Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi526,8000AG28.3% 94.3 / 31.6 53E1180E (GAA→GAG) rhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (15/23);  new base G (7/8);  total (22/31)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.55e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGT  >  NC_000913_3_pae_pgi/526657‑526942
                                                                                                                                                |                                                                                                                                              
tGCGGGCGGACGGTGGAGCAACTGGCCAGACACGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                           <  1:45525/149‑1 (MQ=255)
    ggCTGACGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGaa                                                                                                                                       <  2:118888/149‑1 (MQ=11)
      cTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                          >  2:293207/1‑144 (MQ=255)
      cTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatgat                                                                                                                                          <  1:293207/144‑1 (MQ=255)
       tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                    <  1:415905/149‑1 (MQ=255)
           ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatg                                                                                                                                            >  2:339098/1‑137 (MQ=255)
           ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATatg                                                                                                                                            <  1:339098/137‑1 (MQ=255)
             tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                              <  2:430098/149‑1 (MQ=255)
                    aCTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCGGCGTa                                                                                                                       >  2:485224/1‑149 (MQ=255)
                      tGGCCAGGCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                     <  2:453663/149‑1 (MQ=11)
                        gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTGATCTTTATCACTGCGACCACCGGGGACTGCCGATGGCGCTGAT‑AGTGTAAGACGGGAATACGGCGGGGAGCGCGGAGTATGAAGACCGTGGCCAGCAGAATAATga                                                                                                                   >  2:504453/1‑149 (MQ=14)
                        gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                   <  2:57557/149‑1 (MQ=255)
                          cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                 <  2:495632/149‑1 (MQ=255)
                          cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                 >  1:495632/1‑149 (MQ=255)
                          cAGACAGGTGGAATAGAAATACACACCGGCGCGAACAGCTCATCTTTACCACCTAGAGCACCTTTGACGGCAGCTCGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                 <  1:251069/149‑1 (MQ=14)
                           aGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagga                                                                                                                <  1:487098/149‑1 (MQ=255)
                            gACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGGATGATGAATGGGGCAACCAGCTTAAGgaggag                                                                                                               >  2:38965/1‑149 (MQ=11)
                                  tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                                  >  2:49715/1‑124 (MQ=255)
                                  tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATgggg                                                                                                                                  <  1:49715/124‑1 (MQ=255)
                                    gAGCCGGAAGACACACCGGCGCGAAAAGCTCATCTTGATCACTGGCACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGca                                                                                                       <  2:261269/149‑1 (MQ=255)
                                      gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                     >  1:27465/1‑149 (MQ=255)
                                      gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAAGAGAACACTAGTTTGTTGAGGAGCACGCGcatc                                                                                                     >  1:248554/1‑149 (MQ=17)
                                          gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                                 <  2:347523/149‑4 (MQ=11)
                                          gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                 <  1:3347/149‑1 (MQ=255)
                                             tACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgca                                                                                              >  2:43146/1‑143 (MQ=11)
                                               cacacCGGCGCGAAAAGCCCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATc                                                                                            <  1:3140/149‑1 (MQ=255)
                                                        gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                          <  1:469137/110‑1 (MQ=11)
                                                        gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGc                                                                                                                          >  2:469137/1‑110 (MQ=14)
                                                            aaaGCTCATCTTTATCACTGCGACCACCGGGTACTGCCGCTGGGGTTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                               <  1:378337/149‑1 (MQ=255)
                                                                 tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                                 <  1:129305/126‑4 (MQ=1)
                                                                 tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                                 >  2:129305/1‑123 (MQ=1)
                                                                   aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                  >  1:66835/1‑107 (MQ=255)
                                                                   aTCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgag                                                                                                                  <  2:66835/107‑1 (MQ=255)
                                                                      tttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCCATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACACGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcag                                                                     >  1:259240/1‑149 (MQ=255)
                                                                        tATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                                                   <  2:119417/149‑1 (MQ=255)
                                                                        tATCACTGCGACCACCGGGGACGGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCCTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagca                                                                   <  1:100851/149‑1 (MQ=255)
                                                                               gCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGTAGCatgat                                                            <  1:471465/149‑1 (MQ=255)
                                                                                  accaccGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCCGCGTAATTAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAg                                                         <  1:485224/149‑1 (MQ=255)
                                                                                    caccGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGa                                                       >  1:502678/1‑149 (MQ=255)
                                                                                         gggACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAg                                                  <  1:333690/149‑1 (MQ=255)
                                                                                          ggACTGCCGCTGGCGCTTATCAGCG‑AAGCCGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATTATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAgg                                                 <  2:263297/149‑1 (MQ=255)
                                                                                              tGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                             <  2:502678/149‑1 (MQ=255)
                                                                                                ccGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAGTGAGGAGCACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGCTGAGGAATCAGGGCTGTa                                           >  2:144270/1‑149 (MQ=255)
                                                                                                 cGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                   <  2:308483/108‑1 (MQ=255)
                                                                                                 cGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                   >  1:308483/1‑108 (MQ=255)
                                                                                                      gcgtTTATCAGCG‑AAGATGGCAATACGGCGTGGAGCGCTGAATATGATGAATGGCGCAACCAGCTTAATGAGGAGACCCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATaa                                     <  1:288879/149‑1 (MQ=17)
                                                                                                        gcTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAAGGCTGTACTATAAcc                                   >  1:66890/1‑149 (MQ=255)
                                                                                                                gCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCgg                           >  1:75558/1‑149 (MQ=255)
                                                                                                                            aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                            <  2:74983/105‑1 (MQ=255)
                                                                                                                            aaTACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCatgat                                                            >  1:74983/1‑105 (MQ=255)
                                                                                                                              tACGGCGTGGAGGGCGGAATATGATGGATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCGGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTGTAACCGTCACCGGTAGTACGATCCGt              >  2:141213/1‑149 (MQ=17)
                                                                                                                                       gAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGc     >  2:173132/1‑149 (MQ=255)
                                                                                                                                          cgcgGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGt  <  1:426968/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCG‑AAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGT  >  NC_000913_3_pae_pgi/526657‑526942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑