Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,234,667 Δ1 bp intergenic (‑1434/‑499) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,234,6660A.98.6% 303.2 / ‑1.2 70intergenic (‑1433/‑500)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (30/38);  total (31/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.76e-01

TATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAC  >  NC_000913_3_pae_pgi/4234522‑4234798
                                                                                                                                                |                                                                                                                                    
tatatgGGCAGTAGAGACGAGCCCCAAGGGGGGTTTATCCGTTTTACGAGAGGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCTAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatcatc                                                                                                                                <  1:217756/150‑1 (MQ=255)
tatataCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatcatc                                                                                                                                >  1:102991/1‑150 (MQ=255)
 atataCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCGGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcg                                                                                                                               >  2:678465/1‑150 (MQ=255)
   ttaCGGAGTAGAGATGCCCCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgtc                                                                                                                             <  2:579909/149‑1 (MQ=255)
    taCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCACTGAAGAGGCGCTCGCAGTGATCAtcgtct                                                                                                                            >  2:590385/1‑149 (MQ=255)
     aCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGa                                                                                                                           <  2:892260/150‑1 (MQ=255)
     aCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGa                                                                                                                           <  1:243596/150‑1 (MQ=255)
      cGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGCGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGat                                                                                                                          <  1:325955/150‑1 (MQ=255)
       gCAGTAGAGATGCACCCCAAGGGCGTTTTATAATTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGCATAGATGCATTGAAGAGACGCTTGCCGTGATCATCATCAata                                                                                                                         >  2:213742/1‑150 (MQ=255)
               gaTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTTTAAAACTGGCAGAGATTAGGGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCATTGATCACGGTTGGTATGTATGcc                                                                                                                 >  2:468552/1‑150 (MQ=255)
                 tGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTACAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCgg                                                                                                               >  1:651279/1‑150 (MQ=255)
                  gCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCTTCGATATGTAGGCCGGa                                                                                                              >  2:794682/1‑150 (MQ=255)
                    aCCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTGGATACTGAGACCGGATa                                                                                                            >  2:598294/1‑150 (MQ=255)
                        cAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                        <  1:598294/150‑1 (MQ=255)
                        cAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                        <  2:517219/150‑1 (MQ=255)
                             gCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCa                                                                                                   <  1:371490/150‑1 (MQ=255)
                             gCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCa                                                                                                   >  1:430942/1‑150 (MQ=255)
                                ttttATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCCcgc                                                                                                >  2:190588/1‑150 (MQ=255)
                                 tttATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgcc                                                                                               <  2:350427/150‑1 (MQ=255)
                                  ttATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgt                                                                                                                              >  1:138122/1‑118 (MQ=255)
                                  ttATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgt                                                                                                                              <  2:138122/118‑1 (MQ=255)
                                    aTCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGCTAAGGCGTTCACGCCGCa                                                                                            >  1:408405/1‑150 (MQ=255)
                                       cTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGAAGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATcc                                                                                         <  1:623245/150‑1 (MQ=255)
                                        tttttACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGCTGCATTGACGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCg                                                                                        >  1:135715/1‑150 (MQ=255)
                                            tACGAGATGTGGGCATCGCCTTGGTAACCGTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                    <  2:408405/150‑1 (MQ=39)
                                               gagaTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCg                                                                                 >  2:532451/1‑150 (MQ=255)
                                                 gaTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                               <  1:617740/150‑1 (MQ=255)
                                                  aTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATg                                                                              >  1:279736/1‑150 (MQ=255)
                                                   tgtgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGc                                                                             <  2:403219/150‑1 (MQ=255)
                                                    gtgGGCATCGCCTCTGTAAAACTGGCAGAGATTCGATATTTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                                                            >  1:972213/1‑150 (MQ=39)
                                                          aTCGCCTTTGTAGAACTGGCAGAGATTAGAGGCTTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGc                                                                      <  2:453656/150‑1 (MQ=255)
                                                          aTCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGGGCTCATCGTCGATATGTCGGCCGGATAAGGCGTTCACGCCACATCCGGCAACTTATGCCtttaac                                                                      >  2:321454/1‑145 (MQ=255)
                                                             gCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGGGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGGGTTCCAGCCGCATCCGGCAACCGATGCCGGAGTCGAc                                                                   >  2:449730/1‑150 (MQ=255)
                                                               ctctGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGat                                                                                                                                     >  2:426201/1‑82 (MQ=255)
                                                               ctctGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGat                                                                                                                                     <  1:426201/83‑2 (MQ=255)
                                                                  tGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCCCGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATACGGCAACCGATGCCCGCTGGGACGCGGt                                                              >  2:129735/1‑150 (MQ=38)
                                                                     aaaaCTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTcgc                                                           <  2:828490/150‑1 (MQ=255)
                                                                         cTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTTATGCGACGCGGTCGCGTCt                                                       >  2:522059/1‑150 (MQ=255)
                                                                          tGGCAGAGATTAGAGGCGTAAATCCAATGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCtt                                                      >  2:709358/1‑150 (MQ=255)
                                                                           ggtaaggaTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTa                                                     <  2:100575/144‑1 (MQ=255)
                                                                            gCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTAt                                                    >  1:729999/1‑150 (MQ=255)
                                                                              agagATTGGAGGCGTAAATCCCCTGGCCATCCCGGGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                  <  2:556387/150‑1 (MQ=255)
                                                                              agaaATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATGGAAGAAGCACTTGCAGCGATCATCGTCGCCATGTAGGCCGGATGACGGGTTCAAGCCGCCTCCGGCACCCTATGCCTGATTCGACACTGTACCGTCatatac                                                  >  2:474619/1‑148 (MQ=255)
                                                                               gagaTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAg                                                 <  1:749683/150‑1 (MQ=255)
                                                                                  aTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                              <  1:678465/150‑1 (MQ=255)
                                                                                       agGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                         <  2:651279/150‑1 (MQ=255)
                                                                                       agGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAg                                         <  2:102991/150‑1 (MQ=255)
                                                                                         gCGTAAATCCCCTGGCCACCCCAAGAATAGATGCATTGAAGCGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                       <  1:546704/150‑1 (MQ=255)
                                                                                            tAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa                                    <  2:727463/150‑1 (MQ=255)
                                                                                            tAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa                                    <  1:129735/150‑1 (MQ=255)
                                                                                               aTCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGat                                                                                                                                     <  2:227871/51‑2 (MQ=255)
                                                                                               aTCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGat                                                                                                                                     >  1:227871/1‑50 (MQ=255)
                                                                                                  cccTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGa                              <  1:419184/150‑1 (MQ=255)
                                                                                                     tGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCCGCAACCGATGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                           <  2:859764/150‑1 (MQ=255)
                                                                                                        ccACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTa                        <  1:537232/150‑1 (MQ=255)
                                                                                                          aCCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                      <  1:709358/150‑1 (MQ=255)
                                                                                                          aCCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCAGCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACa                      <  1:138975/150‑1 (MQ=255)
                                                                                                            cccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                    >  2:262409/1‑150 (MQ=255)
                                                                                                            cccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                    >  1:567131/1‑150 (MQ=255)
                                                                                                             ccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCGCATATGtcctact                   >  2:560770/1‑144 (MQ=25)
                                                                                                             ccGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                   >  1:218579/1‑150 (MQ=255)
                                                                                                                 gaATATGTGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTAtt               <  2:99789/150‑1 (MQ=255)
                                                                                                                  aaTAGATGCAGTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc              <  2:648984/150‑1 (MQ=255)
                                                                                                                   aTAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCg             <  1:190588/150‑1 (MQ=255)
                                                                                                                       aTGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                    >  2:342461/1‑139 (MQ=255)
                                                                                                                       aTGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATc                    <  1:342461/139‑1 (MQ=255)
                                                                                                                       aTGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCaa         >  1:323317/1‑150 (MQ=255)
                                                                                                                         gCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                           >  1:141382/1‑130 (MQ=255)
                                                                                                                         gCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGatat                           <  2:141382/130‑1 (MQ=255)
                                                                                                                           aTTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCTGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaa     >  2:563334/1‑150 (MQ=255)
                                                                                                                           aTTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaa     <  1:295769/150‑1 (MQ=255)
                                                                                                                             tGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATa   <  1:532451/150‑1 (MQ=255)
                                                                                                                              gAAGGGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGTCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc  <  1:872717/150‑1 (MQ=255)
                                                                                                                              gAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc  <  2:972213/150‑1 (MQ=255)
                                                                                                                               aaGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg                                                                >  1:373106/1‑87 (MQ=255)
                                                                                                                               aaGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAcgcg                                                                <  2:373106/87‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                    
TATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAC  >  NC_000913_3_pae_pgi/4234522‑4234798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: