Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913_3_pae_pgi	4,399,663	T→G	F39C (TTT→TGT)	hfq →	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913_3_pae_pgi	4,399,663	0	T	G	100.0%	219.1 / NA	65	F39C (TTT→TGT)	hfq	global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/38);  total (27/38)

AGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAG  >  NC_000913_3_pae_pgi/4399517‑4399807                                                                                                                                                   |                                                                                                                                                 aGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAGTCTTTACAAGATTCGTTACTGAACGCACTGCGTAGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTtgtg                                                                                                                                                >  1:1512423/1‑149 (MQ=255) aGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAGATCGAGTCTtgtg                                                                                                                                                >  1:763655/1‑149 (MQ=255)  gTACAAATTAGCATATAAGGAAAAGAGAGAATTGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTATATTTAGTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGa                                                                                                                                               <  2:1077316/149‑1 (MQ=255)   tACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAACATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGAt                                                                                                                                              >  1:1061313/1‑149 (MQ=255)    aCAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATc                                                                                                                                             <  2:632268/149‑1 (MQ=255)     cAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCa                                                                                                                                            <  2:1607631/149‑1 (MQ=255)          aacgagataAGGAAAAGAGAGAAGCGCTAAGGGGCAATCTTTACAAGAGTCGTGCCGGAACGCACTGCGTCGGGAACGTGTTCGAGTTTCTATTTATTTGGTGAATGGTAGTACGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCg                                                                                                                                       <  2:933158/143‑1 (MQ=255)           aggatatAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGt                                                                                                                                      <  2:905218/146‑1 (MQ=255)           aGCATATAAGGAAAAGAGAGGATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGATCGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGt                                                                                                                                      <  2:1313481/149‑1 (MQ=255)           aGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGt                                                                                                                                      <  1:1324504/149‑1 (MQ=255)              atatAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAGTCGAGTCTTGTGATCAGTTCGTGAt                                                                                                                                   >  1:1586130/1‑149 (MQ=255)              atatAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGAt                                                                                                                                   >  1:1378433/1‑149 (MQ=255)                ataAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATcc                                                                                                                                 >  2:584487/1‑149 (MQ=255)                        aaGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGaaa                                                                                                                         <  1:1352536/149‑1 (MQ=255)                            gagaATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAaca                                                                                                                     <  1:584487/149‑1 (MQ=255)                              ggaTGGCTCAGGGGCAATTTTTACAAGTTCCGTTCCTGACCGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACg                                                                                                                   <  2:1364019/147‑1 (MQ=255)                              gaAGGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACg                                                                                                                   <  2:1512423/149‑1 (MQ=255)                               aaTGGCTAAGGGGCAATCTTTACCAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACgg                                                                                                                  <  1:1056643/149‑1 (MQ=255)                               aaTGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACgg                                                                                                                  >  2:1058954/1‑149 (MQ=255)                                  ggCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCa                                                                                                               <  2:1061313/149‑1 (MQ=255)                                   gCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGGCAg                                                                                                              >  1:27542/1‑149 (MQ=255)                                     tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGcc                                                                                                            >  1:817286/1‑149 (MQ=255)                                     tAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGCTCCAGTTTCTATTTCTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAACACACGGTCAGcc                                                                                                            >  2:941921/1‑149 (MQ=255)                                           gCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGGGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATgg                                                                                                      >  1:1040626/1‑149 (MQ=255)                                             aattcttACAAGATCCGTTCCTTAACGCACGGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                                                                                    <  1:642181/144‑1 (MQ=255)                                             aaTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGtt                                                                                                    <  1:232497/149‑1 (MQ=255)                                              aTCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGttt                                                                                                   <  2:1586130/149‑1 (MQ=255)                                                ttttACAAGGTCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTAc                                                                                                 <  2:518836/148‑1 (MQ=255)                                                cTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTAc                                                                                                 <  1:938446/149‑1 (MQ=255)                                                      aaGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAc                                                                                           >  2:809090/1‑149 (MQ=255)                                                        gATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCAcgc                                                                                         <  1:146442/149‑1 (MQ=255)                                                              ttCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTc                                                                                   >  1:1453893/1‑149 (MQ=255)                                                               tCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCt                                                                                  <  2:740218/149‑1 (MQ=255)                                                                 cTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGAAtactac                                                                                >  2:76062/1‑149 (MQ=255)                                                                   gAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACtg                                                                              >  1:255489/1‑149 (MQ=255)                                                                    aaCGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTCCtgt                                                                             >  2:1573193/1‑149 (MQ=255)                                                                      cGCACTGCGGCGGGGACGTGTGCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAACTCGAGTCTTGTGGTCAGTTCGTGATCCTGTTGAAAAATACGTTCATCCAGATGGTTTACAAGAGCCCGATTTCTCCtgttt                                                                           >  2:831262/1‑148 (MQ=255)                                                                         gcTGCGGCGGGAATGTGTTTCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTcc                                                                        <  2:1512443/148‑1 (MQ=255)                                                                           tGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCg                                                                      >  2:1118272/1‑149 (MQ=255)                                                                            gcgcCGGGAACGTGTTCCAGTTTCTATTTAGTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCCTGTGATAAGTTCGTGAACAAGTAGAAAAACACGGTCAGCCAGATAGTTTAAAAGCACGCGATTTCTACTGTTGTCCCGt                                                                     <  2:1082609/149‑1 (MQ=255)                                                                             cGTCGGGACCGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAACAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGtc                                                                    <  1:1037161/149‑1 (MQ=255)                                                                               tCGGGAACGTGTTCCAGTTTCTATCTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGCTTTCTACTGTTGTCCCGtctc                                                                  <  2:1420853/149‑1 (MQ=255)                                                                                        tgtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCCGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGttt                                                         >  2:1414378/1‑149 (MQ=255)                                                                                         gtTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTCGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtc                                                        >  2:549034/1‑149 (MQ=255)                                                                                          ttCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtct                                                       >  1:1481216/1‑149 (MQ=255)                                                                                             cAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcat                                                    <  1:809090/149‑1 (MQ=255)                                                                                               gtgtCTATTTATTTTGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatca                                                  <  2:1436830/146‑1 (MQ=255)                                                                                               gtgtCTATTTATTGGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatca                                                  <  2:745658/146‑1 (MQ=255)                                                                                               gTTTCTATTTGTTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCtcatca                                                  <  1:41915/149‑1 (MQ=255)                                                                                                     atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtct                                                       <  2:1515902/138‑1 (MQ=255)                                                                                                     atttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTtct                                                       >  1:1515902/1‑138 (MQ=255)                                                                                                      tttatttGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCACGTCTCGCCCGGTTTCTCATCACAGTaac                                           >  1:365535/1‑149 (MQ=255)                                                                                                             ggTTAATGGTATTAAGCTGCAAGGGCAAATCGAGGCTTGTGATCAGGTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCg                                    <  2:1299650/149‑1 (MQ=255)                                                                                                                   tGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCg                              <  1:672687/149‑1 (MQ=255)                                                                                                                   tGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCg                              <  1:1058954/149‑1 (MQ=255)                                                                                                                   tGGTATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAAAGCCGGTGGCg                              <  1:842029/149‑1 (MQ=255)                                                                                                                      tATTAAGCTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACAAGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTGCCGTCTCGCCCGGTTTCTAATCACAGTAACAACGCCGACGGCgatc                           >  2:400863/1‑146 (MQ=255)                                                                                                                             cTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAg                                               >  1:566161/1‑122 (MQ=255)                                                                                                                             cTGCAAGGGCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAg                                               <  2:566161/122‑1 (MQ=255)                                                                                                                                    ggCAAATCGTGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCCCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTAc             <  1:755908/149‑1 (MQ=255)                                                                                                                                    ggCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCGCAGTAACAACGACGGTGGCGGTACCAGCAGTAACTAc             <  2:1481216/149‑1 (MQ=255)                                                                                                                                    ggCAAATCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGGGGCGGTACCAGCAGTAACTAc             >  1:1315107/1‑149 (MQ=255)                                                                                                                                       aaaTCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGGGGTACCAGCAGTAACTACtat          >  1:544618/1‑149 (MQ=255)                                                                                                                                          tCGAGTCTTGTGATCATTCCGTAATCCTGTTGAAAAACACGGTCAGCCAGATTGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcatcat       <  2:456038/149‑1 (MQ=255)                                                                                                                                          tCGAGTCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACcatcat       >  1:1104104/1‑149 (MQ=255)                                                                                                                                           cGAGTCTTGGGATCCGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATg      <  2:941965/149‑1 (MQ=255)                                                                                                                                               tCTTGTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTag  >  1:280114/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                 AGTACAAATAAGCATATAAGGAAAAGAGAGAATGGCTAAGGGGCAATCTTTACAAGATCCGTTCCTGAACGCACTGCGTCGGGAACGTGTTCCAGTTTCTATTTATTTGGTGAATGGTATTAAGCTGCAAGGGCAAATCGAGTCTTTTGATCAGTTCGTGATCCTGTTGAAAAACACGGTCAGCCAGATGGTTTACAAGCACGCGATTTCTACTGTTGTCCCGTCTCGCCCGGTTTCTCATCACAGTAACAACGCCGGTGGCGGTACCAGCAGTAACTACCATCATGGTAG  >  NC_000913_3_pae_pgi/4399517‑4399807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑