| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913_3_pae_pgi | 4,295,641 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913_3_pae_pgi | 4,295,640 | 1 | . | C | 90.0% | 76.1 / 3.0 | 30 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base C (15/12); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913_3_pae_pgi | 4,295,640 | 2 | . | G | 90.0% | 73.2 / 2.7 | 30 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
| Reads supporting (aligned to +/- strand): ref base . (0/3); new base G (15/12); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCC > NC_000913_3_pae_pgi/4295557‑4295777 || aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACAGt > 1:392719/1‑149 (MQ=2) cgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt < 2:615179/149‑1 (MQ=255) cgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAttt > 1:284819/1‑149 (MQ=255) gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATACGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGGTAAtttt > 2:962599/1‑149 (MQ=14) tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAGTTTTTCaa > 1:547454/1‑149 (MQ=255) tCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCCACGCTTGCCGCCTCTTATCCGGCCTATCTTCACCGTTGTTTAATTTTTCacac > 2:243855/1‑149 (MQ=11) ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAActct > 2:1058226/1‑149 (MQ=255) cTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGGCGCGTCGCATCAGGCCTCTCTTACCCGCTGGTTAATTTTCCAACCTCTGca > 1:419326/1‑149 (MQ=11) ccAGACAGCGGAATAACCTCATTTAGCGTGATTTTATAGGTCGGATAAGGCGGTT‑‑ACGC‑‑CG‑CATCCGACATCAATTCCTCATGCGACGCTTTCCGCGGCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc < 2:21008/149‑1 (MQ=17) aGACATCGCGATAGCCTGATTTATCGTGATTTTGTAGATCGGATAAGTCGTTT‑‑A‑‑CTGCG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCgg < 2:1033710/149‑1 (MQ=18) aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCgg < 2:558767/149‑1 (MQ=255) gcAATAGCCTTATGTAGCGTTATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:256401/149‑1 (MQ=18) gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACAGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCaaa < 2:392719/149‑1 (MQ=255) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt > 1:541598/1‑139 (MQ=255) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGt < 2:541598/139‑1 (MQ=255) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCa < 1:363140/149‑1 (MQ=255) aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc < 2:253097/149‑1 (MQ=255) aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTAACACCCGGTATCAAACCCTTCCATAc > 1:253097/1‑149 (MQ=255) tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa > 1:189104/1‑147 (MQ=255) tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATa < 2:189104/147‑1 (MQ=255) cGTGATTTTGTAGGTCGGGTAAGGCCTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa < 1:728723/149‑1 (MQ=25) cGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa > 1:895284/1‑149 (MQ=255) gATTTTGTAGGTGGGATAAGGCGTTT‑‑A‑‑CG‑CGCCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGt < 2:640836/149‑1 (MQ=25) gATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCACCACCGGTCTCAAACCCTTCCTTCCAGCTCCGGt > 1:76381/1‑149 (MQ=18) ttGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCGTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCTAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCaa > 2:62365/1‑149 (MQ=25) ggTCGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCATACCCTTGCATACAGTTTAGGTTCAACCAGt > 1:416800/1‑149 (MQ=25) cGGATAAGGCGTTTCCG‑‑C‑‑CG‑CATGCGACATCCATGCCTGTTTCGGCGCTGGCAGTGGCCTATCACGCCTATCTTATGCGTGGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa < 1:747387/149‑1 (MQ=255) cGGATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCTTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCa < 1:746430/149‑1 (MQ=255) ggATAAGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTCCTGTTTCTGAGCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAg < 1:206181/149‑1 (MQ=37) aaGGCGTTT‑‑ACGC‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGcc > 1:592100/1‑149 (MQ=255) || ACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑CG‑CATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCC > NC_000913_3_pae_pgi/4295557‑4295777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |