                                                                              aTAGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                <  1:661552/149‑1 (MQ=255)                                                                                                                aGATGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCgg              <  2:606906/149‑1 (MQ=255)                                                                                                                  aTGCATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCa            >  2:467148/1‑149 (MQ=255)                                                                                                                    gCAATGAAGAGGCGCTTGCAGTG‑ATGATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTAACGGCTTATGCCGATATGTACATCGTATTCGGCAAt          <  2:150242/149‑1 (MQ=25)                                                                                                                     cATTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt         <  2:522910/149‑1 (MQ=255)                                                                                                                      aTTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt         >  2:343548/1‑148 (MQ=255)                                                                                                                      aTTGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt         <  1:343548/148‑1 (MQ=255)                                                                                                                       ttGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaa       >  1:356236/1‑149 (MQ=255)                                                                                                                        tGAAGAGGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCATGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt      <  1:596257/149‑1 (MQ=255)                                                                                                                           agagGCGCTTGCAGTG‑ATCATCGTCGATATGTAAGCCGGATAAGGCGTTCACGCAGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGGCGATGCCGATATGTCAATCGTATTCGGCACTTatagaa   >  2:582001/1‑144 (MQ=25)                                                                                                                            gagGCGCTTGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAt  <  1:470516/149‑1 (MQ=255)                                                                                                                                   ttGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                     <  1:571221/75‑1 (MQ=255)                                                                                                                                   ttGCAGTG‑ATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                     >  2:571221/1‑75 (MQ=255)                                                                                                                                           |                                                                                                                                       CGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACAT  >  NC_000913_3_pae_pgi/4234528‑4234800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑