Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913_3_pae_pgi	1,978,503	Δ776 bp		insB1–insA	insB1, insA

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913_3_pae_pgi	1978503–1979270	1979278	9–776	38 [0]	[0] 37	insB1–insA	insB1,insA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913_3_pae_pgi	= 1978502	0 (0.000)	37 (0.660)	34/262	0.9	100%	intergenic (‑305/+16)	flhD/insB1	flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
	?	NC_000913_3_pae_pgi	1979279 =	0 (0.000)					intergenic (‑64/‑474)	insA/uspC	IS1 repressor TnpA/universal stress protein

TAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCC  >  NC_000913_3_pae_pgi/1978354‑1978642                                                                                                                                                     |                                                                                                                                             tAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATG                                                                                                                                              >  2:169557‑M1/1‑149 (MQ=255)    aaaTGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtat                                                                                                                                           >  1:1004110‑M1/1‑146 (MQ=255)              ttACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatact                                                                                                                                 >  1:909909‑M1/1‑136 (MQ=255)               tACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactt                                                                                                                                >  2:416830‑M1/1‑135 (MQ=255)                    ttATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtt                                                                                                                           <  1:416830‑M1/149‑20 (MQ=255)                       tCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATCACGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATATATGtataagtcatacttttgttttg                                                                                                                        <  2:864231‑M1/149‑23 (MQ=255)                        cTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgg                                                                                                                       <  2:1004110‑M1/149‑24 (MQ=255)                        cTAAGATTTTTCACAAATCGACGCAACTGTACTCGTCACTACACGCACTTACAACGGAGGGGGCCTTCGATTTTCAATAATGCGTGCTGCAGATCACACCAAACACTCAATTACTTAACTTAAATGtataaggcttatttttgttttgg                                                                                                                       <  2:952324‑M1/149‑24 (MQ=255)                                tttCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatactttttttttgggtgtattt                                                                                                               >  1:170309‑M1/1‑118 (MQ=255)                                        aTCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgt                                                                                                       >  2:412174‑M1/1‑110 (MQ=255)                                            aCGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaa                                                                                                   >  2:594628‑M1/1‑106 (MQ=255)                                                aaCTGTACTCGTCACTACACGCACAAACAACGGCGGGGAGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTAATTAACATAAATGtataagtaatacttttgttttgggtgtatttgaatttgttaagaggt                                                                                               >  2:470077‑M1/1‑102 (MQ=255)                                                      aCTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgtgttgggtgtatttcaatctgttaaaaagtttttcg                                                                                         >  1:913444‑M1/1‑96 (MQ=255)                                                          gTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttccctac                                                                                     >  2:522730‑M1/1‑92 (MQ=255)                                                              cTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgcga                                                                                 >  2:906274‑M1/1‑88 (MQ=255)                                                                  acGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtagttcaatctgttaaaaagtttttcgctacgctagcaa                                                                             >  1:725423‑M1/1‑84 (MQ=255)                                                                    gCACATACAACGGAGGGGGGCTGCGATTTGCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagc                                                                           >  2:276365‑M1/1‑82 (MQ=255)                                                                            aaCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatga                                                                   >  2:1030566‑M1/1‑74 (MQ=255)                                                                             aCGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttgaaaagttttacgctacgctagcaagcaaaaatgaa                                                                  >  1:400715‑M1/1‑73 (MQ=255)                                                                                gAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaaca                                                               <  1:276365‑M1/149‑80 (MQ=255)                                                                                 aGGGGTGCTGCAATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacag                                                              <  2:889681‑M1/149‑81 (MQ=255)                                                                                  ggggggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacagg                                                             <  1:412174‑M1/149‑82 (MQ=255)                                                                                     gggCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACACAAATGtataagtcatacttttgttttgggtgtacttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaat                                                          <  1:571045‑M1/149‑85 (MQ=255)                                                                                     gggCTGCGATTTTCAATAAAGCGTGATGCAGATCACACAAAACACTCAATCACTTAACATAAATGtataagtcatacttttgttttgggtgtctttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaat                                                          <  1:570418‑M1/149‑85 (MQ=255)                                                                                                  cAATAAGGTGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatggga                                             <  2:623899‑M1/149‑98 (MQ=255)                                                                                                   aataatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggat                                            >  2:455918‑M1/1‑51 (MQ=255)                                                                                                      aatGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgtt                                         <  1:169557‑M1/149‑102 (MQ=255)                                                                                                        tgccttatgcagaTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttctctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgc                                       <  1:477774‑M1/143‑104 (MQ=255)                                                                                                        ggcagtatgcagaTAACAGAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgc                                       <  2:484270‑M1/143‑104 (MQ=255)                                                                                                               tGCAGATCCCCCAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagt                                >  2:658192‑M1/1‑39 (MQ=255)                                                                                                                    aTCACACAAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttcggtgtatttcaatctgttaaaaagtgtttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcaccgtcaaaa                           >  2:910263‑M1/1‑34 (MQ=255)                                                                                                                       acacaAAACACTCAATTACTTAACATAAATGtctaagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaacatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataa                        >  1:601983‑M1/1‑31 (MQ=255)                                                                                                                        cacaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataac                       <  1:658192‑M1/149‑120 (MQ=255)                                                                                                                         acaAAACACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataact                      <  1:522730‑M1/149‑121 (MQ=255)                                                                                                                           aaaaCACTCAATTACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgtgcacagtcaaaataactca                    >  1:796955‑M1/1‑27 (MQ=255)                                                                                                                                       tACTTAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatc        <  1:564709‑M1/149‑135 (MQ=255)                                                                                                                                           tAACATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaatgatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatct    <  2:913444‑M1/149‑139 (MQ=255)                                                                                                                                             aCATAAATGtataagtcatacttttgttttgggtgtatttcaatctgttaaaaagtttttcgctacgctagcaagcaaaaatgaaacaggaataatcgaaatgggatgttgcgcacagtcaaaataactcaccgtaaataatcatctgc  >  2:916865‑M1/1‑9 (MQ=255)                                                                                                                                                     |                                                                                                                                             TAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCC  >  NC_000913_3_pae_pgi/1978354‑1978642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑