Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	202,398	C→T	R100C (CGC→TGC)	fabZ →	(3R)‑hydroxymyristol acyl carrier protein dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	202,398	0	C	T	100.0%	66.5 / NA	20	R100C (CGC→TGC)	fabZ	(3R)‑hydroxymyristol acyl carrier protein dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/11);  total (9/11)

AGGGCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTC  >  NC_000913_3_pae_pgi/202255‑202539                                                                                                                                                |                                                                                                                                              aGGGCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCa                                                                                                                                         >  2:9270/1‑150 (MQ=255)    gCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAgc                                                                                                                                      <  1:153432/150‑1 (MQ=255)    gCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAgc                                                                                                                                      <  1:9270/150‑1 (MQ=255)                         ttttCCCGGGTGTGCTGATTCTGGAAGCAATGGCAAAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATGGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATc                                                                                                                 <  2:88854/150‑1 (MQ=255)                                          aTTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTc                                                                                                >  1:173900/1‑150 (MQ=255)                                          aTTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTc                                                                                                >  4:31049/1‑150 (MQ=255)                                              tGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAgcgc                                                                                                                                    <  1:86154/110‑1 (MQ=255)                                              tGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAgcgc                                                                                                                                    >  2:86154/1‑110 (MQ=255)                                              tGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACtt                                                                                            >  1:109157/1‑150 (MQ=255)                                                  aGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGa                                                                                        >  2:154755/1‑150 (MQ=255)                                                                cAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCcgcg                                                                          <  4:3724/150‑1 (MQ=255)                                                                   cAGGTATTCTGGCGTGTAAAAGGGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCTTGGCGATCAAATGTTCATGGAAGTAAAAAAAAAAAAAACGCGCCGCGGcc                                                                       <  2:1262/150‑1 (MQ=255)                                                                                    aaaaGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAggg                                                      <  4:47747/150‑1 (MQ=255)                                                                                                            ggTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAagta                              <  1:154755/150‑1 (MQ=255)                                                                                                                        tactTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAATTCACTTTCTAAAAAACGCGCCGCGGCCTGACCCGCTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCa                  >  4:27207/1‑150 (MQ=255)                                                                                                                                  gTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGAtgt        <  2:70620/150‑1 (MQ=255)                                                                                                                                  gTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGAtgt        >  1:7448/1‑150 (MQ=255)                                                                                                                                        aCGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTc  <  2:173900/150‑1 (MQ=255)                                                                                                                                        aCGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTc  <  3:31049/150‑1 (MQ=255)                                                                                                                                        aCGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTc  >  1:189182/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                              AGGGCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTC  >  NC_000913_3_pae_pgi/202255‑202539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑