New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi 2505261 =31 (1.320)4 (0.180) 3/274 3.1 12.4% coding (287/1038 nt) ypdE aminopeptidase
?NC_000913_3_pae_pgi 2505282 = 27 (1.200)coding (266/1038 nt) ypdE aminopeptidase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCA  >  NC_000913_3_pae_pgi/2505137‑2505428
                                                                                                                                                 |                                                                                                                                                  
cTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGc                                                                                                                                                 <  2:73573/149‑1 (MQ=255)
cTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGc                                                                                                                                                 <  1:333083/149‑1 (MQ=255)
        cTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTg                                                                                                                                         >  2:257740/1‑149 (MQ=255)
            gTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCCGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCaa                                                                                                                                     <  1:333967/149‑1 (MQ=255)
              cATAGGAGCGCGCACCAATGTCCCCGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCCGCTGGCGGGCAGCCATACGTACGTTGCCAAcc                                                                                                                                   >  2:119266/1‑149 (MQ=255)
                        cgcACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACAt                                                                                                                         <  1:257740/149‑1 (MQ=255)
                          cACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGGGGGAAGCCATGCGTACGTTGCCAGCCGGCGGACCATCa                                                                                                                       >  1:227226/1‑149 (MQ=255)
                                tGTCCACGCGCATGGCGCTGACGTCATTCCCCGGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGcc                                                                                                                 <  2:276843/149‑1 (MQ=255)
                                         gcATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCgg                                                                                                        <  2:353468/149‑1 (MQ=255)
                                         gcAGGGCGCTGACGGCCTTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACTTGGTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCgg                                                                                                        <  1:97304/149‑1 (MQ=255)
                                            tGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACACCAATCGCCCCTTCGCGGgag                                                                                                     >  1:339170/1‑149 (MQ=255)
                                                      gTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCa                                                                                           >  1:241932/1‑149 (MQ=255)
                                                              gcTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCCGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAAt                                                                                   <  2:113617/148‑1 (MQ=255)
                                                                  ccGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCa                                                                               <  2:142908/149‑1 (MQ=255)
                                                                          cGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTcc                                                                       <  1:299223/149‑1 (MQ=255)
                                                                                gCAGGCCTGGAATTTTGCACTCGTCACGGGTGTTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATgc                                                                 <  2:243194/149‑1 (MQ=255)
                                                                                 cAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATgcg                                                                >  1:263684/1‑149 (MQ=255)
                                                                                        ggAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACCTCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGat                                                         <  1:368799/149‑1 (MQ=255)
                                                                                            ttttGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGatcatc                                                     <  1:192580/149‑1 (MQ=255)
                                                                                                 cACTCTGCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCATCATAAAGCCCACTTCGTCCATATGCGCACAGATCATCACCtt                                                <  1:223676/149‑1 (MQ=255)
                                                                                                                   aTGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGattcatt                              >  2:319410/1‑149 (MQ=255)
                                                                                                                        cACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGc                         >  1:240293/1‑149 (MQ=255)
                                                                                                                           cGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGa                      <  1:377241/149‑1 (MQ=255)
                                                                                                                            ggCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAGTCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGAt                     <  1:285131/149‑1 (MQ=255)
                                                                                                                                gCAGCTGGGGGGCGGCCAGGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGc                 <  2:35766/149‑1 (MQ=255)
                                                                                                                                  cgCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGCGATATGCTTCGCACCATAAATCCCACTTCGTCCATATTCGCACAGATCATCACCTTCTGACCTGTCGATTCTTTCAGGCGGACCCGCAc               >  2:142374/2‑149 (MQ=255)
                                                                                                                                   gCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCAcc              >  1:385238/1‑149 (MQ=255)
                                                                                                                                               ccATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCa  >  1:356601/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTGCATCACTTCGTCATAGGAGCGCGCACCAATGTCCACGCGCATGGCGCTGACGTCATTCCCCTGCCGGTCGCCGTCAAGCAGGCCTGGAATTTTGCACTCTTCACGGGTGGTGATGCGCACCGGCTGCAGCTGGCGGGCAGCCATGCGTACGTTGCCAACCGGCAGCACATCAATCGCCCCTTCGCGGGAGATGCTGCGCACCATAAATCCCACTTCGTCCATATGCGCACAGATCATCACCTTCGGACCTGTCGATTCATTCAGGCGGATCAGCACCGATCCCAGACCA  >  NC_000913_3_pae_pgi/2505137‑2505428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.