Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 3,486,599 C→A H160Q (CAC→CAA)  crp → cAMP‑activated global transcription factor, mediator of catabolite repression

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi3,486,5990CA97.6% 296.1 / ‑0.6 84H160Q (CAC→CAA) crpcAMP‑activated global transcription factor, mediator of catabolite repression
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (41/41);  total (42/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.11e-01

GGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCA  >  NC_000913_3_pae_pgi/3486452‑3486744
                                                                                                                                                   |                                                                                                                                                 
ggACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAacc                                                                                                                                                 <  4:721627/150‑1 (MQ=255)
     ttCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACg                                                                                                                                            >  4:498812/1‑150 (MQ=255)
         gATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTAt                                                                                                                                        >  2:283230/1‑150 (MQ=255)
           tGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGc                                                                                                                                      >  1:279690/1‑150 (MQ=255)
              gTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACGGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCaaa                                                                                                                                   >  3:645883/1‑150 (MQ=255)
              gTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCaaa                                                                                                                                   <  4:345375/150‑1 (MQ=255)
              gTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCaaa                                                                                                                                   <  1:484864/150‑1 (MQ=255)
                ttGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATc                                                                                                                                 >  1:500097/1‑150 (MQ=255)
                  gTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCaa                                                                                                                               <  1:546266/150‑1 (MQ=255)
                   tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCCGACGCTATGACTCAACCGGACGGTATGCCCATCaaa                                                                                                                              >  2:333630/1‑150 (MQ=255)
                   tCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCaaa                                                                                                                              <  1:36334/150‑1 (MQ=255)
                        acaGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTAc                                                                                                                         <  2:279690/150‑1 (MQ=255)
                                cgcgTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAgg                                                                                                                 >  1:814967/1‑150 (MQ=255)
                                      gtcTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                                                                                                           <  1:740101/150‑1 (MQ=255)
                                         tGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTc                                                                                                        >  4:482152/1‑150 (MQ=255)
                                             aGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGAt                                                                                                    <  1:320647/150‑1 (MQ=255)
                                                   ttCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCgg                                                                                              >  3:697354/1‑150 (MQ=255)
                                                     cAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                                                                            <  3:498812/150‑1 (MQ=255)
                                                      agagAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTg                                                                                           >  4:197060/1‑150 (MQ=255)
                                                       gagaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGt                                                                                          >  1:35265/1‑150 (MQ=255)
                                                        agaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAACCAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGtt                                                                                         >  3:430234/1‑150 (MQ=255)
                                                        agaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGtt                                                                                         <  3:423260/150‑1 (MQ=255)
                                                         gaAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtc                                                                                        >  1:274917/1‑150 (MQ=255)
                                                           aaGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                                      <  1:47005/150‑1 (MQ=255)
                                                               gggCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGa                                                                                  <  1:34664/150‑1 (MQ=255)
                                                               gggCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGa                                                                                  <  3:480351/150‑1 (MQ=255)
                                                                ggCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGTACGGTATGCAAATCAAAATTACCCGGAAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaa                                                                                 <  4:351646/150‑1 (MQ=255)
                                                                ggCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGaa                                                                                 >  3:22761/1‑150 (MQ=255)
                                                                   aaCCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACgg                                                                                                                                           >  4:345380/1‑89 (MQ=255)
                                                                   aaCCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACgg                                                                                                                                           <  3:345380/89‑1 (MQ=255)
                                                                             tCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCa                                                                    >  1:761420/1‑150 (MQ=255)
                                                                               cTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt                                                                                                 >  1:978417/1‑119 (MQ=255)
                                                                               cTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGt                                                                                                 <  2:978417/119‑1 (MQ=255)
                                                                                tCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTc                                                                 >  2:588448/1‑150 (MQ=255)
                                                                                tCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTc                                                                 <  1:283230/150‑1 (MQ=255)
                                                                                  gACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTg                                                               <  2:814967/150‑1 (MQ=255)
                                                                                          gggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTg                                                                           <  4:25460/130‑1 (MQ=255)
                                                                                          gggCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTg                                                                           >  3:25460/1‑130 (MQ=255)
                                                                                            gCCGCATCGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTgg                                                     <  2:8310/150‑1 (MQ=255)
                                                                                             ccGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGa                                                    >  4:682780/1‑150 (MQ=255)
                                                                                               gCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAg                                                  >  4:288154/1‑150 (MQ=255)
                                                                                                  ttGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATc                                               <  3:682780/150‑1 (MQ=255)
                                                                                                    gCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAg                                             <  4:697354/150‑1 (MQ=255)
                                                                                                     cacaGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGa                                            <  4:421762/150‑1 (MQ=255)
                                                                                                       caGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAAc                                          <  1:290618/150‑1 (MQ=255)
                                                                                                        aGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                                                                                                         <  1:882265/86‑1 (MQ=255)
                                                                                                        aGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGt                                                                                                         >  2:882265/1‑86 (MQ=255)
                                                                                                           ctctGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGa                                      >  4:638961/1‑150 (MQ=255)
                                                                                                            tctGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                                                          <  3:77098/113‑1 (MQ=255)
                                                                                                            tctGCTGAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTgg                                                                          >  4:77098/1‑113 (MQ=255)
                                                                                                              tgctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGAc                                                                       <  4:227383/114‑1 (MQ=255)
                                                                                                              tgctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGAc                                                                       >  3:227383/1‑114 (MQ=255)
                                                                                                                ctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTc                                                                                                        <  1:622170/79‑1 (MQ=255)
                                                                                                                ctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTc                                                                                                        <  3:263419/79‑1 (MQ=255)
                                                                                                                ctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTc                                                                                                        >  2:622170/1‑79 (MQ=255)
                                                                                                                ctgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTc                                                                                                        >  4:263419/1‑79 (MQ=255)
                                                                                                                 tgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAACTTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGAt                                                          >  1:1012076/1‑124 (MQ=255)
                                                                                                                 tgAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGAt                                                          <  2:1012076/124‑1 (MQ=255)
                                                                                                                  gAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCCAAATTACCCGTCAGGAAATTGGTCAGCTAGTAGGCTGTTCTCGTGACAAAGTGGGACGCATACTGAAGCTGCTGGAAGATCAGAACCTGATCTCCGc                               <  3:475872/150‑1 (MQ=255)
                                                                                                                  gAATCTGGCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGc                               >  4:296037/1‑150 (MQ=255)
                                                                                                                        ggCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                                                      <  3:227969/121‑1 (MQ=255)
                                                                                                                        ggCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTg                                                      >  4:227969/1‑121 (MQ=255)
                                                                                                                        ggCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACgg                         >  1:1035441/1‑150 (MQ=255)
                                                                                                                         gCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                                      >  3:723114/1‑88 (MQ=255)
                                                                                                                         gCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                                      <  4:723114/88‑1 (MQ=255)
                                                                                                                         gCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                                      >  2:959367/1‑88 (MQ=255)
                                                                                                                         gCAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc                                                                                      <  1:959367/88‑1 (MQ=255)
                                                                                                                          cAAAACAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTa                       >  3:444313/1‑150 (MQ=255)
                                                                                                                           aaaaCAACCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaa                      >  3:264577/1‑150 (MQ=255)
                                                                                                                             aacaacCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTaaaa                    <  3:212478/150‑1 (MQ=255)
                                                                                                                              acaacCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                                                                            <  2:825312/77‑1 (MQ=255)
                                                                                                                              acaacCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCt                                                                                            >  1:825312/1‑77 (MQ=255)
                                                                                                                                aacCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                                             <  3:477571/106‑1 (MQ=255)
                                                                                                                                aacCAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGGCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGaa                                                             >  4:477571/1‑106 (MQ=255)
                                                                                                                                   cAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                                                >  1:629435/1‑100 (MQ=255)
                                                                                                                                   cAGACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCt                                                                <  2:629435/100‑1 (MQ=255)
                                                                                                                                     gACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGAc                                                                       >  2:46324/1‑91 (MQ=255)
                                                                                                                                     gACGCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGAc                                                                       <  1:46324/91‑1 (MQ=255)
                                                                                                                                        gCTATGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTCAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCAtcgtcgct         >  1:47336/1‑148 (MQ=255)
                                                                                                                                         ctgtgtcTCAACCGGCCGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGttt        <  3:553390/144‑1 (MQ=255)
                                                                                                                                            tGACTCAACCGGAGGGTGTGCTAATCAAAATTACCCGTCAGGAAATTGGTCTTATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAATCTGAAGATGCTGGCAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACg     <  4:344006/150‑1 (MQ=255)
                                                                                                                                            tGACTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACg     <  2:274917/150‑1 (MQ=255)
                                                                                                                                              aCTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGACACCGTGGGACGCATCCTGAAGATGCTGGAAGAGCAGAACCTGATCTCCGCACACGGTAAAACCAACGTCGTTTACGGc   >  3:639099/1‑150 (MQ=255)
                                                                                                                                              aCTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGc   >  3:7523/1‑150 (MQ=255)
                                                                                                                                               cTCAACCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCa  <  4:22761/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCATTCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCA  >  NC_000913_3_pae_pgi/3486452‑3486744

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑