Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,399,449 G→T S26R (AGC→AGA fnr ← oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,399,4490GT95.0% 339.3 / 7.3 100S26R (AGC→AGAfnroxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (3/2);  new base T (44/51);  total (47/53)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01

Only 100 of 104 total aligned reads displayed.

GTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTC  >  NC_000913_3_pae_pgi/1399303‑1399590
                                                                                                                                                  |                                                                                                                                             
gTACCGGCGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAgctgtctg                                                                                                                                            <  4:723654/150‑1 (MQ=255)
gTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAgctgtctg                                                                                                                                            >  4:645534/1‑150 (MQ=255)
      gAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGAtgctg                                                                                                                                      <  2:56266/150‑1 (MQ=255)
      gAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGAtgctg                                                                                                                                      <  1:514161/150‑1 (MQ=255)
           gATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATc                                                                                                                                 <  3:497996/150‑1 (MQ=255)
             tGGCATAAGGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATCGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCt                                                                                                                               <  4:718229/150‑1 (MQ=255)
             tGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCt                                                                                                                               >  2:844950/1‑150 (MQ=255)
              ggCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTg                                                                                                                              <  1:1103796/150‑1 (MQ=255)
                cATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGc                                                                                                                            <  3:18563/150‑1 (MQ=255)
                     aGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAgctgtctg                                                                                                                                            <  2:461726/129‑1 (MQ=255)
                     aGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAgctgtctg                                                                                                                                            >  1:461726/1‑129 (MQ=255)
                     aGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATgg                                                                                                                       <  4:149039/150‑1 (MQ=255)
                     aGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATgg                                                                                                                       >  3:331521/1‑150 (MQ=255)
                      gCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGa                                                                                                                      <  1:470737/150‑1 (MQ=255)
                        gATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATa                                                                                                                    <  4:55909/150‑1 (MQ=255)
                          tttAAGCCCAGCACCATCCGTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCACTCCTGGCAATGGATAGc                                                                                                                  <  4:416655/150‑1 (MQ=255)
                              aGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACaa                                                                                                              >  1:116818/1‑150 (MQ=255)
                                ttCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAAcc                                                                                                            >  1:291916/1‑150 (MQ=255)
                                  catcaCCAGCCTTAAACAGCGTCTGGCCTGTCTGAATAGGCTTCTTCCGCTCAATGATAGTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGc                                                                                                          <  2:748564/150‑1 (MQ=255)
                                   atcaCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGcc                                                                                                         >  4:130763/1‑150 (MQ=255)
                                    tcaCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGca                                                                                                                 >  1:79028/1‑141 (MQ=255)
                                    tcaCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGca                                                                                                                 <  2:79028/141‑1 (MQ=255)
                                    tcaCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCa                                                                                                        <  1:288938/150‑1 (MQ=255)
                                     caCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAg                                                                                                       >  3:705728/1‑150 (MQ=255)
                                     caCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAg                                                                                                       <  1:138150/150‑1 (MQ=255)
                                      aCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGGTGCAATCCTGGCAATGGATAGCACAACCGCCAGa                                                                                                      >  1:889563/1‑150 (MQ=255)
                                      aCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTg                                                                                                                              >  3:728909/1‑126 (MQ=255)
                                      aCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTg                                                                                                                              <  4:728909/126‑1 (MQ=255)
                                      aCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGa                                                                                                      >  1:150814/1‑150 (MQ=255)
                                      aCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCACTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGa                                                                                                      <  2:1029388/150‑1 (MQ=255)
                                        cAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACt                                                                                                    <  2:1053957/150‑1 (MQ=255)
                                         aGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATc                                                                                                                                 <  3:616438/120‑1 (MQ=255)
                                         aGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATc                                                                                                                                 >  4:616438/1‑120 (MQ=255)
                                         aGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTg                                                                                                   <  3:172285/150‑1 (MQ=255)
                                          gCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTGGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAACCCTGGCAATGGATAGCACAACCGCCAGACTGa                                                                                                  <  4:610227/150‑1 (MQ=255)
                                                 aCAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGcc                                                                                           <  2:150814/150‑1 (MQ=255)
                                                 aCAGCGTCTGGCCTTTCTGAATACGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGcc                                                                                           >  4:703282/1‑150 (MQ=255)
                                                   aGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGt                                                                                         >  1:1116616/1‑150 (MQ=255)
                                                   aGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGt                                                                                         >  3:203506/1‑150 (MQ=255)
                                                    gCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGta                                                                                        <  3:43448/150‑1 (MQ=255)
                                                        cTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAAt                                                                                    >  4:33159/1‑150 (MQ=255)
                                                           gCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGa                                                                                                                                           >  3:727974/1‑92 (MQ=255)
                                                           gCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGa                                                                                                                                           <  4:727974/92‑1 (MQ=255)
                                                           gCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCg                                                                                 <  2:856602/150‑1 (MQ=255)
                                                           gCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCg                                                                                 >  2:970146/1‑150 (MQ=255)
                                                            ccTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGc                                                                                >  2:576723/1‑150 (MQ=255)
                                                              tttCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCtt                                                                              >  3:72418/1‑150 (MQ=255)
                                                              tttCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCtt                                                                              >  4:279245/1‑150 (MQ=255)
                                                              tttCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCtt                                                                              >  2:1201868/1‑150 (MQ=255)
                                                              tttCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAATGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCACCGTATAATTCGCtt                                                                              >  2:302113/1‑150 (MQ=255)
                                                              tttCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCGCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCtt                                                                              >  2:712889/1‑150 (MQ=255)
                                                               ttCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCttt                                                                             >  1:610759/1‑150 (MQ=255)
                                                                    aaTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGtata                                                                                      >  3:318135/1‑136 (MQ=255)
                                                                    aaTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGtata                                                                                      <  4:318135/136‑1 (MQ=255)
                                                                    aaTAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCgg                                                                        <  2:542615/150‑1 (MQ=255)
                                                                       aGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGAt                                                                     >  3:294990/1‑150 (MQ=255)
                                                                              ttcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGt                                                              >  3:275832/1‑150 (MQ=255)
                                                                                 cGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTg                                                           <  1:844950/150‑1 (MQ=255)
                                                                                 cGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTg                                                           <  4:658465/150‑1 (MQ=255)
                                                                                  gCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGc                                                          >  2:332701/1‑150 (MQ=255)
                                                                                       aTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGTAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAg                                                     <  2:695406/150‑1 (MQ=255)
                                                                                       aTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAg                                                     <  1:970146/150‑1 (MQ=255)
                                                                                         gATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTg                                                                                                   <  2:595717/102‑1 (MQ=255)
                                                                                         gATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTg                                                                                                   >  1:595717/1‑102 (MQ=255)
                                                                                         gATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGcc                                                   <  2:9581/150‑1 (MQ=255)
                                                                                           tattatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCCATCCTGGCAATGGATAGCACTACCGCCAGACTGAGTGCGCCGTATAATTCGCTTTTCCGGGCTCATAGGTCTGCTCATGCCGt                                                 <  2:500838/150‑1 (MQ=255)
                                                                                            attatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTa                                                <  2:64971/150‑1 (MQ=255)
                                                                                                  aaGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGat                                          <  1:215188/150‑1 (MQ=255)
                                                                                                  aaGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGat                                          >  1:58269/1‑150 (MQ=255)
                                                                                                   aGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATa                                                                 <  3:336824/126‑1 (MQ=255)
                                                                                                   aGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATa                                                                 >  4:336824/1‑126 (MQ=255)
                                                                                                      tGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAAt                                              >  3:81813/1‑142 (MQ=255)
                                                                                                      tGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATAttt                                      >  3:8978/1‑150 (MQ=255)
                                                                                                      tGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATACTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATAttt                                      <  2:863064/150‑1 (MQ=255)
                                                                                                      tGATCAAGCTCATCTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATAttt                                      <  4:648502/150‑1 (MQ=255)
                                                                                                          cAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAGTGCGGCGTATAATTCGCTTTTCCGGGATCATAGGGCTGCTCAAGTCGTAATTGATATTTGTCa                                  <  2:403633/150‑1 (MQ=255)
                                                                                                          cAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCa                                  >  4:14638/1‑150 (MQ=255)
                                                                                                            aGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAAt                                >  3:347039/1‑150 (MQ=255)
                                                                                                             gCTCATGCTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAAtt                               >  2:1213341/1‑150 (MQ=255)
                                                                                                              cTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAAttt                              <  4:203506/150‑1 (MQ=255)
                                                                                                               tCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGc                                                    <  4:683229/127‑1 (MQ=255)
                                                                                                               tCATGTTCGTTGAGTGAGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGc                                                    >  3:683229/1‑127 (MQ=255)
                                                                                                                cATGTTCGTTGAGTGTGACCGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTa                            <  4:665023/150‑1 (MQ=255)
                                                                                                                cATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTa                            <  2:777419/150‑1 (MQ=255)
                                                                                                                 aTGTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCGAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTaa                           <  2:844278/150‑1 (MQ=255)
                                                                                                                   gTTCGTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACa                         >  2:445452/1‑150 (MQ=255)
                                                                                                                       gTTGAGTGTGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCtt                     >  1:868829/1‑150 (MQ=255)
                                                                                                                            gtgtGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAg                >  4:51504/1‑150 (MQ=255)
                                                                                                                            gtgtGAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAg                <  4:705728/150‑1 (MQ=255)
                                                                                                                             tgtgAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAgg               <  1:576723/150‑1 (MQ=255)
                                                                                                                               tgAACGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGa             >  4:499587/1‑150 (MQ=255)
                                                                                                                                  aCGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGTGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCa          >  1:164843/1‑150 (MQ=255)
                                                                                                                                  aCGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCa          >  1:164908/1‑150 (MQ=255)
                                                                                                                                   cGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGt                                                              >  4:263009/1‑97 (MQ=255)
                                                                                                                                   cGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGt                                                              <  3:263009/97‑1 (MQ=255)
                                                                                                                                   cGGGATGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaa         <  1:730497/150‑1 (MQ=255)
                                                                                                                                       aTGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCaagtaa     <  4:275832/150‑1 (MQ=255)
                                                                                                                                        tGCAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGAaagtaag    >  3:700745/1‑150 (MQ=255)
                                                                                                                                          cAAAGCTGTCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTc  >  4:492201/1‑150 (MQ=255)
                                                                                                                                           aaaGCTGTCTGATGCTGCAATCCTGGCAATGGCTAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGc                                                                                <  4:109624/71‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
GTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTCGCTTTTCCGGGATCATAGGTCTGCTCAAGCCGTAATTGATATTTGTCAATTTTAACATCTTTTTAGGGAGCAAGTAAGTC  >  NC_000913_3_pae_pgi/1399303‑1399590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: