Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	2,534,871	T→C	V269A (GTA→GCA)	ptsI →	PEP‑protein phosphotransferase of PTS system (enzyme I)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	2,534,871	0	T	C	100.0%	185.9 / NA	55	V269A (GTA→GCA)	ptsI	PEP‑protein phosphotransferase of PTS system (enzyme I)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/27);  total (28/27)

GATGCCGTAAATAATCAGGTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGTAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTA  >  NC_000913_3_pae_pgi/2534726‑2535015                                                                                                                                                  |                                                                                                                                                 gATGCCGTAAATAATCAGGTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGa                                                                                                                                               >  1:505466/1‑149 (MQ=255)  aTGCCGTAAATAATCAGGTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCAGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGaa                                                                                                                                              <  1:649978/149‑1 (MQ=255)     ctgTAAATAATCAGGTTTACGTCAATACAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTa                                                                                                                                           <  1:309841/147‑1 (MQ=255)      cGTAAATAATCAGGTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTAt                                                                                                                                          <  1:917325/149‑1 (MQ=255)      cGTAAATAATCAGGTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTAt                                                                                                                                          <  1:603640/149‑1 (MQ=255)       gTAAATAATCAGGTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATg                                                                                                                                         >  1:688567/1‑149 (MQ=255)        tAAATAATCAGGTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATgc                                                                                                                                        >  2:963155/1‑149 (MQ=255)                  ggTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTg                                                                                                                              <  2:175191/149‑1 (MQ=255)                  ggTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTg                                                                                                                              <  2:96196/149‑1 (MQ=255)                  ggTATACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGCTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTg                                                                                                                              <  1:61493/149‑1 (MQ=255)                   gTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTgg                                                                                                                             >  1:31410/1‑149 (MQ=255)                         gTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGt                                                                                                                       <  2:688567/149‑1 (MQ=255)                             aTCCAAGCAACGAAGTTATTGATAAAATGCGCGTTGTGCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGt                                                                                                                   <  2:706511/149‑1 (MQ=255)                                 aaccaacGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGGATGATCTGCCAGCTACTGCGCTGGACGGTCACCAGGCAGAAGTAAGCACGAACAGTGGTACGGTTCGTGACg                                                                                                               >  2:820703/1‑149 (MQ=255)                                   ccaacGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGtt                                                                                                             <  2:505466/149‑1 (MQ=255)                                     aacGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGa                                                                                                           >  2:252351/1‑149 (MQ=255)                                      acGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGaa                                                                                                          <  2:501762/149‑1 (MQ=255)                                       cGAAGTTAGTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAg                                                                                                         <  1:551344/149‑1 (MQ=255)                                         aatgtATTGGAAAAACGCGCGGTGTTCAGGAGCAAGTGGCTTCTGAAAAATCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTTGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGt                                                                                                       <  2:178732/145‑1 (MQ=255)                                           gTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATCGGTACGGTTCGTGACGTTGAAGGTg                                                                                                      >  2:645011/1‑148 (MQ=255)                                           gTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATCGGTACGGTTCGTGACGTTGAAGGTg                                                                                                      <  1:645011/148‑1 (MQ=255)                                            ttATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCa                                                                                                    <  1:88263/149‑1 (MQ=255)                                            ttATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCa                                                                                                    >  1:553658/1‑149 (MQ=255)                                              aTTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCaga                                                                                                  <  1:903949/149‑1 (MQ=255)                                                       aggcgcgcGGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGTTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACgg                                                                                         <  2:610830/147‑1 (MQ=255)                                                               tGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAg                                                                                 >  1:323482/1‑149 (MQ=255)                                                                 ttCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGAAACGGCGCTGAAGGc                                                                               >  1:99188/1‑149 (MQ=255)                                                                     ggAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTg                                                                           <  1:741483/149‑1 (MQ=255)                                                                                  tCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTAc                                                              >  2:969376/1‑149 (MQ=255)                                                                                  tCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTAc                                                              >  1:678963/1‑149 (MQ=255)                                                                                       aaaaGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCAGTATCGTACTGGGt                                                         >  2:30109/1‑149 (MQ=255)                                                                                         aaGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTc                                                       >  1:307864/1‑149 (MQ=255)                                                                                            cAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTg                                                    >  2:371996/1‑149 (MQ=255)                                                                                                 cTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCAt                                               <  2:553658/149‑1 (MQ=255)                                                                                                    gCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGa                                            >  1:326768/1‑149 (MQ=255)                                                                                                      tAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCCGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGGGCGTAACTGCGCTGAACGCGTTGGTCTCTATCTGACGCAGTTCCTCTTCagtcacg                                          >  2:122424/1‑143 (MQ=255)                                                                                                      tAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGAcc                                          >  1:201227/1‑149 (MQ=255)                                                                                                          cTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGt                                                                                                                   <  1:124559/72‑1 (MQ=255)                                                                                                          cTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGt                                                                                                                   >  2:124559/1‑72 (MQ=255)                                                                                                           tGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTAGTACGGTTCGTGACGTTGAAGATCCAGAGCGTAATGGCTCGGTAGGCATTGGTCTGTAGCGTACTGAGTTCCTGTTCATGGACCGCGAc                                     >  2:733945/1‑149 (MQ=255)                                                                                                             aaaGATCTGCCAGCTATTACGCTGGCCGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGCCGTTGAAGGTGAAGAGCGTACCGGCGCTGAAGGCGTTGCTCTGTATCGTACTGAGTTCCTGCTCATACACCTCGAccc                                   >  2:595911/1‑147 (MQ=255)                                                                                                                    tGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGc                              >  1:938817/1‑147 (MQ=255)                                                                                                                    tGCCAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGc                              <  2:938817/147‑1 (MQ=255)                                                                                                                       cAGCTATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTCCGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTACCGTACTGAGTTCCCGTTCATGGACCCGGCCGCCCTGCCCAca                         >  2:514703/1‑148 (MQ=255)                                                                                                                           tATTACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTgaag                     <  2:421461/149‑1 (MQ=255)                                                                                                                             ttACGCTGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATTGACCGCGACGCACTGCCCCCTgaagaa                   >  1:317965/1‑149 (MQ=255)                                                                                                                                cgcgGGACGGTTACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTgaagaagaa                <  2:76742/149‑1 (MQ=255)                                                                                                                                   tGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCAt                                               >  2:189146/1‑115 (MQ=255)                                                                                                                                   tGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCAt                                               <  1:189146/115‑1 (MQ=255)                                                                                                                                   tGGACGGTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCCAACCGAGGTCCTGTTCATGGACCGCGACGCACTGGCCACTGCAGAAGAACAg             >  1:758869/1‑149 (MQ=255)                                                                                                                                         gTCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACCGTTtgct       >  2:571789/1‑149 (MQ=255)                                                                                                                                          tCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGa                                                                                                                 <  1:96860/42‑1 (MQ=255)                                                                                                                                          tCACCAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGa                                                                                                                 >  2:96860/1‑42 (MQ=255)                                                                                                                                             ccAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCtt   <  1:969376/149‑1 (MQ=255)                                                                                                                                              cAGGCAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTa  <  1:252351/149‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                 GATGCCGTAAATAATCAGGTTTACGTCAATCCAACCAACGAAGTTATTGATAAAATGCGCGCTGTTCAGGAGCAAGTGGCTTCTGAAAAAGCAGAGCTTGCTAAACTGAAAGATCTGCCAGCTATTACGCTGGACGGTCACCAGGTAGAAGTATGCGCTAACATTGGTACGGTTCGTGACGTTGAAGGTGCAGAGCGTAACGGCGCTGAAGGCGTTGGTCTGTATCGTACTGAGTTCCTGTTCATGGACCGCGACGCACTGCCCACTGAAGAAGAACAGTTTGCTGCTTA  >  NC_000913_3_pae_pgi/2534726‑2535015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑