Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913_3_pae_pgi 4,234,667 Δ1 bp intergenic (‑1434/‑499) lysC ← / → yjbE lysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913_3_pae_pgi4,234,6660A.98.5% 282.6 / ‑0.3 68intergenic (‑1433/‑500)lysC/yjbElysine‑sensitive aspartokinase 3/extracellular polysaccharide production threonine‑rich protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base . (28/36);  total (31/37)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GTCTATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_pae_pgi/4234519‑4234809
                                                                                                                                                   |                                                                                                                                               
gTCTATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGat                                                                                                                                                <  1:697367/149‑2 (MQ=255)
 tCTATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatc                                                                                                                                               >  2:18464/1‑147 (MQ=255)
  cTATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatca                                                                                                                                              >  1:112197/1‑146 (MQ=255)
   tatataCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatcat                                                                                                                                             <  1:18464/149‑5 (MQ=255)
   tatataCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGatcat                                                                                                                                             >  1:622932/1‑145 (MQ=255)
     tataCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcg                                                                                                                                          >  1:303231/1‑149 (MQ=255)
      ataCGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgt                                                                                                                                         <  1:763562/149‑1 (MQ=255)
      ataCGCAGTAGAGATGCACCCCAAGGGCGTTTTAGCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCAtcgt                                                                                                                                         <  1:395810/149‑1 (MQ=255)
         cGCAGGAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGa                                                                                                                                      <  2:877496/149‑1 (MQ=255)
          gCAGTAGAGATGCACCCCAAGGGCGTTTTCTACTTTACACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGat                                                                                                                                     >  1:784660/1‑149 (MQ=255)
           cAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGTTTAGAGGCGTAAATCCCCTGGCCACCCCGCGAATAGATGCCTTGAAGAGGCGATTGCACTGATCATGCTCGata                                                                                                                                    >  2:619649/1‑149 (MQ=255)
            aGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCACCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGCCGatcc                                                                                                                                   >  2:987881/1‑147 (MQ=255)
                  gaTGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGCCGATATGTAGGc                                                                                                                             >  1:69054/1‑149 (MQ=255)
                      cACCCCAATGCCCTTTTATTCTTTTTACGAGATGTGGGCATCCCCTCGTTAAAACTGGAAGAGATTAGAGGCCGAAACCCCCTGGCCTCCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCggc                                                                                                                         <  2:918478/149‑2 (MQ=255)
                         cccAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATaa                                                                                                                      >  2:24177/1‑149 (MQ=255)
                          ccAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAg                                                                                                                     <  2:480872/149‑1 (MQ=255)
                            aaGGGCCTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                   <  1:29581/149‑1 (MQ=255)
                               ggCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGcc                                                                                                                            <  2:766997/137‑1 (MQ=255)
                               ggCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGcc                                                                                                                            >  1:766997/1‑137 (MQ=255)
                                 cGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATACAGGCATGGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAAGACGGGAAAGGCGtgta                                                                                                              >  2:254771/1‑146 (MQ=255)
                                    tttATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                           >  2:956364/1‑149 (MQ=255)
                                    ttcaTCCTTTTTTCGAGATTTGGGCATCGCCTTTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCAcgc                                                                                                           <  1:340320/146‑1 (MQ=255)
                                     ttATCCTTTTTATGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCCATGAACATCGTCGATCTTTTGGACGGATAATGCGTTAAcacc                                                                                                          >  2:100864/1‑149 (MQ=255)
                                             tttACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                  <  1:283978/149‑1 (MQ=255)
                                               tACGAGATGTGGGCATCGCCTCTGTAAAACGGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGTATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                <  2:27372/149‑2 (MQ=255)
                                                aCGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCaa                                                                                               <  2:784660/149‑1 (MQ=255)
                                                    caTGGGGGCATCGCCTCGGTCAAACTGGCAAGGATTACAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATACCCATCCAGGGTTAGGCGTTCACGCCGTATCCGGCAACCGa                                                                                           <  2:230106/148‑1 (MQ=255)
                                                     aTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                          <  1:956364/149‑1 (MQ=255)
                                                     aTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCAGCAACCGAt                                                                                          >  1:46354/1‑149 (MQ=255)
                                                     aTGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAAAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGAt                                                                                          >  2:742368/1‑149 (MQ=255)
                                                        tgGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGCTCATCGTCGATATGTAGGCCGGGTAAGGCGTTCACGCCGCATCCGGCAACCGATGcc                                                                                       >  1:748760/1‑149 (MQ=255)
                                                           gCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                                    >  1:234424/1‑149 (MQ=255)
                                                           gCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGa                                                                                    <  1:24177/149‑1 (MQ=255)
                                                                 ccTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                              <  1:868699/149‑1 (MQ=255)
                                                                 ccTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGAc                                                                              >  1:957628/1‑149 (MQ=255)
                                                                  ctctGTAAAACTGGCAGAGATTAGACGCGTGCATTCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGCCGATATGTAGGCCGGATACGCCGTTCACGCCGCATCCGGCAACCCATGCATGATGCGAca                                                                             >  2:758746/1‑148 (MQ=255)
                                                                            cgggCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTc                                                                   <  2:687020/147‑1 (MQ=255)
                                                                             tGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCt                                                                  <  1:854586/149‑1 (MQ=255)
                                                                             tGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGATTGCAGGGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCt                                                                  >  1:988236/1‑149 (MQ=255)
                                                                               gCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                   >  1:379211/1‑98 (MQ=255)
                                                                               gCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGc                                                                                                                   <  2:379211/98‑1 (MQ=255)
                                                                                  gagaTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                             <  1:806060/149‑1 (MQ=255)
                                                                                  gagaTTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCa                                                             <  1:254771/149‑1 (MQ=255)
                                                                                      ttAGGGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGGATTGAGGAGGCGCGTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                         <  1:673160/149‑1 (MQ=37)
                                                                                      ttAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGACAGATAATTCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGcc                                                         <  1:987881/149‑1 (MQ=38)
                                                                                          agGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACa                                                     <  1:610348/149‑1 (MQ=255)
                                                                                            gCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAgg                                                   >  1:51993/1‑149 (MQ=255)
                                                                                             cGTAAATCCCCGGGGCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGt                                                  <  2:148355/149‑1 (MQ=255)
                                                                                               tAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACACGTCg                                                >  2:696364/1‑149 (MQ=255)
                                                                                                aaaCCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGa                                               <  1:723618/149‑1 (MQ=255)
                                                                                                   tCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGc                                            <  1:707813/149‑1 (MQ=255)
                                                                                                      ccTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGTTTGCAGTGATCATCGTGGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACATGTCGATGCCGa                                         <  2:256825/149‑1 (MQ=255)
                                                                                                       cttgCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCATTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGGCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGat                                        <  2:748760/146‑1 (MQ=255)
                                                                                                           ccACACCGAGAATAGATGTGTTGTTGAGGCGCATGCAGAGATCATCTTCGATAGGTAGGTCGGATGAGGCGTTCAGGCCGCATCGGGCAAGCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGTTGCGGATATGt                                    >  2:887196/1‑149 (MQ=18)
                                                                                                             aCCCCGGGAATAGATGCATTGAAGAGGCGTTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCTTCTTATCAGGCCTACAGGTCGATGCCGATATGTAc                                  <  2:873468/149‑1 (MQ=255)
                                                                                                                 cGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCg                              >  2:544440/1‑149 (MQ=255)
                                                                                                                   agaATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTa                            >  2:390601/1‑149 (MQ=255)
                                                                                                                    gaATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                           <  2:932132/133‑1 (MQ=255)
                                                                                                                    gaATAGATGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGcc                                           >  1:932132/1‑133 (MQ=255)
                                                                                                                      aTAGATGCATTGAAGAGGCGCTTGCAGTCTTGCGCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTc                         >  1:525611/1‑149 (MQ=25)
                                                                                                                          aTGCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATACCGGTATGTACGTCGGATTCggaa                     >  2:489598/1‑147 (MQ=25)
                                                                                                                            gCATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCATGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAt                   <  2:917009/149‑1 (MQ=255)
                                                                                                                             cATTGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                  <  1:843096/149‑1 (MQ=255)
                                                                                                                             cATGGAAGAGGGGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAAtt                  <  2:303231/149‑1 (MQ=255)
                                                                                                                              gatctaaagGCGTTTGCAGTTATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCTTCCGGCAACCGATGCCGGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTa                 <  2:935514/142‑1 (MQ=25)
                                                                                                                               ttGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTaa                <  1:645664/149‑1 (MQ=255)
                                                                                                                                tGAAGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAAt               <  1:544440/149‑1 (MQ=255)
                                                                                                                                  aaGAGGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATAc             >  2:273864/1‑149 (MQ=255)
                                                                                                                                   agagGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACa            <  1:390601/149‑1 (MQ=255)
                                                                                                                                    gagGCGCTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGGTGCCTGATGCGACGCGGTCGCGTCCTATCAGGCCTACAGGTCGATACCGATATGTACATCGTATCTGGCAATTaaagatc           >  1:935514/1‑144 (MQ=25)
                                                                                                                                       gcgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATACCGATATGTACATCGTATTCGGCAATTAATACATAGc        >  2:342826/1‑149 (MQ=255)
                                                                                                                                        cgcTTGCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCa       >  1:183589/1‑149 (MQ=255)
                                                                                                                                             gCAGTGATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACgatt  >  1:567493/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
GTCTATATACGCAGTAGAGATGCACCCCAAGGGCGTTTTATCCTTTTTACGAGATGTGGGCATCGCCTCTGTAAAACTGGCAGAGATTAGAGGCGTAAATCCCCTGGCCACCCCGAGAATAGATGCATTGAAGAGGCGCTTGCAGTGAATCATCGTCGATATGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAACCGATGCCTGATGCGACGCGGTCGCGTCTTATCAGGCCTACAGGTCGATGCCGATATGTACATCGTATTCGGCAATTAATACATAGCACGATT  >  NC_000913_3_pae_pgi/4234519‑4234809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: