New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913_3_vch_pgi 3375666 =19 (1.040)4 (0.230) 4/272 1.9 18.6% coding (798/1368 nt) dcuD putative transporter
?NC_000913_3_vch_pgi 3375686 = 17 (0.980)coding (818/1368 nt) dcuD putative transporter
Rejected: Frequency below/above cutoff threshold.

AATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTC  >  NC_000913_3_vch_pgi/3375547‑3375831
                                                                                                                                           |                                                                                                                                                 
aatcaCGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTggtggtggt                                                                                                                                          <  2:111458/149‑1 (MQ=255)
           ggCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGa                                                                                                                               <  1:197447/149‑1 (MQ=255)
             cAGAGCAAAAAGCTCTCGATAATGTCCCGGCGCTCTATTACGCCATTTTACCTGCGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGt                                                                                                                             <  1:278390/149‑1 (MQ=255)
                  aaaaaacgcctgcacatcgtccattgtttcgcgcaagttatgcttgcggaagaactcaacaaacatcgtcacagtcaaactcagtaacatcaccaccaccaGATGCAGTCAGAACTGCATCtgg                                                                                                                                                 <  2:243026‑M1/23‑1 (MQ=255)
                  aaaaaacgcctgcacatcgtccattgtttcgcgcaagttatgcttgcggaagaactcaacaaacatcgtcacagtcaaactcagtaacatcaccaccaccaGATGCAGTCAGAACTGCATCtgg                                                                                                                                                 >  1:243026‑M1/102‑124 (MQ=255)
                                       ccgccgCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGtt                                                                                                   >  2:186159/1‑149 (MQ=255)
                                                     cGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCCGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCGTa                                                                                     >  1:303368/1‑149 (MQ=255)
                                                        cATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGAGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTGTGTTGAGTTCTTCCGCAAGCATAACt                                                                                  >  1:210261/1‑149 (MQ=255)
                                                            ttATCTGTGATGCCGTTAAGCCGGATGCTCGGCTCGCTGTTCCTCGTCCACGTCGGGCTTATGCAGATAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGCCGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTgcg                                                                              <  2:159430/149‑1 (MQ=255)
                                                                 tgtgATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTGTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGaaa                                                                         >  1:200949/1‑149 (MQ=255)
                                                                                ccTGATGCTAGTTTCGATGGACCTCGCCAACGTCGGGCCGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGc                                                          <  1:140703/149‑1 (MQ=255)
                                                                                 cTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGcg                                                         >  1:99042/1‑148 (MQ=255)
                                                                                 cTGATGCCCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCa                                                         <  1:255360/149‑1 (MQ=255)
                                                                                                actcagtaacatcaccaccaccaGATGCAGTCAGAACTGCATCtggtg                                                                                                                                               <  1:25605‑M1/25‑1 (MQ=255)
                                                                                                actcagtaacatcaccaccaccaGATGCAGTCAGAACTGCATCtggtg                                                                                                                                               >  2:25605‑M1/24‑48 (MQ=255)
                                                                                                   ttCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCAt                                       <  1:186159/149‑1 (MQ=255)
                                                                                                      cTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATggg                                    >  1:117323/1‑149 (MQ=255)
                                                                                                             aCGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAg                             <  1:304682/149‑1 (MQ=255)
                                                                                                                  caccagatgcagttctgactgcatcTGGTGGTGGTGATGTTACTGAGt                                                                                                                             <  1:25605‑M2/23‑1 (MQ=255)
                                                                                                                  caccagatgcagttctgactgcatcTGGTGGTGGTGATGTTACTGAGt                                                                                                                             >  2:25605‑M2/26‑48 (MQ=255)
                                                                                                                    gCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCATCCTACTATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCa                      <  1:144385/149‑1 (MQ=255)
                                                                                                                    ccagatgcagttctgactgcatcTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGtttttt                                               >  1:243026‑M2/24‑124 (MQ=255)
                                                                                                                    ccagatgcagttctgactgcatcTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGtttttt                                               <  2:243026‑M2/101‑1 (MQ=255)
                                                                                                                     cTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGCGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCCGTTTGccca                     >  2:185917/1‑147 (MQ=255)
                                                                                                                             GTCAGAACTGCATTTGGTGGTGGTGATGTTACTGAGTTTGGCTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTaa             >  2:72644‑M1/1‑149 (MQ=255)
                                                                                                                              TCAGAACTGAATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAAc            >  1:13159‑M1/1‑149 (MQ=255)
                                                                                                                                aGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGGCGTTTGCCAGCGTGGTAACGc          >  1:267293/1‑149 (MQ=255)
                                                                                                                                   actgcatcTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTCAGTTCTTCCGCAAGCATTACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCtgg       <  1:299042‑M2/141‑1 (MQ=255)
                                                                                                                                        atcTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTCAGCAGTTTGCCCACGTGTtaacgctgtgtgtc  >  2:48005‑M2/4‑143 (MQ=255)
                                                                                                                                           |                                                                                                                                                 
AATCACGAACAGGCAGAGCAAAAAGCTCTCGATAATGTCCCGCCGCTCTATTACGCCATTTTACCTGTGATGCCGTTAATCCTGATGCTCGGCTCGCTGTTCCTCGCCCACGTCGGGCTGATGCAGTCAGAACTGCATCTGGTGGTGGTGATGTTACTGAGTTTGACTGTGACGATGTTTGTTGAGTTCTTCCGCAAGCATAACTTGCGCGAAACAATGGACGATGTGCAGGCGTTTTTTGACGGCATGGGTACGCAGTTTGCCAACGTGGTAACGCTGGTGGTC  >  NC_000913_3_vch_pgi/3375547‑3375831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.