Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 1,731,621 G→T M845I (ATG→ATT lhr → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi1,731,6210GT98.8% 275.8 / NA 81M845I (ATG→ATTlhrputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (49/30);  total (50/31)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGT  >  NC_000913_3_pae_pgi/1731475‑1731764
                                                                                                                                                   |                                                                                                                                               
cATCGCACCCCGCTTTGGCAACCCCGGCGGCGCTGCGGCAGAATGCTGAAAGTCACTCAGGGATATCCAGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCg                                                                                                                                               <  1:368328/149‑1 (MQ=255)
cATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCCCTGGAACGTTTGATTCg                                                                                                                                               >  1:1076824/1‑149 (MQ=255)
  tCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                             >  1:67770/1‑149 (MQ=255)
  gcccACCCAGCTTCGGCAACAACGGCTCCGCGCCAGTCAGGTGCCGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGCAACCCTACTCGAATGTCTGCAAGAGGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTc                                                                                                                                             <  2:500128/146‑1 (MQ=255)
    gCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                           <  1:1061193/149‑1 (MQ=255)
        cccGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTGTCGAAACCCTACGCGAATGTTGTTGTTAAGGGGAAGATCTTCCGGCACTTGGACGTTTGATTCGTCGCCTGa                                                                                                                                       >  1:1570573/1‑149 (MQ=255)
          cGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAAc                                                                                                                                     >  1:424161/1‑149 (MQ=255)
             tttGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGt                                                                                                                                  <  2:248604/149‑1 (MQ=255)
                  caacaaCGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATCCGTCGCCTGAACGGTGGCGa                                                                                                                             >  1:247972/1‑149 (MQ=255)
                   aacaacGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGaa                                                                                                                            >  2:510586/1‑149 (MQ=255)
                    acaacGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGaaa                                                                                                                           <  1:1433421/149‑1 (MQ=255)
                      aacGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAAtt                                                                                                                         >  2:482377/1‑149 (MQ=255)
                       gcGGCCGAGCTCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGAATTTCCGGTCATTCTAGACACCCTACGCGAATGTCTGCCAGATGTTTATGACCTGCCCGCACTGGAACGTTTGATTCGTCGCCTAAGCGCTGGCGAAATTc                                                                                                                        <  2:1456662/148‑1 (MQ=255)
                         ggCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaa                                                                                                                      <  1:198099/149‑1 (MQ=255)
                          gCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATCTCCGGAGTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                     <  1:1504839/149‑1 (MQ=255)
                          gCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                     >  2:1097219/1‑149 (MQ=255)
                          gCTGCGCGCCAGGCAGTGGCTGGAAAGCGCTCAGGGATATCCGGATTTTCCGGTCATTCCCGAAACCCTACGCGAATGTCTGCAAGCTGTTTATTATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCaaa                                                                                                                     <  1:146578/149‑1 (MQ=255)
                           cTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTACCGGCCGCGCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAat                                                                                                                    >  1:721643/1‑149 (MQ=255)
                            tGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAata                                                                                                                   >  2:1205412/1‑149 (MQ=255)
                               gcggCAGTCAGGTGCTGGAAATCGCTTAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATcc                                                                                                                <  2:701280/149‑1 (MQ=255)
                                 gcCAGTCAGTGGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGCTCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGa                                                                                                              <  1:863866/149‑1 (MQ=255)
                                  ccAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGAt                                                                                                             <  1:263160/149‑1 (MQ=255)
                                    agtcagtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGt                                                                                                           <  1:1000413/149‑1 (MQ=255)
                                         gtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGa                                                                                                      >  1:266485/1‑149 (MQ=255)
                                         gtTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGa                                                                                                      >  2:294496/1‑149 (MQ=255)
                                              cTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCAc                                                                                                  >  2:1264224/1‑149 (MQ=255)
                                                 gAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATTTACGGACCACTAc                                                                                               >  2:785721/1‑149 (MQ=255)
                                                 gAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGATTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGGACGGTGGCGAAATTCAAATATACGGGGTCACGCCCACTAc                                                                                               >  2:35544/1‑149 (MQ=255)
                                                      cGCTCAGGGTTAGCCTGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCt                                                                                          <  1:557038/149‑1 (MQ=255)
                                                       gCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTc                                                                                         <  1:609031/149‑1 (MQ=255)
                                                           aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAACTATCCGATGTAACGACCACTACGCCCCCGcgt                                                                                     >  1:1514584/1‑147 (MQ=255)
                                                           aGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTAATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCt                                                                                     >  2:180950/1‑149 (MQ=255)
                                                                   ccGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCATTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGCAATTCAAAGTTCCGATGTAACGACCACTACGCCCTCGCCTTTCGCcac                                                                             <  2:1415231/149‑1 (MQ=255)
                                                                   ccGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCACGCCTTTcccccc                                                                             >  2:1601818/1‑147 (MQ=255)
                                                                    cGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTCACGACCACTGCGCCCTCGCCTTTCGCcaca                                                                            >  2:1542876/1‑149 (MQ=255)
                                                                      gATTTTCCGGTCATTCTCGAAACCCGACGCGTATGTCTACACGATGTTTATGATCTTCCCGCACTGCAACGTTTGATTCGTCGCCTGGACTGTGGCGAAATTCAAATATCCGATGTACCGACCACTACGCCCTCGCCTTTCGCCACCAg                                                                          >  2:421336/1‑149 (MQ=255)
                                                                          ttCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAACTTCAAATATTCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTa                                                                      <  2:1076824/149‑1 (MQ=255)
                                                                              ggTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTCCGCCCTCGCCTTTCGCCACAAGTTTATTGt                                                                  >  2:502578/1‑149 (MQ=255)
                                                                                  aTTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCgg                                                              >  2:131385/1‑149 (MQ=255)
                                                                                    tctcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCt                                                            >  1:1498335/1‑149 (MQ=255)
                                                                                      tcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCttt                                                                                   >  2:473660/1‑124 (MQ=255)
                                                                                      tcGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCttt                                                                                   <  1:473660/124‑1 (MQ=255)
                                                                                            cccTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACACCCTCCCCTTTCGCCACAAGTTTATTGTTCGGCTCTGTcggg                                                    >  1:851614/1‑147 (MQ=255)
                                                                                              cTACGTGAATGTTTGCAAGATGTTTATGATTTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGa                                                  <  2:1570573/149‑1 (MQ=255)
                                                                                               tACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGaa                                                 <  2:67770/149‑1 (MQ=255)
                                                                                                   cgAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTa                                             <  1:482377/149‑1 (MQ=255)
                                                                                                    gAATGTCTGCAAGATGTTTATGATCTCCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTAt                                            <  1:785721/149‑1 (MQ=255)
                                                                                                          cTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCa                                      <  1:1205412/149‑1 (MQ=255)
                                                                                                           tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc                                                             <  1:732061/125‑1 (MQ=255)
                                                                                                           tGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGc                                                             >  2:732061/1‑125 (MQ=255)
                                                                                                            gCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCaga                                    >  1:588989/1‑149 (MQ=255)
                                                                                                             cAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTTTTCGGCTATGTCGCGGAATTTATGTACGagag                                   >  1:1050528/1‑149 (MQ=255)
                                                                                                             cAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCagag                                   <  2:1498335/149‑1 (MQ=255)
                                                                                                             cAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCagag                                   >  1:1558368/1‑149 (MQ=255)
                                                                                                              aaGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGc                                  >  1:1150328/1‑149 (MQ=255)
                                                                                                               aGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                           <  2:1330636/43‑1 (MQ=255)
                                                                                                               aGATGTTTATGATCTTCCCGCACTGGAACGTTTGATTCGTCGc                                                                                                                                           >  1:1330636/1‑43 (MQ=255)
                                                                                                                      tATGATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGcccc                          >  2:91048/1‑149 (MQ=255)
                                                                                                                         gATCTTCCGGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATGGTTCGGCTATGTCGCGGAATTTATTTACCAGAGCGGCGCCCCGCt                       >  2:217280/1‑149 (MQ=255)
                                                                                                                         gATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGtt                                                                        <  2:283685/100‑1 (MQ=255)
                                                                                                                         gATCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGtt                                                                        >  1:283685/1‑100 (MQ=255)
                                                                                                                         gATCTTCCCGCACTGGAACGTTTGAATCGTCGCCTGAACGGTGGCGTAATTCAAATATCCGATGTAATGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAACTTATGTACCAGAGCGACGTCCCGCt                       >  1:1456319/1‑149 (MQ=255)
                                                                                                                           tCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTgg                     <  1:860793/149‑1 (MQ=255)
                                                                                                                           tCTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTgg                     >  1:973290/1‑149 (MQ=255)
                                                                                                                            cTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAc                                                                                                     >  2:1521224/1‑68 (MQ=255)
                                                                                                                            cTTCCCGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGAc                                                                                                     <  1:1521224/68‑1 (MQ=255)
                                                                                                                               cccGGACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCaga                 <  2:1186480/149‑1 (MQ=255)
                                                                                                                                ccGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGCTGTAACGACCACTACGCCCTCGCCTTTCGCCAAAAGTTTATTGTTCGGCTATGTCGCCGAATTTATGTACCAGAGCGACGACCCGCCGGCcgac                >  1:703799/1‑148 (MQ=255)
                                                                                                                                 cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACTAGAGCGACGCCCCGCTGGCAGAgc               >  1:1100280/1‑149 (MQ=255)
                                                                                                                                 cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGGgc               >  1:473859/1‑149 (MQ=255)
                                                                                                                                 cGCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAgc               >  1:1067632/1‑149 (MQ=255)
                                                                                                                                  gCACTGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTGTTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAgcg              >  1:1339223/1‑149 (MQ=255)
                                                                                                                                  gCACTGGAACGTTTGATTCGTCGCCTGAACGGTGACGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGACCGACGCCCCGCTGGCAGAgcg              >  2:1275394/1‑149 (MQ=255)
                                                                                                                                      tGGAACGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTACCGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCgg          >  2:1580583/1‑149 (MQ=255)
                                                                                                                                      tGGAACGTTTGATGCCTCGCCTGAACGGGGGCGCAATGCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCgg          <  1:297325/149‑1 (MQ=255)
                                                                                                                                        gAACGTTTGATTCGTCGCCTGAACGGTGGCGAAACTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCTGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGc        >  2:1278122/1‑149 (MQ=255)
                                                                                                                                         aaCGTTTGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCa       >  1:1264110/1‑149 (MQ=255)
                                                                                                                                          aCGTTTTATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCAt      >  1:205337/1‑149 (MQ=255)
                                                                                                                                             tttGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCg   >  2:473743/1‑149 (MQ=255)
                                                                                                                                             tttGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCg   >  1:1242210/1‑149 (MQ=255)
                                                                                                                                              ttGATTCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTGGCCTTTCGCCACAAGTTTATTGTTCGCCGATTTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGt  >  1:132787/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
CATCGCACCCCGCTTTGGCAACAACGGCTGCGCGCCAGTCAGTTGCTGGAAATCGCTCAGGGATATCCGGATTTTCCGGTCATTCTCGAAACCCTACGCGAATGTCTGCAAGATGTTTATGATCTTCCCGCACTGGAACGTTTGATGCGTCGCCTGAACGGTGGCGAAATTCAAATATCCGATGTAACGACCACTACGCCCTCGCCTTTCGCCACAAGTTTATTGTTCGGCTATGTCGCGGAATTTATGTACCAGAGCGACGCCCCGCTGGCAGAGCGCCGGGCATCCGT  >  NC_000913_3_pae_pgi/1731475‑1731764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: