New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi = 353067036 (1.140)4 (0.140) 4/268 4.0 10.9% intergenic (+18/+45) hslO/yhgE heat shock protein Hsp33/DUF4153 family putative inner membrane protein
?NC_000913_3_vch_pgi = 3530674 32 (1.090)intergenic (+22/+41) hslO/yhgE heat shock protein Hsp33/DUF4153 family putative inner membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGT  >  NC_000913_3_vch_pgi/3530532‑3530810
                                                                                                                                          |                                                                                                                                            
ggCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGc                                                                                                                                    <  2:263350/149‑1 (MQ=255)
  cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                  <  1:505035/149‑1 (MQ=255)
  cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                  <  1:504933/149‑1 (MQ=255)
  cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                  <  1:301126/149‑1 (MQ=255)
            cGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                        <  2:314560/149‑1 (MQ=255)
            cGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGa                                                                                                                        <  1:397136/149‑1 (MQ=255)
                     cATGCAGTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATacac                                                                                                               <  1:130289/149‑1 (MQ=255)
                       tGCATTGTGATTACTGCGGTAACCACTATCTGTCCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCt                                                                                                             <  2:243403/149‑1 (MQ=255)
                           ttGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGGCTAATACACCTGATg                                                                                                         >  1:313863/1‑149 (MQ=255)
                           ttGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATAGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATg                                                                                                         <  2:453441/149‑1 (MQ=255)
                             gtgATTACTGCGGTAACCACTATCTGATCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCGGGCAGATCCGCAAGTGCATTAATGAGTATGTCCGGCAGAGAGGACTCTGCCGGATTTTCCGACTACTACACCTGATgcg                                                                                                       >  2:41991/1‑149 (MQ=255)
                                    cTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                <  1:293821/149‑1 (MQ=255)
                                      gCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCTCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATacac                                                                                                               <  1:468260/132‑1 (MQ=255)
                                      gCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATacac                                                                                                               >  2:468260/1‑132 (MQ=255)
                                          tAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGGTTGGACACTCAt                                                                                          >  1:532781/1‑149 (MQ=255)
                                            aCCACTATCTTTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTGACGACTAATACACCTGATGCGCTTTGTCACTCATTc                                                                                        <  1:271802/149‑1 (MQ=255)
                                                   tCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCTAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTca                                                                                 <  2:511413/149‑1 (MQ=255)
                                                   tCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTca                                                                                 >  1:77444/1‑149 (MQ=255)
                                                      gTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACAt                                                                              >  1:529698/1‑149 (MQ=255)
                                                          aaTGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCgg                                                                                                                                 >  2:280855/1‑94 (MQ=255)
                                                             gCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCt                                                                       <  2:145808/149‑1 (MQ=255)
                                                               gagggATATGGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCttt                                                                     <  1:2512/146‑1 (MQ=255)
                                                                aTGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTc                                                                    >  1:445584/1‑149 (MQ=255)
                                                                  ggATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTccc                                                                  >  2:296568/1‑147 (MQ=255)
                                                                   gATATTGCTGAACTCCGCAACAACGCGTCTCCGGCAGATCCGCACGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCAcc                                                                 <  1:103813/149‑1 (MQ=255)
                                                                   gATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCAcc                                                                 <  1:57991/149‑1 (MQ=255)
                                                                           tGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTa                                                         >  2:150606/1‑149 (MQ=255)
                                                                           tGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCAATCAGAGTTACATTCAGCCTTTAACCATCCACtc                                                         >  2:500242/1‑148 (MQ=255)
                                                                              aaTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACaa                                                      <  1:287139/149‑1 (MQ=255)
                                                                               aTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAg                                                     >  1:535211/1‑149 (MQ=255)
                                                                                                tcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAg                                    >  1:175162/1‑149 (MQ=255)
                                                                                                   ggCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCt                                 <  2:535211/149‑1 (MQ=255)
                                                                                                              aaGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGCTTTTACGACTAATACACCTGATGCGCTTTGTCAGTCATTCAGATTCACATTCAGCCTTTCACCATCAACCACAACATCGCGCCACGCTttagtcttagtctgtcgt                      >  2:113697/1‑143 (MQ=255)
                                                                                                               aGTTCAGTAATGAGTATCGCCGGCGGAGAGTGCTCTGTCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATCCACATTCAGCCTTTCACCCTCCACTACAGGATCGCGCCACGCTTTAGGCGTCGTCCCCAGtt                     <  2:147554/149‑1 (MQ=255)
                                                                                                                     ttAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCAt               >  1:201667/1‑149 (MQ=255)
                                                                                                                       aaTGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATcc             >  1:437507/1‑149 (MQ=255)
                                                                                                                                  ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgt  >  2:82554/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                            
GGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGT  >  NC_000913_3_vch_pgi/3530532‑3530810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.