Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,159,090 C→T R201C (CGC→TGC)  oxyR → oxidative and nitrosative stress transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,159,0900CT97.9% 322.8 / ‑2.3 93R201C (CGC→TGC) oxyRoxidative and nitrosative stress transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (48/43);  total (49/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCG  >  NC_000913_3_pae_pgi/4158945‑4159235
                                                                                                                                                 |                                                                                                                                                  
cGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGa                                                                                                                                                >  1:700454/1‑150 (MQ=255)
 gAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGAt                                                                                                                                               <  2:700454/150‑1 (MQ=255)
         cattGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAAt                                                                                                                                       <  3:264615/150‑1 (MQ=255)
               aGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATTCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGttt                                                                                                                                 >  1:256146/1‑150 (MQ=255)
                gTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTc                                                                                                                                >  4:610606/1‑150 (MQ=255)
                   ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTTGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGt                                                                                                                             <  4:314082/150‑1 (MQ=255)
                   ccGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGt                                                                                                                             >  2:992003/1‑150 (MQ=255)
                     gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGttt                                                                                                                           <  3:234030/150‑1 (MQ=255)
                     gttgttTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGttt                                                                                                                           <  3:372663/150‑1 (MQ=255)
                             atgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCACTGGGTTTCTGTTTTGAAGCCg                                                                                                                   >  2:803938/1‑150 (MQ=255)
                              tgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGAAATGGGTTTCTGTTTTGATGCCgg                                                                                                                  <  4:336033/150‑1 (MQ=255)
                              tgaGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTAACGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgg                                                                                                                  >  3:680132/1‑150 (MQ=255)
                                aGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCgggg                                                                                                                >  1:921978/1‑150 (MQ=255)
                                 gCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTgtgc                                                                                                                                                  <  3:571336/115‑1 (MQ=255)
                                  ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCATGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCt                                                                                                                               <  1:663045/133‑1 (MQ=255)
                                  ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCt                                                                                                                               >  2:663045/1‑133 (MQ=255)
                                  ccAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCg                                                                                                              >  2:216374/1‑150 (MQ=255)
                                   cAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCgg                                                                                                             >  3:614606/1‑150 (MQ=255)
                                   cAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCgg                                                                                                             <  1:24537/150‑1 (MQ=255)
                                       gTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGa                                                                                                         >  2:1078955/1‑150 (MQ=255)
                                           cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGtttt                                                                                                                          >  4:108788/1‑129 (MQ=255)
                                           cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGtttt                                                                                                                          <  3:108788/129‑1 (MQ=255)
                                           cTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGAt                                                                                                     <  3:52538/150‑1 (MQ=255)
                                            tGGCTATCTATGAAGATCACCCGTGNGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGCTa                                                                                                    >  3:544208/1‑150 (MQ=255)
                                            tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                                                                    >  1:806470/1‑150 (MQ=255)
                                            tGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATa                                                                                                    >  2:1009104/1‑150 (MQ=255)
                                              gCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATaca                                                                                                  >  1:158167/1‑150 (MQ=255)
                                                 atctatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATacacac                                                                                               >  2:1058737/1‑150 (MQ=255)
                                                    tatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAAt                                                                                                                                       <  2:962845/107‑1 (MQ=255)
                                                    tatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAAt                                                                                                                                       >  1:962845/1‑107 (MQ=255)
                                                    tatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCgg                                                                                                             >  3:72937/1‑133 (MQ=255)
                                                    tatGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCgg                                                                                                             <  4:72937/133‑1 (MQ=255)
                                                     atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGttt                                                                                                                                 >  4:155860/1‑112 (MQ=255)
                                                     atGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGttt                                                                                                                                 <  3:155860/112‑1 (MQ=255)
                                                      tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                                                          >  1:1001200/1‑150 (MQ=255)
                                                      tGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcg                                                                                          >  2:280030/1‑150 (MQ=255)
                                                       gAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGa                                                                                                         <  2:907248/134‑1 (MQ=255)
                                                       gAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGa                                                                                                         >  1:907248/1‑134 (MQ=255)
                                                               cccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAg                                                                                 >  3:128807/1‑150 (MQ=255)
                                                                ccGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGc                                                                                >  1:77380/1‑150 (MQ=255)
                                                                 cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGATGCCGGGGAGGATGAAg                                                                                                       <  2:582786/126‑1 (MQ=255)
                                                                 cGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCGGGGGAGGATGAAg                                                                                                       >  1:582786/1‑126 (MQ=255)
                                                                     ggCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGa                                                                           <  4:148594/150‑1 (MQ=255)
                                                                      gCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGaa                                                                          >  3:246451/1‑150 (MQ=255)
                                                                           ccGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAActct                                                                     >  4:11114/1‑150 (MQ=255)
                                                                            cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgc                                                                                         <  2:576499/129‑1 (MQ=255)
                                                                            cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCcgc                                                                                         >  1:576499/1‑129 (MQ=255)
                                                                            cgcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTg                                                                    >  2:647439/1‑150 (MQ=255)
                                                                             gcgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTgc                                                                   <  1:290416/150‑1 (MQ=255)
                                                                              cgAATGCGTACGGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa                                                                                                            <  2:707785/108‑1 (MQ=255)
                                                                              cgAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGa                                                                                                            >  1:707785/1‑108 (MQ=255)
                                                                                  tGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGTGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGAGCAGCCTGGAAACTCTGCGCAAc                                                              <  2:393179/150‑1 (MQ=255)
                                                                                      tACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtgg                                                          >  4:601615/1‑150 (MQ=255)
                                                                                       aCCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGa                                                                                                                                                >  3:423777/1‑63 (MQ=255)
                                                                                       aCCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGa                                                                                                                                                <  4:423777/63‑1 (MQ=255)
                                                                                        ccGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtg                                                        <  3:91453/150‑1 (MQ=255)
                                                                                         cGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACAtggtgg                                                       <  1:1058737/150‑1 (MQ=255)
                                                                                          gATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggc                                                      <  3:204691/150‑1 (MQ=255)
                                                                                             ggCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcggc                                                   >  1:762633/1‑150 (MQ=255)
                                                                                              gCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa                                                  >  1:958529/1‑150 (MQ=255)
                                                                                              gCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCa                                                  >  4:25859/1‑150 (MQ=255)
                                                                                                 gttCTGGCGGGGGAAAAACTGCTGATGCTGGAAGATGGTCAGTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGACGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGt                                               <  2:158167/148‑1 (MQ=255)
                                                                                                  aTCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTa                                              <  2:958529/150‑1 (MQ=255)
                                                                                                       gCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCggg                                         >  1:32379/1‑150 (MQ=255)
                                                                                                        cAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGa                                        <  1:647439/150‑1 (MQ=255)
                                                                                                         aGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGAt                                       <  3:11114/150‑1 (MQ=255)
                                                                                                          ggggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa                                                               <  1:809284/125‑1 (MQ=255)
                                                                                                          ggggAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCaa                                                               >  2:809284/1‑125 (MQ=255)
                                                                                                             gAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACt                                   <  3:495150/150‑1 (MQ=255)
                                                                                                               aaaaCTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACttt                                 <  3:25859/150‑1 (MQ=255)
                                                                                                                 aaCTGCTGATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTAc                               <  2:921978/150‑1 (MQ=255)
                                                                                                                    tgctgATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGc                           <  1:280030/150‑1 (MQ=255)
                                                                                                                    tgctgATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGc                           >  4:252909/1‑150 (MQ=255)
                                                                                                                     gcggATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGcc                          <  4:680132/147‑1 (MQ=255)
                                                                                                                        gATGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGAAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgc                       >  1:804540/1‑150 (MQ=255)
                                                                                                                         aTGCTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAgcg                      >  4:500377/1‑150 (MQ=255)
                                                                                                                            cTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGc                                                                                                                     <  3:173536/53‑1 (MQ=255)
                                                                                                                            cTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGc                                                                                                                     >  4:173536/1‑53 (MQ=255)
                                                                                                                            cTGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctg                   <  2:77380/150‑1 (MQ=255)
                                                                                                                             tGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg                  <  1:875789/150‑1 (MQ=255)
                                                                                                                             tGGAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctgg                  <  1:120926/150‑1 (MQ=255)
                                                                                                                              ggAAGATGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCgctggc                 >  4:500535/1‑150 (MQ=255)
                                                                                                                                   aTGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcgg                                                    <  3:277596/111‑1 (MQ=255)
                                                                                                                                   aTGGTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTggcgg                                                    >  4:277596/1‑111 (MQ=255)
                                                                                                                                     ggTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccg          <  4:128807/150‑1 (MQ=255)
                                                                                                                                      gTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGTGCGGATGAAGATACACACTTTCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTCGCGGGAGGACGGTGCGTGCCGCGTTGGCTGTgccgc         >  2:335243/1‑150 (MQ=255)
                                                                                                                                      gTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgc         <  1:102650/150‑1 (MQ=255)
                                                                                                                                      gTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgc         >  2:557023/1‑150 (MQ=255)
                                                                                                                                      gTCACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTgccgc         <  3:610606/150‑1 (MQ=255)
                                                                                                                                       tCACTGTTTGTGCGATCAGACAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCtgt              >  1:479397/1‑144 (MQ=255)
                                                                                                                                       tCACTGTTTGTGCGATCAGACAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCtgt              <  2:479397/144‑1 (MQ=255)
                                                                                                                                        cACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAg                                             <  4:604418/113‑1 (MQ=255)
                                                                                                                                        cACTGTTTGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAg                                             >  3:604418/1‑113 (MQ=255)
                                                                                                                                             tttGTGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAgcg  >  1:821891/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CGAAGCATTCATTGAAGTGCCGTTGTTTGATGAGCCAATGTTGCTGGCTATCTATGAAGATCACCCGTGGGCGAACCGCGAATGCGTACCGATGGCCGATCTGGCAGGGGAAAAACTGCTGATGCTGGAAGATGGTCACTGTTTGCGCGATCAGGCAATGGGTTTCTGTTTTGAAGCCGGGGCGGATGAAGATACACACTTCCGCGCGACCAGCCTGGAAACTCTGCGCAACATGGTGGCGGCAGGTAGCGGGATCACTTTACTGCCAGCGCTGGCTGTGCCGCCGGAGCG  >  NC_000913_3_pae_pgi/4158945‑4159235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: