Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi526,7960CG27.3% 130.6 / 47.7 66A1179G (GCG→GGG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base C (23/25);  new base G (11/7);  total (34/32)
Fisher's exact test for biased strand distribution p-value = 4.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTAT  >  NC_000913_3_pae_pgi/526649‑526944
                                                                                                                                                       |                                                                                                                                                    
ttGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGc‑‑gcg                                                                                                                                                     <  1:247063/149‑1 (MQ=255)
 tGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGG‑‑GCgg                                                                                                                                                    >  1:395992/1‑149 (MQ=255)
   cgcAGGGCGGGCGGACGGTGGGGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCCTATCAGTATAGACGGCAATACGGCGTGGAGC‑‑GCGGaa                                                                                                                                                  <  2:325256/149‑1 (MQ=17)
      aGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAgtat                                                                                                                                               >  1:688512/1‑149 (MQ=255)
        ggcgggcggACGGTGGAGCAACCGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATatga                                                                                                                                             <  2:395992/148‑1 (MQ=255)
         gCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTatgat                                                                                                                                            <  1:109210/149‑1 (MQ=255)
               tGACGGTGGGGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATgg                                                                                                                                      <  2:245612/149‑1 (MQ=255)
                gACGGTGGCGCAACTGGCCAGACAGGTGGAGCCGGAATACCCACCGGCGCGAAAAGCTCATCTTTATCTCTGCGACCACCGGGGTCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATggg                                                                                                                                     <  1:162991/149‑1 (MQ=11)
                 aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATgggg                                                                                                                                    >  2:644825/1‑149 (MQ=255)
                 aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATgggg                                                                                                                                    >  1:321590/1‑149 (MQ=255)
                 aCGGGGGAGCCACTGGCCAGACAGGGGGAGGCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCGCTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGTAGGCGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATgggg                                                                                                                                    <  2:432612/149‑1 (MQ=17)
                  cGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATggggg                                                                                                                                   >  1:385533/1‑148 (MQ=255)
                   ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATTGGGCa                                                                                                                                  >  1:204579/1‑149 (MQ=255)
                     tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAAc                                                                                                                                <  1:450918/149‑1 (MQ=255)
                       gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCa                                                                                                                              >  1:259537/1‑149 (MQ=255)
                                gCCAGAGAGGTGGAGCCGGAATACACACCGGCGCGAAATGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                     <  2:23921/149‑1 (MQ=255)
                                gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGGGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                     >  1:601389/1‑149 (MQ=11)
                                gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                     >  1:550118/1‑149 (MQ=255)
                                gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGAAGGCAATACGGCGTGGCGG‑‑GGGGAGGAGAAGGAATGGGGCAGTCAGGTTAAGga                                                                                                                     >  2:480002/1‑149 (MQ=11)
                                 ccAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTGTATCACTGCAACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                    <  2:75501/149‑1 (MQ=11)
                                   aGACATGTGGAGCCGGAATGCACGCCGGCGCGAAAAGCTCATCTTTATGACCGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                  <  2:89930/149‑1 (MQ=17)
                                   aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                  >  2:297954/1‑149 (MQ=255)
                                   aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                  <  1:584273/149‑1 (MQ=255)
                                       aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGGAGATGTCGATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                              >  1:336069/1‑149 (MQ=255)
                                       aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAc                                                                                                              >  2:36106/1‑149 (MQ=255)
                                       aGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACCGCGTGGCGC‑‑GGGGAGGCTGATGCAAGGGGCCACCACGTTAACGCGGGGAAc                                                                                                              >  2:574929/1‑149 (MQ=11)
                                         gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCATCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                            >  1:453216/1‑149 (MQ=255)
                                         gTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTACACAAGCCGCTACCGGGGAGCGGGCGGAGCGGGTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                            >  1:163711/1‑149 (MQ=0)
                                          tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCCATACGGCGAGGCGC‑‑GGGGAGTATGATGGCTCAGGCACCCAGCTTAAGGAGGAGACCCCg                                                                                                           >  2:111490/1‑149 (MQ=11)
                                          tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCg                                                                                                           <  1:490999/149‑1 (MQ=255)
                                              gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAAt                                                                                                                                        <  2:648700/116‑1 (MQ=11)
                                              gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAAt                                                                                                                                        >  1:648700/1‑116 (MQ=11)
                                                  gAATCCACACCGGCGCGACAAGCCCAGCTTCATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                   <  1:301881/149‑1 (MQ=17)
                                                   aaTACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACTCtcatcacctg                                                                                                  <  2:430523/149‑5 (MQ=1)
                                                   aaTACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGC‑‑GGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctg                                                                                                  <  2:548908/149‑5 (MQ=11)
                                                        acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATggg                                                                                                                                     >  2:89883/1‑109 (MQ=255)
                                                        acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATggg                                                                                                                                     <  1:89883/109‑1 (MQ=255)
                                                        acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                             >  1:400575/1‑149 (MQ=255)
                                                        acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                             >  1:118937/1‑149 (MQ=255)
                                                               cgcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAc                                                                                      <  2:302987/149‑1 (MQ=255)
                                                                gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCGCCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                     <  1:539049/149‑1 (MQ=255)
                                                                    aatgCTCATCTCTATCCCTGTGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                                 <  1:204652/146‑1 (MQ=255)
                                                                        cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCa                                                                             <  2:268167/149‑1 (MQ=255)
                                                                         tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                            >  1:546242/1‑149 (MQ=255)
                                                                         tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                            >  1:546470/1‑149 (MQ=255)
                                                                                tATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                                                     <  1:610197/149‑1 (MQ=255)
                                                                                     cTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatg                                                                >  2:679253/1‑149 (MQ=255)
                                                                                     cTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatg                                                                >  1:395085/1‑149 (MQ=255)
                                                                                            ccaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                       >  1:349722/1‑88 (MQ=255)
                                                                                            ccaccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                       <  2:349722/88‑1 (MQ=255)
                                                                                               ccGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAAt                                                       >  2:455012/1‑149 (MQ=255)
                                                                                                cGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATc                                                      >  1:518752/1‑149 (MQ=255)
                                                                                                    gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTGTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                  <  2:64402/149‑1 (MQ=255)
                                                                                                          gcGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGGGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                             <  1:554630/148‑1 (MQ=255)
                                                                                                          ccGGTGGGGCTTATCAGCGAAGGCGGCAATACGGGGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                             <  2:125189/149‑1 (MQ=255)
                                                                                                          ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGAGCGGAAGAGTGTAGTGTGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCATCAGCATAATGAGGAATGAGGGCTGTa                                             >  2:634376/1‑149 (MQ=17)
                                                                                                          ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGGACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATACGGGCTGtg                                             >  1:328591/1‑148 (MQ=255)
                                                                                                          ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                             <  2:400575/149‑1 (MQ=255)
                                                                                                                ccgcTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                                       <  2:61898/148‑1 (MQ=255)
                                                                                                                 cgcTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAc                                      <  1:36106/149‑1 (MQ=255)
                                                                                                                     tATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATAAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATTACCGtt                                  >  2:62654/1‑148 (MQ=255)
                                                                                                                       tCAACGAAGTCGGCAATCAGGCGTGGAGC‑‑GCTGAACATTATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCAc                                <  1:238657/149‑1 (MQ=17)
                                                                                                                            gAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGta                           >  2:436933/1‑149 (MQ=255)
                                                                                                                                aCGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAACCGCATTATGTGTAGCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGCGTGTACTATAACCTTCACCGGgactac                       >  1:321779/1‑149 (MQ=17)
                                                                                                                                  ggCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                            >  1:8962/1‑110 (MQ=255)
                                                                                                                                  ggCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                            <  2:8962/110‑1 (MQ=255)
                                                                                                                                       caCGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGt                <  2:204579/148‑1 (MQ=255)
                                                                                                                                                       cgGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGtat  >  1:35447/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                    
TTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGC‑‑GCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTAT  >  NC_000913_3_pae_pgi/526649‑526944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: