Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi526,7920AC26.4% 87.2 / 34.2 53S1178R (AGC→CGC) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (16/23);  new base C (6/8);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGC  >  NC_000913_3_pae_pgi/526648‑526939
                                                                                                                                                 |                                                                                                                                                   
cTTGCGCAGTGGGGGCTGCCGGTGGAGCAACTGGCCAGACAGGGGGAGCCGGAATACCCACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGcgcgg                                                                                                                                                 <  1:115705/149‑2 (MQ=14)
 ttGCGCAGTGCGGGCTGACGGGTGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGcgcg                                                                                                                                                <  1:420023/149‑1 (MQ=255)
 ttGCGCAGGGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGcgcggg                                                                                                                                                <  1:416991/149‑3 (MQ=255)
         tGCGGGCGGACGGTGGAGCAACTGGCCAGACACGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                        <  1:45525/149‑1 (MQ=255)
             ggCTGACGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGaa                                                                                                                                    <  2:118888/149‑1 (MQ=11)
               cTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                       <  1:293207/144‑1 (MQ=255)
               cTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                       >  2:293207/1‑144 (MQ=255)
                tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                 <  1:415905/149‑1 (MQ=255)
                    ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatg                                                                                                                                         <  1:339098/137‑1 (MQ=255)
                    ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatg                                                                                                                                         >  2:339098/1‑137 (MQ=255)
                      tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                           <  2:430098/149‑1 (MQ=255)
                             aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCGGCGTa                                                                                                                    >  2:485224/1‑149 (MQ=255)
                               tGGCCAGGCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                  <  2:453663/149‑1 (MQ=11)
                                 gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTGATCTTTATCACTGCGACCACCGGGGACTGCCGATGGCGCTGAT‑AGTGTAAGACGGGAATACGGCGGGGAGCGCGGAGTATGAAGACCGTGGCCAGCAGAATAATga                                                                                                                >  2:504453/1‑149 (MQ=14)
                                 gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                <  2:57557/149‑1 (MQ=255)
                                   cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                              >  1:495632/1‑149 (MQ=255)
                                   cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                              <  2:495632/149‑1 (MQ=255)
                                   cAGACAGGTGGAATAGAAATACACACCGGCGCGAACAGCTCATCTTTACCACCTAGAGCACCTTTGACGGCAGCTCGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                              <  1:251069/149‑1 (MQ=14)
                                    aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                             <  1:487098/149‑1 (MQ=255)
                                     gACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGGATGATGAATGGGGCAACCAGCTTAAGgaggag                                                                                                            >  2:38965/1‑149 (MQ=11)
                                      aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGcgcg                                                                                                                                                  <  2:25418/110‑1 (MQ=255)
                                      aCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGcgcg                                                                                                                                                  >  1:25418/1‑110 (MQ=255)
                                           tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                               <  1:49715/124‑1 (MQ=255)
                                           tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                               >  2:49715/1‑124 (MQ=255)
                                             gAGCCGGAAGACACACCGGCGCGAAAAGCTCATCTTGATCACTGGCACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                                    <  2:261269/149‑1 (MQ=255)
                                               gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                  >  1:27465/1‑149 (MQ=255)
                                               gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAAGAGAACACTAGTTTGTTGAGGAGCACGCGcatc                                                                                                  >  1:248554/1‑149 (MQ=17)
                                                   gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                              <  2:347523/149‑4 (MQ=11)
                                                   gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                              <  1:3347/149‑1 (MQ=255)
                                                      tACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgca                                                                                           >  2:43146/1‑143 (MQ=11)
                                                        cacacCGGCGCGAAAAGCCCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                         <  1:3140/149‑1 (MQ=255)
                                                                 gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                       <  1:469137/110‑1 (MQ=11)
                                                                 gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                       >  2:469137/1‑110 (MQ=14)
                                                                     aaaGCTCATCTTTATCACTGCGACCACCGGGTACTGCCGCTGGGGTTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                            <  1:378337/149‑1 (MQ=255)
                                                                          tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                              >  2:129305/1‑123 (MQ=1)
                                                                          tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                              <  1:129305/126‑4 (MQ=1)
                                                                            aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                               <  2:66835/107‑1 (MQ=255)
                                                                            aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                               >  1:66835/1‑107 (MQ=255)
                                                                               tttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCCATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACACGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcag                                                                  >  1:259240/1‑149 (MQ=255)
                                                                                 tATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                                                <  2:119417/149‑1 (MQ=255)
                                                                                 tATCACTGCGACCACCGGGGACGGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCCTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                                                <  1:100851/149‑1 (MQ=255)
                                                                                        gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGTAGCatgat                                                         <  1:471465/149‑1 (MQ=255)
                                                                                           accaccGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCCGCGTAATTAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAg                                                      <  1:485224/149‑1 (MQ=255)
                                                                                             caccGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGa                                                    >  1:502678/1‑149 (MQ=255)
                                                                                                  gggACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAg                                               <  1:333690/149‑1 (MQ=255)
                                                                                                   ggACTGCCGCTGGCGCTTATCAGCG‑AAGCCGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATTATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAgg                                              <  2:263297/149‑1 (MQ=255)
                                                                                                       tGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                          <  2:502678/149‑1 (MQ=255)
                                                                                                         ccGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAGTGAGGAGCACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGCTGAGGAATCAGGGCTGTa                                        >  2:144270/1‑149 (MQ=255)
                                                                                                          cGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                >  1:308483/1‑108 (MQ=255)
                                                                                                          cGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                <  2:308483/108‑1 (MQ=255)
                                                                                                               gcgtTTATCAGCG‑AAGATGGCAATACGGCGTGGAGCGCTGAATATGATGAATGGCGCAACCAGCTTAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                                  <  1:288879/149‑1 (MQ=17)
                                                                                                                 gcTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAAGGCTGTACTATAAcc                                >  1:66890/1‑149 (MQ=255)
                                                                                                                         gCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCgg                        >  1:75558/1‑149 (MQ=255)
                                                                                                                                     aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                         >  1:74983/1‑105 (MQ=255)
                                                                                                                                     aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                         <  2:74983/105‑1 (MQ=255)
                                                                                                                                       tACGGCGTGGAGGGCGGAATATGATGGATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCGGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTGTAACCGTCACCGGTAGTACGATCCGt           >  2:141213/1‑149 (MQ=17)
                                                                                                                                                gAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGc  >  2:173132/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CTTGCGCAGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGC  >  NC_000913_3_pae_pgi/526648‑526939

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑