Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,398,976 C→A R184L (CGC→CTC)  fnr ← oxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,398,9760CA95.6% 386.1 / 7.1 114R184L (CGC→CTC) fnroxygen‑sensing anaerobic growth regulon transcriptional regulator FNR; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (61/48);  total (63/51)
Fisher's exact test for biased strand distribution p-value = 6.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 114 total aligned reads displayed.

TGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCAT  >  NC_000913_3_pae_pgi/1398830‑1399121
                                                                                                                                                  |                                                                                                                                                 
  gTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTgg                                                                                                                                              <  2:667709/150‑1 (MQ=255)
    gATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGc                                                                                                                                            >  2:8649/1‑150 (MQ=255)
          tttACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAacg                                                                                                                                      >  1:1146621/1‑150 (MQ=255)
            tACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAacgac                                                                                                                                    <  1:8649/150‑1 (MQ=255)
                      tGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGtt                                                                                                                          >  2:1072751/1‑150 (MQ=255)
                         cAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTa                                                                                                                       >  1:525443/1‑150 (MQ=255)
                         cAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTa                                                                                                                       >  1:350830/1‑150 (MQ=255)
                            cATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGAt                                                                                                                    <  1:1072751/150‑1 (MQ=255)
                            cATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGAt                                                                                                                    <  1:627473/150‑1 (MQ=255)
                            cATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGAt                                                                                                                    >  1:944168/1‑150 (MQ=255)
                            cATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGAt                                                                                                                    <  2:682806/150‑1 (MQ=255)
                            cATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGAt                                                                                                                    >  1:1075409/1‑150 (MQ=255)
                               gCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCGCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGaa                                                                                                                 <  2:676289/150‑1 (MQ=255)
                               gCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGaa                                                                                                                 <  4:589778/150‑1 (MQ=255)
                                ccGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCTTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAAt                                                                                                                <  1:15427/150‑1 (MQ=255)
                                ccGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAAt                                                                                                                >  1:404190/1‑150 (MQ=255)
                                   cTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCa                                                                                                             <  3:949391/150‑1 (MQ=255)
                                      ttCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGcc                                                                                                          <  2:525443/150‑1 (MQ=255)
                                         tGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGa                                                                                                       >  3:16601/1‑150 (MQ=255)
                                         tGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGa                                                                                                       >  3:927282/1‑150 (MQ=255)
                                           gAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACgg                                                                                                                                  <  1:499493/121‑1 (MQ=255)
                                           gAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACg                                                                                                     >  4:797104/1‑150 (MQ=255)
                                             aGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGtt                                                                                                   >  3:962917/1‑150 (MQ=255)
                                              gCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTc                                                                                                  >  3:1018495/1‑150 (MQ=255)
                                                gACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCt                                                                                                >  2:23187/1‑150 (MQ=255)
                                                 aCCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTc                                                                                               <  3:710313/150‑1 (MQ=255)
                                                  cccAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCg                                                                                              <  3:357844/150‑1 (MQ=255)
                                                   ccAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGa                                                                                                                     <  2:850964/126‑1 (MQ=255)
                                                   ccAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGa                                                                                                                     >  1:850964/1‑126 (MQ=255)
                                                    cagcagACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGc                                                                                            <  1:1331490/150‑1 (MQ=255)
                                                     agcagACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAacga                                                                                                                                     >  4:420116/1‑108 (MQ=255)
                                                       cagACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCAtt                                                                                         >  1:1061120/1‑150 (MQ=255)
                                                            ggCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGGTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTttct                                                                                    >  2:1339572/1‑150 (MQ=255)
                                                             gCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGa                                                                                                                  <  2:783381/119‑1 (MQ=255)
                                                             gCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGa                                                                                                                  >  1:783381/1‑119 (MQ=255)
                                                              cTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTttcttc                                                                                  <  1:23187/150‑1 (MQ=255)
                                                              cTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTttctt                                                                                   <  3:15404/149‑1 (MQ=255)
                                                              cTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTttctt                                                                                   >  4:15404/1‑149 (MQ=255)
                                                               tGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCg                                                                                 <  2:944168/150‑1 (MQ=255)
                                                               tGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCg                                                                                 >  4:621048/1‑150 (MQ=255)
                                                                gATGGTTTCTACCGTCAGGCCCATATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGa                                                                                <  3:797104/150‑1 (MQ=255)
                                                                 aTGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGac                                                                               >  3:253884/1‑150 (MQ=255)
                                                                  tGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGaca                                                                              >  3:1036062/1‑150 (MQ=255)
                                                                    gcttCTACCGTCAGGCCCAGATAGTTCCCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGACGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGactac                                                                            <  4:328027/148‑1 (MQ=255)
                                                                    gTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGc                                                                                                                                            <  3:374079/86‑1 (MQ=255)
                                                                    gTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGc                                                                                                                                            >  4:374079/1‑86 (MQ=255)
                                                                    gTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGAGAGAAGCCGAGTTTGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGacaac                                                                            >  4:423461/1‑150 (MQ=255)
                                                                          aCCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAgg                                                                      >  3:602135/1‑150 (MQ=255)
                                                                            cGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGAt                                                                    >  4:837833/1‑150 (MQ=255)
                                                                             gTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATc                                                                   <  2:842551/150‑1 (MQ=255)
                                                                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATc                                                                   >  2:652595/1‑145 (MQ=255)
                                                                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTc                                                              >  1:550992/1‑150 (MQ=255)
                                                                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTc                                                              >  2:1070256/1‑150 (MQ=255)
                                                                                  gCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTc                                                              >  3:590872/1‑150 (MQ=255)
                                                                                    ccAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTg                                                                                                                                               >  4:709400/1‑67 (MQ=255)
                                                                                    ccAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTg                                                                                                                                               <  3:709400/67‑1 (MQ=255)
                                                                                     cAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTg                                                           >  1:427617/1‑150 (MQ=255)
                                                                                        aTAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATc                                                        <  3:595496/150‑1 (MQ=255)
                                                                                            ttACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAGTGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCt                                                    >  2:26414/1‑150 (MQ=255)
                                                                                                cGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGa                                                >  1:1150702/1‑150 (MQ=255)
                                                                                                  atatCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGAtt                                              <  1:1627415/150‑1 (MQ=255)
                                                                                                  atatCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAACAGGATCATGTCCTGATCGCCTTTGAtt                                              >  3:926024/1‑150 (MQ=255)
                                                                                                   tatCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAacgacg                                                                                                                                   >  2:502160/1‑64 (MQ=255)
                                                                                                   tatCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAacgacg                                                                                                                                   <  1:502160/64‑1 (MQ=255)
                                                                                                      cGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCAc                                          <  2:1497544/150‑1 (MQ=255)
                                                                                                        ccACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGtt                                                                                                                          <  4:730322/68‑1 (MQ=255)
                                                                                                        ccACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGtt                                                                                                                          >  3:730322/1‑68 (MQ=255)
                                                                                                        ccACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCg                                        <  2:350830/150‑1 (MQ=255)
                                                                                                          aCGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCAc                                          <  1:198873/146‑1 (MQ=255)
                                                                                                          aCGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCAc                                          >  2:198873/1‑146 (MQ=255)
                                                                                                          aCGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCt                                      <  2:371466/150‑1 (MQ=255)
                                                                                                           cGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCtc                                     <  3:990474/150‑1 (MQ=255)
                                                                                                                 aTCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATg                                                                                                               <  1:1675048/70‑1 (MQ=255)
                                                                                                                 aTCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGa                               >  1:919042/1‑150 (MQ=255)
                                                                                                                 aTCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGa                               >  4:349737/1‑150 (MQ=255)
                                                                                                                  tCGTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGAc                              >  3:390207/1‑150 (MQ=255)
                                                                                                                    gTCAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGc                            >  4:159704/1‑150 (MQ=255)
                                                                                                                      cAGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAGTGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGcat                          <  1:26414/150‑1 (MQ=255)
                                                                                                                       aGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGcatc                         >  2:486084/1‑150 (MQ=255)
                                                                                                                       aGGCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGcatc                         >  3:433444/1‑150 (MQ=255)
                                                                                                                       aGGCGGAATTCAAGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGACCATGTCCTGACCGCCTTTGATTTCCCCGCTCATCAGACGcatc                         <  2:1011329/150‑1 (MQ=255)
                                                                                                                        ggCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGcatca                        >  1:1420230/1‑150 (MQ=255)
                                                                                                                         gCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGcatcat                       >  2:1501290/1‑150 (MQ=255)
                                                                                                                         gCGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGGTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGcatcat                       >  4:494229/1‑150 (MQ=255)
                                                                                                                          cGGAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACACatcatc                      >  3:603152/1‑150 (MQ=255)
                                                                                                                            gAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAAt                                                                                                                <  3:533033/58‑1 (MQ=255)
                                                                                                                            gAATTCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATctg                    <  2:404190/150‑1 (MQ=255)
                                                                                                                               ttCACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATctgctg                 >  2:1303271/1‑150 (MQ=255)
                                                                                                                                 cACGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCCGGCTCATGTCATGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGAc               >  3:622802/1‑150 (MQ=255)
                                                                                                                                  aCGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTttctt                                                                                   >  4:337689/1‑81 (MQ=255)
                                                                                                                                  aCGAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTttctt                                                                                   <  3:337689/81‑1 (MQ=255)
                                                                                                                                    gAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcagc                                                                         <  2:899380/89‑1 (MQ=255)
                                                                                                                                    gAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAAcagc                                                                         >  1:899380/1‑89 (MQ=255)
                                                                                                                                    gAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGc                                       <  4:867549/123‑1 (MQ=255)
                                                                                                                                    gAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGc                                       >  3:867549/1‑123 (MQ=255)
                                                                                                                                    gAGGGGAGAAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCa            >  1:1325053/1‑150 (MQ=255)
                                                                                                                                         gagaAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTc       <  3:881342/150‑1 (MQ=255)
                                                                                                                                         gagaAGCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTc       >  3:764830/1‑150 (MQ=255)
                                                                                                                                             aGCCGAGTTGGGCAAAACGACGGGACAGGATGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCa   <  2:1061519/150‑1 (MQ=255)
                                                                                                                                              gCCGAGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCAt  >  3:230449/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGGTGATGTATTTACCTTTGACTGCCAGCATGCCGCTTTTCTGGAAGCGACCCAGCAGACGGCTGATGGTTTCTACCGTCAGGCCCAGATAGTTACCGATATCGCCACGAGTCATCGTCAGGCGGAATTCACGAGGGGAGAAGCCGCGTTGGGCAAAACGACGGGACAGGTTGTAGATGAATGCAGCCAGACGTTCCTCGGCATTTTTCTTCGACAACAGCAGGATCATGTCCTGATCGCCTTTGATTTCACCGCTCATCAGACGCATCATCTGCTGACGCAGATTCGGCAT  >  NC_000913_3_pae_pgi/1398830‑1399121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: