Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi526,8000AG26.4% 221.8 / 79.2 130E1180E (GAA→GAGrhsDRhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (35/60);  new base G (13/21);  total (49/81)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.08e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 131 total aligned reads displayed.

AGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTAT  >  NC_000913_3_pae_pgi/526655‑526944
                                                                                                                                                 |                                                                                                                                                
  tgggggCTGACGGGGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatga                                                                                                                                             <  2:283984/146‑1 (MQ=11)
  tGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatga                                                                                                                                             <  2:1186878/149‑1 (MQ=255)
   gcgggcggACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                            <  2:1326944/149‑1 (MQ=11)
   gCGGGCTGATGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTatgat                                                                                                                                            <  2:1136389/149‑1 (MQ=11)
       gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAt                                                                                                                                        >  2:455059/1‑149 (MQ=255)
       gCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAt                                                                                                                                        <  1:645976/149‑1 (MQ=255)
        cTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATATACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATg                                                                                                                                       <  1:1253096/149‑1 (MQ=11)
        cTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATg                                                                                                                                       <  1:129200/149‑1 (MQ=255)
         tGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAAtag                                                                                                                                      >  2:841052/1‑147 (MQ=255)
          ggcGGTGGAGCAACGGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                     <  1:651840/147‑1 (MQ=11)
          gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTTCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                     <  1:13852/149‑1 (MQ=255)
          gACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATggg                                                                                                                                     <  1:327675/149‑1 (MQ=255)
           aCGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgggg                                                                                                                                    <  1:843808/149‑1 (MQ=255)
             ggTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCa                                                                                                                                  >  1:1595039/1‑149 (MQ=255)
              gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                     <  1:1161898/145‑1 (MQ=255)
              gTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATggg                                                                                                                                     >  2:1161898/1‑145 (MQ=255)
              gTGGAGCAACTGGCCAGACAGGTGAAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGGCTGCCGCTGGCGCTTATCAGCGAAGACGGCAATGCGGCGTGGAGCGCGGAATATGCTGATTGCGGCaa                                                                                                                                 >  1:480958/1‑149 (MQ=17)
               tGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAAc                                                                                                                                <  1:1389484/149‑1 (MQ=255)
                ggAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAAcc                                                                                                                               >  1:226053/1‑149 (MQ=255)
                 gAGGAACGGGCCAGAGAGGTGGAAGCGGAATAGACACCGGCGCGAAAAGTTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCa                                                                                                                              <  2:231028/149‑1 (MQ=14)
                 gAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCCGGGCCGTGTCAGCGAAGACGGCAATCCGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCa                                                                                                                              >  1:376394/1‑149 (MQ=11)
                   gCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCAGCTTTATCACTGCGACCACCGGGGACTGCCGCCGGCGCTTATCAGCGAAGAGCGCAATACGGCGAGACGCGCGGACTATGAGGAAGGGGGCAACCAGc                                                                                                                            >  2:1562424/1‑149 (MQ=14)
                    ccagtGGCGAGACAGGTGGAGCGGGAATACACGCCTGCGCGAAAAGCTCATCTTAATCACTGCGACCACTGGGGACTTCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTTGAGCGCGGAATATGATTAATGGGGCAACCAGCt                                                                                                                           <  2:367719/145‑1 (MQ=17)
                    cAACTGGCCGGACAGGTGGAGCCGGAATACACCCCGGCGGGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCt                                                                                                                           <  2:363484/149‑1 (MQ=11)
                       cttgCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTaa                                                                                                                        <  1:743052/146‑1 (MQ=11)
                        tGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCGTATCAGCGACGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAAt                                                                                                                       <  2:1602504/149‑1 (MQ=255)
                         ggCCAGGCAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGGGCGCGGAATATGATGAATGGGGCAACCAGCTTAATg                                                                                                                      >  1:1218877/1‑149 (MQ=255)
                         ggCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATg                                                                                                                      <  1:1600229/149‑1 (MQ=255)
                          gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                     <  1:956002/149‑1 (MQ=255)
                          gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGTGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                     <  1:792805/149‑1 (MQ=255)
                          gCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGATGGCGCTTATCAGCGAAGAAGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATga                                                                                                                     >  1:332346/1‑149 (MQ=11)
                            cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTTCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGGATGGGGCAACCAGCTTGCTgagg                                                                                                                   >  1:1055843/1‑149 (MQ=11)
                            cAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                   <  1:472526/149‑1 (MQ=255)
                                cAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGaa                                                                                                               >  1:1344248/1‑149 (MQ=255)
                                    tGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGGATGGGGGAACCAGCTTACTgaggaaggtctt                                                                                                           >  2:1534612/1‑142 (MQ=17)
                                     ggAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGc                                                                                                          >  1:1363659/1‑149 (MQ=255)
                                       aGCCGGAATACATACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcat                                                                                                        <  2:697452/149‑1 (MQ=11)
                                        gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatc                                                                                                       >  1:924602/1‑149 (MQ=255)
                                        gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                     <  2:43284/135‑1 (MQ=255)
                                        gCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATga                                                                                                                     >  1:43284/1‑135 (MQ=255)
                                         cgggAATACACACCGGCGGGAAAAGTTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatca                                                                                                      <  2:438509/147‑1 (MQ=255)
                                         ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATCCGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTGATGAGGAGAACCCGcatca                                                                                                      >  2:974658/1‑149 (MQ=11)
                                         ccGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGCAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatca                                                                                                      >  2:1336327/1‑149 (MQ=255)
                                           ggAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtg                                                                                                    <  1:760975/149‑1 (MQ=255)
                                            gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacct                                                                                                   <  1:1474676/149‑4 (MQ=255)
                                            gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                   >  2:809702/1‑133 (MQ=255)
                                            gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATgagg                                                                                                                   <  1:809702/133‑1 (MQ=255)
                                            gAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                   <  2:549654/149‑1 (MQ=255)
                                            gAACACACACCGGCGCGAAAAGCTCATCTTTACCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGGGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                   <  1:1348820/149‑1 (MQ=255)
                                               tACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTa                                                                                                <  2:1114307/149‑1 (MQ=255)
                                                acacacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATCAATGGGGCAACCAGCTTAATGAGGAGAACCCGcatcacctgcac                                                                                               >  2:383359/1‑142 (MQ=11)
                                                  acacCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCa                                                                                             >  2:1006446/1‑149 (MQ=255)
                                                    acCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                      <  1:1466153/106‑1 (MQ=11)
                                                    acCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATgg                                                                                                                                      >  2:1466153/1‑106 (MQ=255)
                                                          gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                     <  1:1336327/149‑1 (MQ=255)
                                                          gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                     <  1:455059/149‑1 (MQ=255)
                                                          gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                     >  1:1498521/1‑149 (MQ=255)
                                                          gcgAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGGGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTAcc                                                                                     <  1:1444612/149‑1 (MQ=255)
                                                              aaaGCTCACCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCt                                                                                 <  1:603768/149‑1 (MQ=255)
                                                                  cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                       >  1:272453/1‑107 (MQ=255)
                                                                  cTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGCGCGGGGAGTATGATGAATGGGGCAACCAGCTTAAt                                                                                                                       <  2:272453/107‑1 (MQ=11)
                                                                   tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGCTGAATGGGGCAACCAGATTAATTAGGAGAACTCGCACAATGTGAAGCAGCAGTACCGTCtccagc                                                                            >  2:172255/1‑144 (MQ=17)
                                                                   tCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAg                                                                            >  1:777362/1‑149 (MQ=255)
                                                                     aTCTTTATCACTGCTACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCACGTGTATCAGCCGTACCGTCTGCCAggg                                                                          <  2:1581295/149‑1 (MQ=255)
                                                                        tttATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcag                                                                       >  2:615568/1‑149 (MQ=255)
                                                                           aTCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcag                                                                    <  2:1070642/149‑1 (MQ=255)
                                                                             cACTGCGACCAACGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGgcagcagca                                                                  >  1:993980/1‑149 (MQ=255)
                                                                                   gACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                            <  1:59371/149‑1 (MQ=255)
                                                                                   gACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                            <  1:1132943/149‑1 (MQ=255)
                                                                                   gACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCAtgatga                                                            <  1:1382914/149‑1 (MQ=255)
                                                                                      cactGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATatg                                                                                                                                              >  2:1516457/1‑64 (MQ=255)
                                                                                       accGGGGACTGCCGCTGGCGCTTATCAGCGAAGCAGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGATTAATGAGGAGAACCCGCATCAAGTGTATCAGACGTACCGGCTGCCAGGGCACAAGCATGATAAGGaa                                                        >  2:100041/1‑149 (MQ=17)
                                                                                           ggggCTGCCGCTGGCGCTTATCAGCGAGGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAg                                                    <  1:245950/149‑1 (MQ=255)
                                                                                           gggACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGt                                                                                   <  1:705654/118‑1 (MQ=255)
                                                                                           gagACGGCCGCGGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAg                                                    <  2:1053760/147‑1 (MQ=255)
                                                                                             gACTGGCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                  <  2:741511/149‑1 (MQ=255)
                                                                                             gACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGTGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAggg                                                  <  1:1259793/149‑1 (MQ=255)
                                                                                             gACAGCCGGTGGCGCTTATCAGCGACGACGGGAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAAGGCAGCAGCATGATGAGTAATCAgtg                                                  <  2:684397/149‑3 (MQ=17)
                                                                                                tggcGCTGGCGCCTATCAGCGAAGACGGCAATACGGGGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTg                                               <  2:1448749/146‑1 (MQ=255)
                                                                                                tGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCGGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGGAGCAGCATGATGAGGAATAAggagtg                                               >  1:429196/1‑145 (MQ=255)
                                                                                                  ccGTTGGCGCTTATCAGCGAAGACGGGAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGACAACCCGCATCACGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCGTGTTGAGGAAGCAGGGCTGTa                                             <  2:553425/149‑1 (MQ=17)
                                                                                                  ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAAccc                                                                                                            >  1:1412338/1‑86 (MQ=255)
                                                                                                  ccGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                             >  1:1448749/1‑149 (MQ=255)
                                                                                                  ccGCTGGCGCCTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTa                                             >  1:1568006/1‑149 (MQ=255)
                                                                                                         cgcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAc                                      <  1:290365/149‑1 (MQ=255)
                                                                                                          gcTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAAcc                                     >  2:1309710/1‑149 (MQ=255)
                                                                                                              aTCAGCGAGGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                   >  2:998629/1‑83 (MQ=255)
                                                                                                              aTCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCAtgt                                                                                                   <  1:998629/83‑1 (MQ=255)
                                                                                                                  gCGAAGACGGCAATACGGCGTGGAGCGCGAAATCCTAGGAATGGGGCAACCAGCTTAATGAGGCGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTGTAACCGTCACCgg                             >  2:349776/1‑149 (MQ=17)
                                                                                                                           ggaagaCGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGAt                    <  2:1434572/144‑1 (MQ=255)
                                                                                                                            cAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATc                   <  1:894584/149‑1 (MQ=255)
                                                                                                                                  ggCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGc             >  2:979553/1‑149 (MQ=255)
                                                                                                                                   gCGTCGAACGCGGAATATGATGAATAGGGAAACGAGCTTAACGAAGACAACCCCCATCATGTGTACCAGCCCTACCGTCGACCATGGCAGCAGCATGATGAGGAATCAGGGCTGAACTAAAACCGTCACCGGTACTACGATCCGTCGCa            <  2:1557496/149‑1 (MQ=255)
                                                                                                                                    cGTGGAGCGCGTAATATGATGAACGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTTTATCAGCCGTACCTTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAg           <  1:1137471/149‑1 (MQ=255)
                                                                                                                                    cGTGGAGCGAGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCAGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAg           <  1:546759/149‑1 (MQ=255)
                                                                                                                                     gTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAgg          <  1:1059149/149‑1 (MQ=255)
                                                                                                                                       ggAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAgggg        <  1:759766/149‑1 (MQ=255)
                                                                                                                                          gcgcGGAATATGATGAATTGCGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGCAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCgg     <  1:1061889/149‑1 (MQ=255)
                                                                                                                                            gcgGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGta   >  2:530261/1‑149 (MQ=255)
                                                                                                                                             cgGAATAGGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGtat  <  1:346309/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGTGCGGGCTGACGGTGGAGCAACTGGCCAGACAGGTGGAGCCGGAATACACACCGGCGCGAAAAGCTCATCTTTATCACTGCGACCACCGGGGACTGCCGCTGGCGCTTATCAGCGAAGACGGCAATACGGCGTGGAGCGCGGAATATGATGAATGGGGCAACCAGCTTAATGAGGAGAACCCGCATCATGTGTATCAGCCGTACCGTCTGCCAGGGCAGCAGCATGATGAGGAATCAGGGCTGTACTATAACCGTCACCGGTACTACGATCCGTTGCAGGGGCGGTAT  >  NC_000913_3_pae_pgi/526655‑526944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: