New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_pae_pgi = 123568542 (0.760)5 (0.100) 5/264 7.0 11.2% intergenic (+28/+24) fadR/ycgB fatty acid metabolism regulon transcriptional regulator/SpoVR family stationary phase protein
?NC_000913_3_pae_pgi = 1235689 40 (0.780)intergenic (+32/+20) fadR/ycgB fatty acid metabolism regulon transcriptional regulator/SpoVR family stationary phase protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/1235534‑1235685
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaacccctcGTTTGAGGGGTTTGCTCTTTAAACGGAAGGGATTATCGCCCCTGAATGGCTAAATCACCCGGCAGATTTTTCTGCATCCGGTGCCAAATCTCGCCACTCTCATGCCCATAGCGACGCACTGTTTCGTACACCTGATCGTGCGCGCCTT  <  NC_000913_3_pae_pgi/1235689‑1235542
                                                                                                                                                                                                                                                                                                            
ggGCAGTGAAGGCGCGCACGAGCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCC                                                                                                                                                         <  1:926797/147‑1
  GCAGTGAAGGCGCGCACGATCAGGTGGACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCT                                                                                                                                                       <  2:477856/149‑1
     GTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCGT                                                                                                                                                    >  1:358326/1‑149
                     TCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCGTTTGAGGGGTTTGCTCT                                                                                                                                    <  2:358326/149‑1
                                 AACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCGTTTGAGGGGTTTGCTCTTTAAACGGAAGG                                                                                                                        <  1:275646/149‑1
                                                                   ATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCGTTTGAGGGGTTTGCTCTTTAAACGGAAGGGATTATCGCCC                                                                                                             <  1:177980/126‑1
                                                                   ATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCGTTTGAGGGGTTTGCTCTTTAAACGGAAGGGATTATCGCCC                                                                                                             >  2:177980/1‑126
                                                                                                                                                     CTCGTTTGAGGGGTTTGCTCTTTAAACGGAAGGGATTATCGCGCCTGAATGGCTACATCACCCGGCAGATGTTTCTGCATCCGGTGCCAAATCTCGCCACTCTCATGCCCATAGCGACGCACTGTTTCGTACACCTGATCGTGCGCGCC    >  2:453273/1‑149
                                                                                                                                                       CGTTTGAGGGGTTTGCTCTTTAAACGGAAGGGATTATCGCCCCTGAATGGCTAAATCACCCGGCAGATTTTTCTGCATCCGGTGCCAAATCTCGCCACTCTCATGCCCATAGCGACGCACTGTTTCGTACACCTGATCGTGCGCGCCTT  <  1:647383/149‑1
                                                                                                                                                                                                                                                                                                            
GTGCAGTGAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913_3_pae_pgi/1235534‑1235685
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑aaacccctcGTTTGAGGGGTTTGCTCTTTAAACGGAAGGGATTATCGCCCCTGAATGGCTAAATCACCCGGCAGATTTTTCTGCATCCGGTGCCAAATCTCGCCACTCTCATGCCCATAGCGACGCACTGTTTCGTACACCTGATCGTGCGCGCCTT  <  NC_000913_3_pae_pgi/1235689‑1235542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.