Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 202,398 C→T R100C (CGC→TGC)  fabZ → (3R)‑hydroxymyristol acyl carrier protein dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi202,3980CT99.3% 478.7 / ‑4.6 137R100C (CGC→TGC) fabZ(3R)‑hydroxymyristol acyl carrier protein dehydratase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (69/66);  total (71/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

Only 100 of 137 total aligned reads displayed.

TCCAGGGCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTC  >  NC_000913_3_pae_pgi/202252‑202539
                                                                                                                                                  |                                                                                                                                              
tcCAGGGCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCt                                                                                                                                             <  4:76590/150‑1 (MQ=255)
tcCAGGGCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCt                                                                                                                                             <  3:281169/150‑1 (MQ=255)
   aGGGCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCa                                                                                                                                          <  4:916754/150‑1 (MQ=255)
   aGGGCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCATCa                                                                                                                                          >  2:671912/1‑150 (MQ=255)
   aGGGCCATTTCCCTGGAAAACCGATTTACCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAACGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGATGCGTGCTTCa                                                                                                                                          >  2:712224/1‑150 (MQ=255)
     ggCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGGGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAg                                                                                                                                        >  3:797349/1‑150 (MQ=255)
         aTTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGcc                                                                                                                                    <  1:1252283/150‑1 (MQ=255)
              ccTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTc                                                                                                                               >  2:1535707/1‑150 (MQ=255)
                tGGAAAACCGATTTTCGCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGt                                                                                                                             >  2:856574/1‑150 (MQ=255)
                tGGAAAACCGATTTGCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGt                                                                                                                             <  2:1186049/150‑1 (MQ=255)
                 ggAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTg                                                                                                                            >  4:911136/1‑150 (MQ=255)
                  gAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGc                                                                                                                           >  2:459261/1‑150 (MQ=255)
                      aCCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTgg                                                                                                                       >  1:788919/1‑150 (MQ=255)
                       ccGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTggt                                                                                                                      >  1:10210/1‑149 (MQ=255)
                       ccGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGc                                                                                                                      <  2:1321392/150‑1 (MQ=255)
                                 cGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGcc                                                                                                                                    >  4:819428/1‑126 (MQ=255)
                                 cGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGcc                                                                                                                                    <  3:819428/126‑1 (MQ=255)
                                  gggTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGAt                                                                                                           <  3:839790/150‑1 (MQ=255)
                                   ggTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATc                                                                                                          <  2:170029/150‑1 (MQ=255)
                                   ggTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATc                                                                                                          <  3:568769/150‑1 (MQ=255)
                                         cTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGaa                                                                                                    <  3:368790/150‑1 (MQ=255)
                                           gATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGt                                                                                                  >  3:59363/1‑150 (MQ=255)
                                            aTTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTc                                                                                                 >  2:621165/1‑150 (MQ=255)
                                            aTTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTc                                                                                                 >  3:1036375/1‑150 (MQ=255)
                                                tGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGAt                                                                                                           >  3:470888/1‑136 (MQ=255)
                                                tGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGAt                                                                                                           <  4:470888/136‑1 (MQ=255)
                                                 ggAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGTCTGGCGATCAAATGATCATGGAAGTCACttt                                                                                            <  3:220327/150‑1 (MQ=255)
                                                 ggAAGCAATGGCACAGACAACAGGTATTATGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGGGAGCGGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCCAATGATCACGGAAGTCACttt                                                                                            >  2:875631/1‑150 (MQ=255)
                                                    aGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGa                                                                                        >  3:313080/1‑150 (MQ=255)
                                                      cAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGaaa                                                                                      >  1:908744/1‑150 (MQ=255)
                                                          ggCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTg                                                                                                                        <  3:782117/113‑1 (MQ=255)
                                                          ggCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTg                                                                                                                        >  4:782117/1‑113 (MQ=255)
                                                          ggCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAAc                                                                                  >  1:1182439/1‑150 (MQ=255)
                                                           gCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGaaaaa                                                                                    <  4:589945/147‑1 (MQ=255)
                                                           gCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAAcg                                                                                 >  1:890090/1‑150 (MQ=255)
                                                            cacaGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAAcgc                                                                                <  3:1039293/150‑1 (MQ=255)
                                                            cacaGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAAcgc                                                                                <  2:890090/150‑1 (MQ=255)
                                                                ggCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGcgccg                                                                            >  2:1073907/1‑150 (MQ=255)
                                                                 gCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGcgccgc                                                                           >  2:74030/1‑150 (MQ=255)
                                                                 gCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGcgccgc                                                                           >  1:1502876/1‑150 (MQ=255)
                                                                      agccaTTCTGGGCTTGAAAAGCGTAGTAAAACTGGAACCGGGTGAGCTGAACTACTTCGTTGCTATTGACGAAGCGGGCTTCAAGCGCCCGGTCGTGCCTTGTGATCAAATGATCATGGAACTCACTATCGAAAAAATGCTCCGCGGCCt                                                                      >  2:1241907/5‑150 (MQ=255)
                                                                        gTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAATTCACTTTCGAAAAAACGAACCGAGGACTGa                                                                    >  3:619845/1‑150 (MQ=255)
                                                                           ttCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGAccc                                                                 >  4:630650/1‑150 (MQ=255)
                                                                              tGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGtt                                                              >  2:409597/1‑150 (MQ=255)
                                                                              tGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGtt                                                              <  1:1629052/150‑1 (MQ=255)
                                                                              tGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGtt                                                              <  1:1535841/150‑1 (MQ=255)
                                                                                gCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGtttt                                                            <  2:908744/150‑1 (MQ=255)
                                                                                 cGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTa                                                           <  3:911136/150‑1 (MQ=255)
                                                                                  gTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTaa                                                          <  2:353257/150‑1 (MQ=255)
                                                                                  gTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTaa                                                          >  1:523358/1‑150 (MQ=255)
                                                                                   tttAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATgg                                                                                                      >  1:1008336/1‑106 (MQ=255)
                                                                                   tttAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATgg                                                                                                      <  2:1008336/106‑1 (MQ=255)
                                                                                   tttAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTaaa                                                         <  2:1548663/150‑1 (MQ=255)
                                                                                   tttAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTaaa                                                         <  4:797349/150‑1 (MQ=255)
                                                                                     tAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGTGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAgg                                                       <  2:10210/150‑1 (MQ=255)
                                                                                      aaaaGCGTAGGAAAACTGGAACCGGGTGAGCTTTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAggg                                                      <  3:1033165/150‑1 (MQ=255)
                                                                                        aaGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCt                                                                                                                                             <  4:428055/62‑1 (MQ=255)
                                                                                        aaGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCt                                                                                                                                             >  3:428055/1‑62 (MQ=255)
                                                                                        aaGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGt                                                    >  4:385271/1‑150 (MQ=255)
                                                                                         aGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGtt                                                   >  3:295299/1‑150 (MQ=255)
                                                                                          gCGTAGGAAGACTGGACCCGGGTGAGCTGGAGTACTTCGCTAGTATTGCCGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGGAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTg                                                  <  4:313080/150‑1 (MQ=255)
                                                                                          gCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGt                                                                                                                             <  1:340728/76‑1 (MQ=255)
                                                                                          gCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGt                                                                                                                             >  2:340728/1‑76 (MQ=255)
                                                                                           cGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGc                                                 <  1:746600/150‑1 (MQ=255)
                                                                                              aGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGctct                                              <  2:523358/150‑1 (MQ=255)
                                                                                                gAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTaaa                                                         >  1:177912/1‑137 (MQ=255)
                                                                                                gAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGctc                                               <  2:255959/147‑1 (MQ=255)
                                                                                                gAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGctc                                               >  1:255959/1‑147 (MQ=255)
                                                                                                gAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTgg                                            <  2:426055/150‑1 (MQ=255)
                                                                                                gAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTgg                                            >  1:559307/1‑150 (MQ=255)
                                                                                                gAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTgg                                            >  3:558309/1‑150 (MQ=255)
                                                                                                 aaaaCTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGt                                           <  1:856574/150‑1 (MQ=255)
                                                                                                 aaaaCTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGt                                           <  1:1535707/150‑1 (MQ=255)
                                                                                                 aaaaCTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGt                                           <  2:736264/150‑1 (MQ=255)
                                                                                                       ggAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGAAACTAACGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATgg                                     <  4:304919/150‑1 (MQ=255)
                                                                                                        gAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGt                                    >  1:704305/1‑150 (MQ=255)
                                                                                                        gAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTCTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGGTCTGGTCGATGGt                                    >  2:1088960/1‑150 (MQ=255)
                                                                                                          aCCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTc                                                                                                                                           >  1:34603/1‑46 (MQ=255)
                                                                                                              ggTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAagta                              <  4:1009316/150‑1 (MQ=255)
                                                                                                                   gCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCTCTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTg                         <  3:541453/150‑1 (MQ=255)
                                                                                                                   gCTGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTg                         <  3:425025/150‑1 (MQ=255)
                                                                                                                     tGTACTACTTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCg                       <  4:1036375/150‑1 (MQ=255)
                                                                                                                          tactTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAAcg                                                                                 >  2:1562562/1‑87 (MQ=255)
                                                                                                                          tactTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCa                  >  4:1028413/1‑150 (MQ=255)
                                                                                                                          tactTCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCa                  >  3:742485/1‑150 (MQ=255)
                                                                                                                             ttCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGaa                                                                                                    <  2:633162/66‑1 (MQ=255)
                                                                                                                             ttCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGc                                                 <  2:983740/116‑1 (MQ=255)
                                                                                                                             ttCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACg               >  4:864895/1‑150 (MQ=255)
                                                                                                                             ttCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACg               >  1:656611/1‑150 (MQ=255)
                                                                                                                              tCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTgg                                                                                                                       <  3:85893/46‑1 (MQ=255)
                                                                                                                              tCGCTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTgg                                                                                                                       >  4:85893/1‑46 (MQ=255)
                                                                                                                                 cTGGTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACgatga           >  4:270088/1‑150 (MQ=255)
                                                                                                                                  tGGTATTGAACAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGTGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACgatgat          <  1:1241907/150‑1 (MQ=255)
                                                                                                                                    gTATTGACGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGAtgt        <  1:1099426/150‑1 (MQ=255)
                                                                                                                                          aCGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCt   >  3:912130/1‑150 (MQ=255)
                                                                                                                                          aCGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGAt                                       >  2:494465/1‑113 (MQ=255)
                                                                                                                                          aCGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGAt                                       <  1:494465/113‑1 (MQ=255)
                                                                                                                                          aCGAAGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCATCGATGATGTGTGCTc  <  2:665465/150‑1 (MQ=255)
                                                                                                                                              aGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAag                                >  1:737211/1‑116 (MQ=255)
                                                                                                                                              aGCGTGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAag                                <  2:737211/116‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                              
TCCAGGGCCATTTCCCTGGAAAACCGATTTTCCCGGGTGTGCTGATTCTGGAAGCAATGGCACAGGCAACAGGTATTCTGGCGTTTAAAAGCGTAGGAAAACTGGAACCGGGTGAGCTGTACTACTTCGCTGGTATTGACGAAGCGCGCTTCAAGCGCCCGGTCGTGCCTGGCGATCAAATGATCATGGAAGTCACTTTCGAAAAAACGCGCCGCGGCCTGACCCGTTTTAAAGGGGTTGCTCTGGTCGATGGTAAAGTAGTTTGCGAAGCAACGATGATGTGTGCTC  >  NC_000913_3_pae_pgi/202252‑202539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: