Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913_3_vch_pgi 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913_3_vch_pgi 1299499–1300697 1300697 1–1199 31 [0] [0] 31 ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi = 12994980 (0.000)31 (1.010) 26/280 0.3 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913_3_vch_pgi 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

TGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATA  >  NC_000913_3_vch_pgi/1300552‑1300836
                                                                                                                                                  |                                                                                                                                          
cgcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttggccacatcataatcctgggcttgcgggagaataattgaaatgatattattaattccactgccgttggtagaggaaagtgctaaata                                                                                                                                          >  2:7372‑M2/147‑149 (MQ=255)
 tcaaattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataa                                                                                                                                         <  2:164892‑M2/4‑1 (MQ=255)
         cagttagttataaggatttccttaactgtttctcctcaccatcatgttattttcgccacatcataatcctggtcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                                 <  2:380965‑M2/12‑1 (MQ=255)
          agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                <  1:308829‑M2/13‑1 (MQ=255)
            ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                              >  1:390415‑M2/135‑149 (MQ=255)
                    aaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattat                                                                                                                      >  1:503411‑M2/127‑149 (MQ=255)
                     aggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTTTTAAattatt                                                                                                                     >  2:308852‑M2/126‑149 (MQ=255)
                           tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgacatgatattattaattccactgcctttgatagaggaaagtgctaaATAATAATCAATTGTTCAATTATTGTGCAt                                                                                                               >  2:310085‑M2/120‑149 (MQ=255)
                               taactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                           <  1:184571‑M2/34‑1 (MQ=255)
                                aactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggacagtgctaaATAATAATCAATGGTTAAATTATTGTGCATTTCac                                                                                                          <  2:480103‑M2/35‑1 (MQ=255)
                                   tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatcttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTATTCTTTTcaatat                                                                                                       >  1:456674‑M2/112‑148 (MQ=255)
                                       tctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaaatgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGa                                                                                                   <  1:488920‑M2/42‑1 (MQ=255)
                                                    atgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                      <  1:285441‑M2/55‑1 (MQ=255)
                                                         attttcgccatatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                 <  2:513927‑M2/60‑1 (MQ=255)
                                                             tcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAc                                                                             <  2:21488‑M2/64‑1 (MQ=255)
                                                                    atcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                     <  1:284906‑M2/24‑1 (MQ=255)
                                                                    atcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                     >  2:284906‑M2/79‑102 (MQ=255)
                                                                      cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                    >  1:386995‑M2/77‑149 (MQ=255)
                                                                          atcctgggattgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAGTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAt                                                                <  2:144466‑M2/77‑1 (MQ=255)
                                                                                 gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                         <  2:149738‑M2/84‑1 (MQ=255)
                                                                                          agaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                <  1:139656‑M2/93‑1 (MQ=255)
                                                                                              taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTATCCAATCTCTATTTGAttgaattgaa                                            <  1:136910‑M2/97‑1 (MQ=255)
                                                                                               acttgaaatgatattatgaattccactggagtttggagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                           <  2:107443‑M2/98‑1 (MQ=255)
                                                                                               aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                           <  1:236698‑M2/98‑1 (MQ=255)
                                                                                               aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                           <  1:195985‑M2/98‑1 (MQ=255)
                                                                                                                        ctgcctttgtgaaaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                  <  2:412921‑M2/123‑1 (MQ=255)
                                                                                                                        ctgcctttggtagaggaaagtgctaaATAATAATCAATTCTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGTGAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTGAAGGCATGGATGCaa                  <  2:454243‑M2/123‑1 (MQ=255)
                                                                                                                            ctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATAGAAAGCTGGTATTTAAGGCATGCATGCCagcga              >  1:541440‑M2/23‑147 (MQ=255)
                                                                                                                                      ggaaagttctaaATAATAATCAATTGTTAAATGATTGGGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGACAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa    <  2:461609‑M2/137‑1 (MQ=255)
                                                                                                                                        aaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa  <  1:485457‑M2/139‑1 (MQ=255)
                                                                                                                                        aaagtgctaaACAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAGCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa  <  1:208120‑M2/139‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                          
TGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATA  >  NC_000913_3_vch_pgi/1300552‑1300836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: